Isolation and characterization of microsatellites from Scaptotrigona xanthotricha (Apidae, Meliponini): a stingless bee in the Brazilian Atlantic rainforest

Duarte, Olívia Maria Pereira; Gaiotto, Fernanda Amato; Souza, Anete Pereira de; Mori, Gustavo Maruyama; Costa, Marco A. Dalla

doi:10.1007/s13592-011-0109-6

Cited by 7 publications

(9 citation statements)

References 8 publications

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“…Low genetic variability in native stingless bees is also documented in studies using molecular markers such as RAPD (Tavares et al, 2001), mitochondrial polymorphism (Brito et al, 2013), ISSR (Inter Single Sequence Repeats) (Nascimento et al, 2010;Miranda et al, 2012), and microsatellites (Francisco et al, 2006;Tavares et al, 2007;Carvalho-Zilse et al, 2009;Francini et al, 2009;Alves et al, 2011;Duarte et al, 2011).…”

Section: Discussionmentioning

confidence: 89%

Genetic Variability of Stingless Bees Melipona mondury Smith and Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae) from a Meliponary

Koser¹,

Francisco²,

Moretto³

2014

Sociobiology

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Section: Discussionmentioning

confidence: 89%

Genetic Variability of Stingless Bees Melipona mondury Smith and Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae) from a Meliponary

Koser¹,

Francisco²,

Moretto³

2014

Sociobiology

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“…They were preserved in absolute ethanol for genetic analysis. The DNA was extracted using the Chelex method and males were genotyped at six microsatellite loci, T1, T3, T4 and T8 (Paxton et al 1999), and Sxant06 and Sxant18 (Duarte et al 2011). Microsatellite amplification and visualization were done as described previously by Francisco et al (2011).…”

Section: Introductionmentioning

confidence: 99%

Diploid males of Scaptotrigona depilis are able to join reproductive aggregations (Apidae, Meliponini)

Vollet-Neto

Santos

Santiago

et al. 2015

JHR

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The sex determination system in the eusocial stingless bees (Apidae, Meliponini) is based on the combination of alleles at the complementary sex determination (CSD) locus. In this system, males are haploid and females are diploid. However, diploid males can develop from fertilized eggs when they are homozygous at single or multiple sex loci. The production of such males can negatively affect population viability, since they are usually infertile or inviable. Moreover, when they are viable but infertile, or siring sterile triploid offspring, this could cause another load on the population, leading the fertilized offspring of other females to be only haploid males or triploid sterile daughters. In this context, our aim was to verify whether diploid males of the stingless bee Scaptotrigonadepilis do in fact join reproductive aggregations. We showed that of 360 marked males from two different colonies, five were participating in a reproductive aggregation ca. 20 meters from their natal colonies. Using microsatellites markers, it was confirmed that three of these five males were diploid. They were captured in the mating aggregations when they were 15 to 20 days old. Further research is necessary to determine the mating success of stingless bee diploid males under natural conditions and to determine their impact on stingless bee population extinction risks.

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Section: Introductionmentioning

confidence: 99%

Diploid males of Scaptotrigona depilis are able to join reproductive aggregations (Apidae, Meliponini)

Vollet-Neto¹,

Santos²,

Santiago³

et al. 2015

JHR

View full text Add to dashboard Cite

The sex determination system in the eusocial stingless bees (Apidae, Meliponini) is based on the combination of alleles at the complementary sex determination (CSD) locus. In this system, males are haploid and females are diploid. However, diploid males can develop from fertilized eggs when they are homozygous at single or multiple sex loci. The production of such males can negatively affect population viability, since they are usually infertile or inviable. Moreover, when they are viable but infertile, or siring sterile triploid offspring, this could cause another load on the population, leading the fertilized offspring of other females to be only haploid males or triploid sterile daughters. In this context, our aim was to verify whether diploid males of the stingless bee Scaptotrigona depilis do in fact join reproductive aggregations. We showed that of 360 marked males from two different colonies, five were participating in a reproductive aggregation ca. 20 meters from their natal colonies. Using microsatellites markers, it was confirmed that three of these five males were diploid. They were captured in the mating aggregations when they were 15 to 20 days old. Further research is necessary to determine the mating success of stingless bee diploid males under natural conditions and to determine their impact on stingless bee population extinction risks.

show abstract

Isolation and characterization of microsatellites from Scaptotrigona xanthotricha (Apidae, Meliponini): a stingless bee in the Brazilian Atlantic rainforest

Abstract: International audienc

Cited by 7 publications

References 8 publications

Genetic Variability of Stingless Bees Melipona mondury Smith and Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae) from a Meliponary

Genetic Variability of Stingless Bees Melipona mondury Smith and Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae) from a Meliponary

Diploid males of Scaptotrigona depilis are able to join reproductive aggregations (Apidae, Meliponini)

Diploid males of Scaptotrigona depilis are able to join reproductive aggregations (Apidae, Meliponini)

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