Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)

Abstract

ABSTRACT. Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus ranged from 3 to 23. The observed and expected heterozygosities per locus were 0.033-0.773 and 0.138-0.924, respectively. These microsatellite loci will facilitate the studies on genetic variation, mating system, and gene flow of Q. fabri.

Genetic diversity and population structure of Quercus fabri Hance in China revealed by genotyping‐by‐sequencing

“…The results also indicate that the genetic variation of native oak populations mainly exists within the population, the level of genetic variation among the populations is lower, and the gene exchange between the populations is frequent; compared with foreign oaks, the genetic diversity of some native oaks in China is lower (Greef, Triest, Cuyper, & Slycken, 1998;Hamrick, Godt, & Sherman-Broles, 1992;Kremer & Petit, 1993;Li et al, 2003;Wang, Hu, & Zhou, 2005). For Q. fabri, the latest related research is to develop 12 polymorphic and 2 monomorphic microsatellite loci, and the genetic diversity of Q. fabri has not been studied in depth (Xiao, Chen, Bao, Wang, & Li, 2016).…”