Isolation and Characterization of Malasezzia Species from Dandruff Samples and Determination of its Sensitivity Towards Antifungal Agents

Begum, Khadiza; Nur, Farjahan; Shahid, Mashmum Shababa

doi:10.3329/bpj.v22i2.42298

Cited by 9 publications

(8 citation statements)

References 12 publications

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“…Blast searches revealed Neurospora intermedia, Trichoderma atroviride, and Trichosporon asahii with a percent similarity of 100.00% each. The identification test results are in line with the work of Begum et al (2019) who used a similar approach to identify dandruff causing fungi. In a study conducted by Cernansky et al ( 2020), the authors revealed that Neurospora species are often found on humans as a result of exposure to contaminated food and plants, especially sugar cane.…”

Section: Fungi Iden Fica Onsupporting

confidence: 81%

Antidandruff property of Psidium guajava leaf extracts

Bhajan¹,

Govinden²,

Sanmukhiya³

2022

NRFHH

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Psidium guajava leaf paste and decoction have long been used in Mauritius for their efficacy in dandruff management. The study aimed to test P. guajava leaf extracts against fungi collected from dandruff patients' scalps, as previous research has shown a strong link between dandruff and dermatophytes. Fungi were isolated and cultured on Sabouraud dextrose agar (SDA), which contained 2% olive oil, 1% Tween 80, and chloramphenicol. The isolates were identified using physiological, macroscopic, microscopic, biochemical, and molecular techniques. Hydrodistillation and Soxhlet extraction of Mauritian P. guajava leaves were used to obtain essential oil and different solvent fractions, which were then screened for the presence of phytochemicals. The microdilution assay was used to test the antifungal activity of harvested extracts against isolates. Neurospora intermedia, Trichoderma atroviride, and Trichosporon asahii were identified as fungi. The solvents chloroform, methanol, and hexane produced higher extract yields (16.2%, 15.2% and 14.7%). The phytochemicals were most abundant in the chloroform-methanol (4:1 v/v) fraction. The methanol fraction had the highest total phenolic content (TPC), total flavonoid content (TFC), and total tannin content (TTC), with concentrations of 298.27 19.66 g GAE/g, 132.1 6.15 g QUE/g, and 118.53 1.60 g GAE/g, respectively. Isolates were most sensitive to chloroform-methanol (4:1 v/v) and methanol fractions, with a MIC of 1.56 mg/mL. From the study, it can be deduced that P. guajava leaf extracts are effective antifungal agents and potential ingredients for natural antidandruff hair products, which are in high demand in the cosmetic industry.

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Section: Fungi Iden Fica Onsupporting

confidence: 81%

Antidandruff property of Psidium guajava leaf extracts

Bhajan¹,

Govinden²,

Sanmukhiya³

2022

NRFHH

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“…M. pachydermatis dalam penelitian ini mendominasi di antara spesies jamur yang lain, yaitu mencapai 50,0%. M. pachydermatis juga pernah ditemukan pada dandruff yang diperoleh dari 25 sampel mahasiswa di Bangladesh (Begum K et al, 2019). M. pachydermatis adalah flora normal pada kulit hewan peliharaan dan hewan liar (Guillot J et al, 1997).…”

Section: Prosedur Penelitianunclassified

Dandruff Yang Disebabkan Jamur Dan Faktor Risiko Kejadian Pada Mahasiswa Teknologi Laboratorium Medis Poltekkes Kemenkes Jayapura

Wardani

Hartati

Purwati

et al. 2022

j. insan. cendekia.

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Dandruff adalah kondisi non-inflamasi yang ditandai dengan sisik putih hingga kekuningan yang terkelupas pada kulit kepala. Dandruff dapat disebabkan oleh faktor mikroba maupun non-mikroba. Malassezia adalah genus jamur flora normal pada kulit manusia dan sekaligus menjadi agen etiologi dandruff. Ada beberapa faktor risiko yang dapat mempengaruhi adanya dandruff, seperti perawatan rambut dan penggunaan hijab. Penelitian ini bertujuan untuk mengetahui kejadian dandruff yang disebabkan oleh jamur. mengetahui faktor risiko penggunaan hijab dan jenis kelamin terhadap kejadian dandruff. Isolasi jamur dilakukan dengan media sabouraud dextrose agar. Identifikasi jamur dilakukan melalui pengamatan secara makroskopis dan mikroskopis dengan pewarnaan lactophenol cotton blue. Faktor risiko penggunaan hijab dan jenis kelamin terhadap kejadian dandruff dianalisis dengan uji Chi square. Dari 49 responden, 9 responden tidak terdapat dandruff dan 40 responden terdapat dandruff. Dari 40 sampel dandruff, sebanyak 14 sampel dandruff ditemukan adanya jamur, yaitu Malassezia pachydermatis sebanyak 50,0%, M. restricta 27,8%, M. globosa 5,6%, M. sympodialis 5,6% dan Trichophyton mentagrophytes sebanyak 11,1%. Berdasarkan analisis statistik, terdapat hubungan antara penggunaan hijab terhadap kejadian dandruff. Kejadian dandruff lebih banyak terjadi pada responden tidak berhijab. Faktor perawatan rambut dan paparan sinar matahari, debu dan kotoran dapat mempengaruhi kejadian dandruff. Untuk faktor jenis kelamin tidak terdapat hubungan dengan kejadian dandruff.

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“…However, long‐term use of the same has certain negative consequences. Some of the chemicals routinely employed in formulations have been found to cause molecular harm to cells, resulting in genetic alterations and other issues over time 5 . Furthermore, it has been discovered that the usage of these down‐the‐drain items and the release of chemicals pressurizes the freshwater environment, resulting in a detrimental ecotoxicological impact 6 .…”

Section: Introductionmentioning

confidence: 99%

“…Some of the chemicals routinely employed in formulations have been found to cause molecular harm to cells, resulting in genetic alterations and other issues over time. 5 Furthermore, it has been discovered that the usage of these downthe-drain items and the release of chemicals pressurizes the freshwater environment, resulting in a detrimental ecotoxicological impact. 6 Easy commercialization, reasonable price, and an ever increasing market made this a popular choice among the buyer.…”

Section: Introductionmentioning

confidence: 99%

Development of EPS‐rich herbal shampoo base fermented using Cyclea peltata leaf powder and Lactobacillus plantarum

Devitheertha

Satheesh

Pradeep

et al. 2022

J of Cosmetic Dermatology

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Background Studies show that the extensive use of chemical shampoos has a negative impact on health. Given the recent trends, the use of herbal shampoos is gaining importance. Cyclea peltata is a common plant in Kerala's coastal region that has traditionally been used to aid in wound healing, allergy relief, and hair and scalp improvement. Greenly produced silver nanoparticles made from plant sources have a wide range of medical applications. Objective The main objective is to optimize the base of the shampoo with better solid content by OFAT studies and characterize the silver nanoparticles synthesized using post optimized shampoo base. Method Cyclea peltata leaves were fermented with Lactobacillus plantarum for the shampoo formulation, substituting the chemical basis gelatin with an herbal fermented foundation. Silver nanoparticles combined with an herbal formulation should be as safe as herbal shampoo while also being as effective as chemical shampoo. Variation of concentration of Cyclea peltata leaf powder, variation of sucrose concentration, variation of concentration of yeast extract, variation of L. plantarum inoculum, variation of temperature, variation of agitation speed, and variation of time were all studied using the OFAT (One Factor At a Time) method. Silver nanoparticles synthesized using post optimized shampoo bases were also characterized by particle size, zeta potential, and FTIR analysis in order to better understand their properties. Results The results clearly indicated that all the six factors had a significant effect on the growth and production of EPS. The pH considered for the shampoo base is above 5 so as to maintain the acidic mantle of the scalp. Green synthesized silver nanoparticles from post optimized shampoo base were obtained within 17th hour of incubation, with single surface plasmon resonance at 420 nm. Nanoparticles showed a peak at −11.6 mv of zeta potential which means that the particles are less agglomerative and stable. Similar groups were seen in ftir spectrum of fermented silver nanoparticles and the plant extract which confirmed the capping of nanoparticles with plant phytochemicals. Conclusion The study successfully prepared and characterized green synthesized silver nanoparticles from post optimized shampoo base and also optimized the shampoo base based on the EPS production. Characterization of the silver nanoparticles found that the nanoparticles synthesized were stable, less agglomerative, and had several useful components present in it.

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Isolation and Characterization of Malasezzia Species from Dandruff Samples and Determination of its Sensitivity Towards Antifungal Agents

Cited by 9 publications

References 12 publications

Antidandruff property of <i>Psidium guajava</i> leaf extracts

Antidandruff property of <i>Psidium guajava</i> leaf extracts

Dandruff Yang Disebabkan Jamur Dan Faktor Risiko Kejadian Pada Mahasiswa Teknologi Laboratorium Medis Poltekkes Kemenkes Jayapura

Development of EPS‐rich herbal shampoo base fermented using Cyclea peltata leaf powder and Lactobacillus plantarum

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