Isolation and characterization of microsatellites in yellow perch (Perca flavescens)

Abstract

A total of 45 microsatellite loci from yellow perch, Perca flavescens, were isolated and characterized. Among the 45 microsatellite loci, 32 had more than two alleles. A wild population of P. flavescens (n = 48) was used to examine the allele range of the microsatellite loci. Mendelian inheritance of alleles was confirmed by examining the amplified products in pair‐mated families. The number of alleles for the 32 polymorphic loci varied from two to 16, and observed heterozygosity ranged between 0.024 (YP79) and 0.979 (YP60). Cross‐species polymorphic amplification in four other Percidae species was successful for 22 loci.

Genetic diversity in natural and introduced island populations of koalas in Queensland

“…This family exhibited a transmission ratio distortion in the form of heterozygote excess. This is a common occurrence in inheritance studies (Reece et al 2004;Karlsson et al 2007;Li et al 2007;Guzinski et al 2008). This could be an incident of segregation distortion, a ubiquitous phenomenon, which is characterized by a deviation from expected Mendelian ratios due to high heterozygosity (Aparicio et al 2010;Liu et al 2010).…”

Physiological and proteomic evidences that domestication process differentially modulates the immune status of juvenile Eurasian perch (Perca fluviatilis) under chronic confinement stress

“…Based on preliminary tests (data not shown), eight polymorphic microsatellite loci were selected and amplified independently: Pfla L2, Pfla L4, Pfla L6, Pfla L9 [43], YP60, YP68, YP78 and YP111 [44].…”

Patterns of genetic structure of Eurasian perch (Perca fluviatilis L.) in Lake Geneva at the end of the spawning season

“…Twelve microsatellite markers were used: PflaL1, PflaL2, PflaL4, PflaL6, PflaL9, PflaL10 (Leclerc et al, 2000), and YP60, YP78, YP111 (Li et al, 2007) that were previously developed for yellow perch (Perca flavescens); SviL7 (Wirth et al, 1999), and Svi17, Svi18 (Borer et al, 1999) that were developed for walleye (Sander vitreus). For each sample, PCR and genotyping were conducted in four multiplexes using the QIAGEN Multiplex reactions PCR Plus Kit and fluorescently labeled primers (VIC, NED, 6-FAM and PET).…”