Isolation and Characterization of Microsatellites in the Green Alga Ulva compressa (Chlorophyta)

Kagami, Yayoi; Arai, Tatsushi; Mogi, Yuko; Kuwano, Kazuyoshi; Kawano, Shigeyuki

doi:10.1508/cytologia.73.387

Cited by 7 publications

(7 citation statements)

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“…Such studies will preclude the occurrence of another type of physiological isolation if hybrids capable of further reproduction are obtained. This type of verification may be complemented by the use of markers that are more sensitive to the differences between the taxa, such as micro-satellite sequences and DNA -fingerprinting techniques (ISSR and AFLP) which have been successfully used in phylogenetic studies of closely related species (Kagami et al 2008, Kostamo et al 2008, hydrides (Gupta et al 2015) and Baltic populations of Ulva intestinalis and U. compressa (Alström-Rapaport & Leskinen 2002, Alström-Rapaport et al 2010). …”

Section: Discussionmentioning

confidence: 99%

Differentiation of inland Ulva flexuosa Wulfen (Ulvaceae, Chlorophyta) from Western Poland

Rybak

Czerwoniec²

2015

Oceanological and Hydrobiological Studies

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I n t e r n a t i o n a l J o u r n a l o f O c e a n o g r a p h y a n d H y d r o b i o l o g y AbstractThe macroalgae representing the cosmopolitan Ulva flexuosa Wulfen species have been found in inland water ecosystems of Europe since the mid-1800s. The presence of the Ulva genus in waters of inland salt marshes was explained by high salinity of water in such systems. However, Ulva flexuosa thalli were found in lakes and rivers having no supply from strongly mineralized sources, either of natural or anthropogenic origin. The study focused only on populations of Ulva flexuosa subsp. pilifera (Kűtz.) M.J. Wynne and Ulva flexuosa subsp. paradoxa (C. Agardh) M.J. Wynne occurring in inland ecosystems isolated from seawater. The differentiation of inland U. flexuosa subsp. pilifera and subspecies paradoxa was assessed by using classical morphological data and molecular techniques. We found that only two subspecies of Ulva flexuosa Wulfen occur in inland water ecosystems of Poland. On the basis of the analysis combining morphological features with the ITS region and rbcL gene sequences, a small degree of differentiation of the two inland taxa was demonstrated. These two subspecies have high differentiation of the habitat niches. U. flexuosa subsp. paradoxa settled in habitats featuring high salinity levels, and the second subspecies pilifera occurred only in fresh waters.

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Section: Discussionmentioning

confidence: 99%

Differentiation of inland Ulva flexuosa Wulfen (Ulvaceae, Chlorophyta) from Western Poland

Rybak

Czerwoniec²

2015

Oceanological and Hydrobiological Studies

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“…This technique will make crossing experiments easier and more reproducible. As microsatellite markers have recently been developed for U. compressa (Kagami et al 2008a), these markers can be used to assess the success of crossing experiments in this species. These reliable techniques for the crossing experiments would accelerate studies on the genetics and the mechanism of mating.…”

Section: Discussionmentioning

confidence: 99%

DURATIONS OF GAMETE MOTILITY AND CONJUGATION ABILITY OF ULVA COMPRESSA (ULVOPHYCEAE)¹

Kuwano¹,

Hashioka

Nishihara

et al. 2012

Journal of Phycology

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The present study was designed to develop a technique for crossing and to gain insight into how sexual reproduction contributes to the maintenance of local populations of Ulva compressa L. To examine the durations of gamete motility and conjugation ability, freshly released gametes were incubated for various periods of time prior to mixing both mating types. The conjugation ability of the gametes gradually declined after being released from the thalli when the gametes were incubated without mixing with the opposite mating type. The ability to conjugate decreased by half after 6 h, although most of the gametes remained motile. The gametes released 4 h later had the same level of conjugation ability when mixed immediately after releasing. When the mature thalli were wrapped in a moist paper towel to prevent gametes from being released, the gametes were preservable for 7 h without a significant decrease in their conjugation ability. Conjugation occurred soon after mixing gametes of both mating types and reached a plateau after 30 s. However, conjugation rates did not exceed a rate of ∼70%, even though freshly released gametes were used. Interestingly, a portion of the gametes newly conjugated 30 min after mixing both mating types, and conjugation rates reached a second plateau at ∼90%. Gametes with delayed conjugation are provided some period of time that allows them to be transported away and increases their chances of mating with more distant populations, thus contributing to the maintenance of genetic variation.

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“…A pair of mt and mt + cultures of U. compressa (MGEC-2 and MGEC-1, respectively) was collected from the Konokawa Coast, Iyo, Ehime, Japan (33 42′N, 132 39′E) (Kagami et al 2005, Kagami et al 2008a, Kagami et al 2008b, Mogi et al 2008. The strains are maintained in the culture collection of Nagasaki University (Nagasaki, Japan).…”

Section: Algal Strains Culture and Zygote Formationmentioning

confidence: 99%

HAP2/GCS1 Is Involved in the Sexual Reproduction System of the Marine Macroalga Ulva compressa (Ulvales, Chlorophyta)

et al. 2014

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Summary Gamete fusion or fertilization is one of the most important steps in sexual reproduction. HAP2/GCS1 is a potential gamete membrane fusion component. We analyzed whole genome sequences of two strains with opposite mating types (mt and mt + ) in the marine macroalga Ulva compressa and found that identical genomic regions homologous with HAP2/GCS1 existed in their draft genome sequences. Although an RNA-Seq analysis revealed that a variety of transcripts was expressed from the two regions containing HAP2/GCS1 in both the gamete and gametophyte of the mt and mt + genotypes, a putative transcript coding a protein containing a HAP2-GCS1 domain was identified only in the mt gamete. Immunofluorescence observations using an antibody against the putative HAP2/GCS1 demonstrated that the HAP2/GCS1 translational products localized to the cellular surface between the two flagella at a potential site for the mt mating structure. The antibody inhibited mating of the mt gamete with the mt + gamete in U. compressa. Our data suggest that HAP2/GCS1 functions in the mt gamete and plays a role in gamete fusion. Moreover, the specific function of HAP2/GCS1 in the mt mating type may be regulated by an alternative splicing mechanism in U. compressa.

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Isolation and Characterization of Microsatellites in the Green Alga Ulva compressa (Chlorophyta)

Cited by 7 publications

References 8 publications

Differentiation of inland Ulva flexuosa Wulfen (Ulvaceae, Chlorophyta) from Western Poland

Differentiation of inland Ulva flexuosa Wulfen (Ulvaceae, Chlorophyta) from Western Poland

DURATIONS OF GAMETE MOTILITY AND CONJUGATION ABILITY OF ULVA COMPRESSA (ULVOPHYCEAE)¹

HAP2/GCS1 Is Involved in the Sexual Reproduction System of the Marine Macroalga Ulva compressa (Ulvales, Chlorophyta)

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Isolation and Characterization of Microsatellites in the Green Alga Ulva compressa (Chlorophyta)

Cited by 7 publications

References 8 publications

Differentiation of inland Ulva flexuosa Wulfen (Ulvaceae, Chlorophyta) from Western Poland

Differentiation of inland Ulva flexuosa Wulfen (Ulvaceae, Chlorophyta) from Western Poland

DURATIONS OF GAMETE MOTILITY AND CONJUGATION ABILITY OF ULVA COMPRESSA (ULVOPHYCEAE)1

HAP2/GCS1 Is Involved in the Sexual Reproduction System of the Marine Macroalga Ulva compressa (Ulvales, Chlorophyta)

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DURATIONS OF GAMETE MOTILITY AND CONJUGATION ABILITY OF ULVA COMPRESSA (ULVOPHYCEAE)¹