Isolation and characterization of microsatellite markers in two species of the neotropical Epicharis (Hymenoptera, Apidae, Centridini) genus and cross-amplification in related species

Souza-Shibatta, Lenice; Ricardo, Paulo Cseri; Pina, Welber da Costa; Flaresso-Neto, Vilmar; Freiria, Gabriele Antico; Kotelok-Diniz, Thais; Gaglianone, Maria Cristina; Arias, Maria Cristina; Sofia, Silvia Helena

doi:10.1007/s11033-020-06076-0

Mol Biol Rep

2021

DOI: 10.1007/s11033-020-06076-0

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Isolation and characterization of microsatellite markers in two species of the neotropical Epicharis (Hymenoptera, Apidae, Centridini) genus and cross-amplification in related species

Lenice Souza-Shibatta

Paulo Cseri Ricardo

Welber da Costa Pina

et al.

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2024

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Cited by 2 publications

References 15 publications

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The genetic status and rescue measure for a geographically isolated population of Amur tigers

Ning,

Liu,

et al. 2024

Sci Rep

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The Amur tiger is currently confronted with challenges of anthropogenic development, leading to its population becoming fragmented into two geographically isolated groups: smaller and larger ones. Small and isolated populations frequently face a greater extinction risk, yet the small tiger population’s genetic status and survival potential have not been assessed. Here, a total of 210 samples of suspected Amur tiger feces were collected from this small population, and the genetic background and population survival potentials were assessed by using 14 microsatellite loci. Our results demonstrated that the mean number of alleles in all loci was 3.7 and expected heterozygosity was 0.6, indicating a comparatively lower level of population genetic diversity compared to previously reported studies on other subspecies. The genetic estimates of effective population size (Ne) and the Ne/N ratio were merely 7.6 and 0.152, respectively, representing lower values in comparison to the Amur tiger population in Sikhote-Alin (the larger group). However, multiple methods have indicated the possibility of genetic divergence within our isolated population under study. Meanwhile, the maximum kinship recorded was 0.441, and the mean inbreeding coefficient stood at 0.0868, both of which are higher than those observed in other endangered species, such as the African lion and the grey wolf. Additionally, we have identified a significant risk of future extinction if the lethal equivalents were to reach 6.26, which is higher than that of other large carnivores. Further, our simulation results indicated that an increase in the number of breeding females would enhance the prospects of this population. In summary, our findings provide a critical theoretical basis for further bailout strategies concerning Amur tigers.

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The genetic status and rescue measure for a geographically isolated population of Amur tigers

Ning,

Liu,

et al. 2024

Sci Rep

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Genetic Diversity of Chinese Longsnout Catfish (Leiocassis longirostris) in Four Farmed Populations Based on 20 New Microsatellite DNA Markers

et al. 2022

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Freshwater aquaculture has a long and vibrant tradition in China. The Chinese longsnout catfish (Leiocassis longirostris) is a popular economic freshwater fish native to China. Understanding the genetic structure of L. longirostris populations is important for ensuring the efficacy of management practices and the sustainability of future increases in production. In this study, we used Illumina sequencing technology to isolate 20 novel polymorphic microsatellites from the genome of L. longirostris. These microsatellites were used to analyze the genetic diversity of 240 L. longrostris individuals from four populations. Genetic diversity parameters (NA, HO, HE, I, PIC, and FST) of the four farmed L. longirostris populations were analyzed. The level of genetic differentiation among the four farmed L. longirostris populations (inferred by pairwise comparisons of FST values) was low, but the genetic diversity of these populations was high, indicating that they still provide useful sources of genetic variation that could aid in breeding efforts. The STRUCTURE and ADMIXTURE analyses indicated that admixture might be occurring in the four L. longirostris populations, especially between the MS and YB populations. Understanding the genetic diversity of farmed L. longirostris populations and inbreeding prevention could greatly aid in breeding and production. These newly isolated microsatellite markers and the high genetic diversity of L. longirostris populations in the main breeding areas have important implications for the breeding and stock management of L. longirostris.

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Isolation and characterization of microsatellite markers in two species of the neotropical Epicharis (Hymenoptera, Apidae, Centridini) genus and cross-amplification in related species

Cited by 2 publications

References 15 publications

The genetic status and rescue measure for a geographically isolated population of Amur tigers

The genetic status and rescue measure for a geographically isolated population of Amur tigers

Genetic Diversity of Chinese Longsnout Catfish (Leiocassis longirostris) in Four Farmed Populations Based on 20 New Microsatellite DNA Markers

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