Isolation and characterization of multi-potential<i>Rhizobium</i>strain ND2 and its plant growth-promoting activities under Cr(VI) stress

Karthik, Chinnannan; Oves, Mohammad; Sathya, Kumar; Ramkumar, Vijayan Sri; Padikasan, Indra Arulselvi

doi:10.1080/03650340.2016.1261116

Cited by 34 publications

(19 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Changes in temperatures, metal toxicity, pH and soil salinity are among the main factors restricting symbiotic nitrogen fixation in legume-rhizobia symbiosis and thus only those strains capable of tolerating the extreme conditions would survive ( Berrada et al, 2012 ). The production of exopolysaccharides (EPS) by most of the isolates in this study could indicate their versatility to withstand physiological stress due to high temperatures, metal toxicity, low soil pH and salinity ( Karthik et al, 2017 ). Soils in AEZ surrounding Mt.…”

Section: Discussionmentioning

confidence: 97%

“…Rhizobia bacteria play a significant role in provision of agricultural ecosystem services due to their ability to form symbiotic association with a wide range of leguminous plants that results in biological nitrogen fixation ( Orrell and Bennett, 2013 ). Some of the rhizobia strains are reported to enhance the production of phytohormones, mineral uptake and reduce toxic effects of metals, thereby, indirectly promoting plant growth and development ( Karthik et al, 2017 ) in polluted agricultural soils. Modern agriculture has shifted to the use of sustainable farming practices that are eco-friendly, efficient, and affordable to the resource-limited smallholder farmers.…”

Section: Introductionmentioning

confidence: 99%

“…Apart from ARDRA tools, other PCR-based techniques such as random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) have been used to evaluate the diversity of rhizobia ( Silva et al, 2012 ; Onyango et al, 2015 ; Boakye et al, 2016 ). In addition, the 16S rRNA and 16S-23S rRNA genes, which are highly conserved, have been sequenced to determine the taxonomic position of different rhizobia strains ( Rahmani et al, 2011 ; Adhikari et al, 2013 ; Karthik et al, 2017 ). Several rhizobia specific and universal primers have been used in amplification and sequencing and have produced distinguishable rhizobia phylogenies.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Characterization and Diversity of Rhizobium Isolated From Root Nodules of Mid-Altitude Climbing Bean (Phaseolus vulgaris L.) Varieties

et al. 2018

View full text Add to dashboard Cite

The increasing interest in the use of rhizobia as biofertilizers in smallholder agricultural farming systems of the Sub-Saharan Africa has prompted the identification of a large number of tropical rhizobia strains and led to studies on their diversity. Inoculants containing diverse strains of rhizobia have been developed for use as biofertilizers to promote soil fertility and symbiotic nitrogen fixation in legumes. In spite of this success, there is paucity of data on rhizobia diversity and genetic variation associated with the newly released and improved mid-altitude climbing (MAC) bean lines (Phaseolus vulgaris L.). In this study, 41 rhizobia isolates were obtained from the root nodules of MAC 13 and MAC 64 climbing beans grown in upper and lower midland agro-ecological zones of Eastern Kenya. Eastern Kenya was chosen because of its high production potential of diverse common bean cultivars. The rhizobia isolates were characterized phenotypically on the basis of colony morphology, growth and biochemical features. Rhizobia diversity from the different regions of Eastern Kenya was determined based on the amplified ribosomal DNA restriction analysis (ARDRA) of PCR amplified 16S rRNA genes using Msp I, EcoR I, and Hae III restriction enzymes. Notably, native rhizobia isolates were morphologically diverse and grouped into nine different morphotypes. Correspondingly, the analysis of molecular variance based on restriction digestion of 16S rRNA genes showed that the largest proportion of significant (p < 0.05) genetic variation was distributed within the rhizobia population (97.5%) than among rhizobia populations (1.5%) in the four agro-ecological zones. The high degree of morphological and genotypic diversity of rhizobia within Eastern Kenya shows that the region harbors novel rhizobia strains worth exploiting to obtain strains efficient in biological nitrogen fixation with P. vulgaris L. Genetic sequence analysis of the isolates and testing for their symbiotic properties should be carried out to ascertain their identity and functionality in diverse environments.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Characterization and Diversity of Rhizobium Isolated From Root Nodules of Mid-Altitude Climbing Bean (Phaseolus vulgaris L.) Varieties

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Rhizobia is a group of rhizospheric bacteria that establish nitrogen-fixing symbiosis with legumes, and so far no public or environmental health problems have been reported regarding their use as biofertilizers 13 , 14 . In addition to symbiotic nitrogen fixation, rhizobia have other PGP mechanisms, such as phosphate solubilisation and the production of siderophores and the phytohormone indole acetic acid (IAA) 9 , 13 , 15 – 22 . This suggests that these bacteria can also be used as a plant probiotic for non-legumes, a situation that has recently been reported for some cereals, fruits, and vegetables 9 , 13 , 15 , 16 , 19 – 21 , 23 – 26 .…”

Section: Introductionmentioning

confidence: 99%

Probiotic activities of Rhizobium laguerreae on growth and quality of spinach

Jiménez‐Gómez

Flores-Félix

García‐Fraile

et al. 2018

Sci Rep

View full text Add to dashboard Cite

The growing interest in a healthy lifestyle and in environmental protection is changing habits regarding food consumption and agricultural practices. Good agricultural practice is indispensable, particularly for raw vegetables, and can include the use of plant probiotic bacteria for the purpose of biofertilization. In this work we analysed the probiotic potential of the rhizobial strain PEPV40, identified as Rhizobium laguerreae through the analysis of the recA and atpD genes, on the growth of spinach plants. This strain presents several in vitro plant growth promotion mechanisms, such as phosphate solubilisation and the production of indole acetic acid and siderophores. The strain PEPV40 produces cellulose and forms biofilms on abiotic surfaces. GFP labelling of this strain showed that PEPV40 colonizes the roots of spinach plants, forming microcolonies typical of biofilm initiation. Inoculation with this strain significantly increases several vegetative parameters such as leaf number, size and weight, as well as chlorophyll and nitrogen contents. Therefore, our findings indicate, for the first time, that Rhizobium laguerreae is an excellent plant probiotic, which increases the yield and quality of spinach, a vegetable that is increasingly being consumed raw worldwide.

show abstract

“…Their resistance to metals is very similar to what it knows in general on Rhizobia. Tolerance to heavy metals could be exploited in the bioremediation trials aimed at improving the quality of soil contaminated with heavy metals sites [14,25].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Indigenous Rhizobia Strains Associated to Soybean [Glycine max (L.) Merrill] in Benin

Zoundji¹,

Houngnandan²,

Boko

et al. 2020

IJPSS

View full text Add to dashboard Cite

Legumes such as soybean establish symbiotic relation with nitrogen fixing bacteria such as Rhizobia. Nitrogen fixation via legume-rhizobium symbiosis is the most important source of Nitrogen in agro-ecosystems. But environmental stresses are important limiting factors for this process. Hence, the aim of this study is to evaluate the physiological characteristics and Plant Growth Promoting (PGP) properties of soybean rhizobia. A total of 28 Rhizobia strains obtained from soybean root nodules collected in from three Agro-Ecological Zones (zones 3, 4 and 5) producers of soybean in Benin were used. The physiological characteristics include utilization of carbon source, tolerance to temperature, salinity and pH, resistance to antibiotics and heavy metals. The PGP properties were relative to production of indole, hydrogen cyanide and ammonia and catalase test. The results revealed that, irrespective of their geographical regions, the 28 isolates were grouped into five Clusters. Most of them tolerated neutral to alkaline pH and high salt stress and 17% of them could grow at 40°C. Most of them showed resistance to heavy metal and antibiotics. These isolates tested were able to use a broad range of carbohydrates as sole source of carbon. Production of indole, hydrogen cyanide and ammoniac were respectively found on 56%, 41% and 44% of isolates but all isolates gave positive reaction to catalase test. These rhizobial isolates showing best physiological and PGP properties could be good candidates to establish a successful symbiosis with soybean under the variation of environmental conditions that prevail in Benin.

show abstract

Isolation and characterization of multi-potentialRhizobiumstrain ND2 and its plant growth-promoting activities under Cr(VI) stress

Cited by 34 publications

References 26 publications

Genetic Characterization and Diversity of Rhizobium Isolated From Root Nodules of Mid-Altitude Climbing Bean (Phaseolus vulgaris L.) Varieties

Genetic Characterization and Diversity of Rhizobium Isolated From Root Nodules of Mid-Altitude Climbing Bean (Phaseolus vulgaris L.) Varieties

Probiotic activities of Rhizobium laguerreae on growth and quality of spinach

Characterization of Indigenous Rhizobia Strains Associated to Soybean [Glycine max (L.) Merrill] in Benin

Contact Info

Product

Resources

About