Isolation and characterization of microsatellite loci from blue-footed boobies (Sula nebouxii)

Faircloth, Brant C.; Ramos, Alejandra; Drummond, Hugh; Gowaty, Patricia Adair

doi:10.1007/s12686-009-9038-3

Cited by 8 publications

(6 citation statements)

References 20 publications

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“…Therefore, we used a number of published markers developed for related booby species that showed sufficient levels of polymorphism (Table ) (Faircloth et al. ; Morris‐Pocock et al. ; Taylor et al.…”

Section: Methodsmentioning

confidence: 99%

“…Microsatellite primers specific for Nazca boobies were not available. Therefore, we used a number of published markers developed for related booby species that showed sufficient levels of polymorphism ( Table 1 ) ( Faircloth et al 2009 ; Morris-Pocock et al 2010a ; Taylor et al 2010a ). Twenty-five primer pairs were tested, and 17 were rejected due to monomorphism or poor amplification.…”

Section: Methodsmentioning

confidence: 99%

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Philopatry drives genetic differentiation in an island archipelago: comparative population genetics of Galapagos Nazca boobies (Sula granti) and great frigatebirds (Fregata minor)

Levin

Parker

2012

Ecology and Evolution

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Seabirds are considered highly mobile, able to fly great distances with few apparent barriers to dispersal. However, it is often the case that seabird populations exhibit strong population genetic structure despite their potential vagility. Here we show that Galapagos Nazca booby (Sula granti) populations are substantially differentiated, even within the small geographic scale of this archipelago. On the other hand, Galapagos great frigatebird (Fregata minor) populations do not show any genetic structure. We characterized the genetic differentiation by sampling five colonies of both species in the Galapagos archipelago and analyzing eight microsatellite loci and three mitochondrial genes. Using an F-statistic approach on the multilocus data, we found significant differentiation between nearly all island pairs of Nazca booby populations and a Bayesian clustering analysis provided support for three distinct genetic clusters. Mitochondrial DNA showed less differentiation of Nazca booby colonies; only Nazca boobies from the island of Darwin were significantly differentiated from individuals throughout the rest of the archipelago. Great frigatebird populations showed little to no evidence for genetic differentiation at the same scale. Only two island pairs (Darwin – Wolf, N. Seymour – Wolf) were significantly differentiated using the multilocus data, and only two island pairs had statistically significant φST values (N. Seymour – Darwin, N. Seymour – Wolf) according to the mitochondrial data. There was no significant pattern of isolation by distance for either species calculated using both markers. Seven of the ten Nazca booby migration rates calculated between island pairs were in the south or southeast to north or northwest direction. The population differentiation found among Galapagos Nazca booby colonies, but not great frigatebird colonies, is most likely due to differences in natal and breeding philopatry.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Philopatry drives genetic differentiation in an island archipelago: comparative population genetics of Galapagos Nazca boobies (Sula granti) and great frigatebirds (Fregata minor)

Levin

Parker

2012

Ecology and Evolution

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“…Thirteen loci were combined for amplification in five multiplexes and five loci were amplified in single locus PCRs ([1] Sn2B‐68, Sv2A‐53 Ss1B‐100; [2] Ss1B‐88, Sn2B‐83, Sv2A‐47; [3] BOOB‐RM4‐C03, BOOB‐RM4F11, BOOB‐RM4‐G03; [4] BOOB‐RM4‐D07, BOOB‐RM4‐E10; [5] BOOB‐ RM2‐F07, BOOB‐RM4‐E03; [6] Ss2B‐138; [7] BOOB‐RM3‐F11; [8] BOOB‐RM4‐b3; [9] BOOB‐RM3‐D07; [10] Sn2A‐36). Thirteen of the primer pairs were designed from blue‐footed booby genomic libraries (Faircloth et al 2009, Taylor et al 2010a), two were designed from a Peruvian booby genomic library (Taylor et al 2010a), and three were designed from a red‐footed booby Sula sula genomic library (loci Ss1b‐88, Ss1b‐100, Ss2b‐138)(Molecular Ecology Resources Primer Development Consortium 2010). Primers for locus Ss1B‐88 and locus Ss1B‐100 were redesigned for blue‐footed boobies (Taylor et al 2011a).…”

Section: Methodsmentioning

confidence: 99%

Evidence for strong assortative mating, limited gene flow, and strong differentiation across the blue‐footed/Peruvian booby hybrid zone in northern Peru

Taylor

Anderson

and

et al. 2012

Journal of Avian Biology

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Hybrid zones represent natural laboratories in which the processes of divergence and genetic isolation can be examined. The generation and maintenance of a hybrid zone requires mispairing and successful reproduction between organisms that differ in one or more heritable traits. Understanding the dynamics of hybridization between two species requires an understanding of the extent to which they have diverged genetically, the frequency of misparing and hybrid production, and the extent of introgression. Three hundred and twenty one blue‐footed Sula nebouxii and Peruvian S. variegata boobies from the eastern tropical Pacific Ocean were analyzed using 19 putatively neutral genetic markers to evaluate interspecific differentiation, to classify morphological hybrids using Bayesian assignments, and to characterize hybridization using cline theory and Bayesian assignments. The species were well differentiated at mitochondrial and nuclear microsatellites, the hybrid zone was bimodal (contained a high frequency of each parental species but a low frequency of hybrids), and morphologically intermediate individuals were most likely F1 hybrids resulting from mating between female Peruvian boobies and male blue‐footed boobies. Clines in allele frequency could be constrained to share a common geographic centre but could not be constrained to share a common width. Peruvian and blue‐footed boobies hybridize infrequently, potentially due to strong premating reproductive isolation; however, backcrossing appears to facilitate introgression from blue‐footed to Peruvian boobies in this hybrid system.

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“…In that year, all nests in the study plot (a 8,450-m 10 days. We extracted DNA with illustra blood genomicPrep Mini Spin kits from GE Healthcare and analyzed paternity using ten blue-footed booby microsatellite loci that had 3 to 22 alleles (Faircloth et al 2009). All of these loci were checked for null alleles and were in Hardy-Weinberg equilibrium (Raymond and Rousset 1995).…”

Section: Samplingmentioning

confidence: 99%

Interactive effects of male and female age on extra-pair paternity in a socially monogamous seabird

Ramos

Nunziata

Lance

et al. 2014

Behav Ecol Sociobiol

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Females sometimes obtain older sires for their offspring through extra-pair interactions, but how female age influences paternity is largely unexplored and interactive effects across the age span of both sexes have not been analyzed. To test whether female choice of sire age varies with female age in the blue-footed booby (Sula nebouxii), we examined associations between ages of both partners and the probability of extrapair paternity (EPP) in 350 broods of parents up to 22 years old in a single breeding season. Extra-pair paternity enables a female to select an alternative sire for her offspring and could function to avoid or achieve particular combinations of parental ages. A male age×female age interaction revealed that in young females (≤4 years), EPP decreased with increasing age of the social partner, whereas in old females (≥8 years), it increased. Moreover, sires of extra-pair (EP) chicks of young females paired to young males were on average 6.33 years older than the females' social partners. Since female boobies control copulatory access, this pattern could imply that young females choose old sires for their proven genetic quality and that old females avoid very old males because matings with them may risk infertility or genetic defects in offspring. Taking female age into account and observing across the whole age span may be necessary for understanding female age-based mate choice.

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Isolation and characterization of microsatellite loci from blue-footed boobies (Sula nebouxii)

Cited by 8 publications

References 20 publications

Philopatry drives genetic differentiation in an island archipelago: comparative population genetics of Galapagos Nazca boobies (Sula granti) and great frigatebirds (Fregata minor)

Philopatry drives genetic differentiation in an island archipelago: comparative population genetics of Galapagos Nazca boobies (Sula granti) and great frigatebirds (Fregata minor)

Evidence for strong assortative mating, limited gene flow, and strong differentiation across the blue‐footed/Peruvian booby hybrid zone in northern Peru

Interactive effects of male and female age on extra-pair paternity in a socially monogamous seabird

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