Isolation and characterization of microsatellite loci in Paspalum notatum Flüggé (Poaceae)

Cidade, Fernanda Witt; Souza, Anete Pereira de; Batista, L. A. R.; Dall'Agnol, Miguel; Zucchi, Maria Imaculada; Jungmann, L.; Souza, Anete Pereira de

doi:10.1007/s10592-009-9872-6

Cited by 20 publications

(38 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…notatum using the method described by Billotte et al [69]. The steps for obtaining the clones, sequencing and analysis, the determination of the criteria for selecting the microsatellites, the primer design and the amplification were conducted according to previously described methodology [58]. For some loci, a touchdown protocol was used, as previously described [70].…”

Section: Methodsmentioning

confidence: 99%

Genetic variation in polyploid forage grass: Assessing the molecular genetic variability in the Paspalumgenus

et al. 2013

View full text Add to dashboard Cite

BackgroundPaspalum (Poaceae) is an important genus of the tribe Paniceae, which includes several species of economic importance for foraging, turf and ornamental purposes, and has a complex taxonomical classification. Because of the widespread interest in several species of this genus, many accessions have been conserved in germplasm banks and distributed throughout various countries around the world, mainly for the purposes of cultivar development and cytogenetic studies. Correct identification of germplasms and quantification of their variability are necessary for the proper development of conservation and breeding programs. Evaluation of microsatellite markers in different species of Paspalum conserved in a germplasm bank allowed assessment of the genetic differences among them and assisted in their proper botanical classification.ResultsSeventeen new polymorphic microsatellites were developed for Paspalum atratum Swallen and Paspalum notatum Flüggé, twelve of which were transferred to 35 Paspalum species and used to evaluate their variability. Variable degrees of polymorphism were observed within the species. Based on distance-based methods and a Bayesian clustering approach, the accessions were divided into three main species groups, two of which corresponded to the previously described Plicatula and Notata Paspalum groups. In more accurate analyses of P. notatum accessions, the genetic variation that was evaluated used thirty simple sequence repeat (SSR) loci and revealed seven distinct genetic groups and a correspondence of these groups to the three botanical varieties of the species (P. notatum var. notatum, P. notatum var. saurae and P. notatum var. latiflorum).ConclusionsThe molecular genetic approach employed in this study was able to distinguish many of the different taxa examined, except for species that belong to the Plicatula group, which has historically been recognized as a highly complex group. Our molecular genetic approach represents a valuable tool for species identification in the initial assessment of germplasm as well as for characterization, conservation and successful species hybridization.

show abstract

Section: Methodsmentioning

confidence: 99%

Genetic variation in polyploid forage grass: Assessing the molecular genetic variability in the Paspalumgenus

et al. 2013

View full text Add to dashboard Cite

show abstract

“…They are better than the others since they do not require prior information of the DNA sequence, have low development costs, and have high transferability to other plant species, and generate a greater number of polymorphic fragments (Barth et al, 2002;Brandão et al, 2011). ISSR markers have been found to be efficient in several studies on genetic population variability (Cidade et al, 2009;Rossi et. al., 2009;Brandão et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Population structure and genetic diversity in natural populations of Theobroma speciosum Willd. Ex Spreng (Malvaceae)

Giustina¹,

Luz

Vieira³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The genus Theobroma found in the Amazon region is composed of 22 species, including Theobroma speciosum, better known as cacauí. These species are constantly threatened by forest fragmentation caused by human activities and require conservation strategies and management aimed at preserving them in their natural environments. The main objective of this study was to analyze the population structure and genetic diversity within and between natural populations of T. speciosum by using ISSR molecular markers to understand the population structure of the species. Four natural populations belonging to the Amazon rainforest (BAC, CRO, FLA, and PNA), located in the State of Mato Grosso, were selected. Amplification reactions were performed using 15 ISSR primers. A total of 101 loci 3511 Genetic diversity in Theobroma speciosum©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 3510-3519 (2014) were found, of which 54.46% were polymorphic at the species level. The BAC population showed higher genetic diversity (H = 0.095 and I = 0.144) and higher percentage of polymorphism (28.71%). The populations showed an F ST value of 0.604, indicating marked genetic differentiation. The highest genetic variation was found between populations. Gene flow was low between populations, indicating genetic isolation between populations.

show abstract

“…These markers stand out because they do not need prior information of the DNA sequence, have low development costs, present laboratory procedures with good rates of transferability for other plant species (Bhart et al, 2002), and generate a large number of polymorphic fragments in spite of the low-cost of the technique (Brandão et al, 2011). ISSR markers have proven to be efficient in the many studies on genetic variability of populations that have used them (Cidade et al, 2009;Rossi et al, 2009;Brandão et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in natural populations of Theobroma subincanum Mart. in the Brazilian Amazon

Rivas¹,

Giustina²,

Luz³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The genus Theobroma, recently reclassified in the family Malvaceae, comprises some species with high economic potential, including the cupuí, Theobroma subincanum Mart., which has not yet been domesticated, and whose genetics and population structure are mostly unknown. This study aimed to assess the population structure and genetic diversity in natural populations of T. subincanum Mart., using inter-simple sequence repeat (ISSR) markers. A total of 59 individuals were sampled in three geographically separate populations, CFA, CMN, and CPT. Nei's genetic distance was estimated to characterize populations with the use of 13 polymorphic primers. The analysis of 4999 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 4998-5006 (2013) Genetic diversity in Theobroma subincana Mart. molecular variance revealed that the variability between populations (51.71%) was higher than that within populations (48.29%). Among the three populations, CPT showed the highest diversity index and percentage of polymorphism. The ISSR molecular markers were efficient and presented sufficient polymorphism to estimate genetic diversity in populations of T. subincanum Mart.

show abstract

Isolation and characterization of microsatellite loci in Paspalum notatum Flüggé (Poaceae)

Cited by 20 publications

References 7 publications

Genetic variation in polyploid forage grass: Assessing the molecular genetic variability in the Paspalumgenus

Genetic variation in polyploid forage grass: Assessing the molecular genetic variability in the Paspalumgenus

Population structure and genetic diversity in natural populations of Theobroma speciosum Willd. Ex Spreng (Malvaceae)

Genetic diversity in natural populations of Theobroma subincanum Mart. in the Brazilian Amazon

Contact Info

Product

Resources

About