Isolation and Characterization of Multidrug-Resistant Escherichia coli and Salmonella spp. from Healthy and Diseased Turkeys

Abstract

Diseases caused by Escherichia coli (E. coli) and Salmonella spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) E. coli and Salmonella spp. in healthy and diseased turkeys. A total of 30 fecal samples from healthy turkeys and 25 intestinal samples from diseased turkeys that died of enteritis were collected. Bacterial isolation and identification were based on biochemical properties and polymerase chain reaction (PCR). Antibiogram profiles were determined by disk diffusion. The tetracycline-resistance gene tetA was detected by PCR. All samples were positive for E. coli. Only 11 samples (11/30; 36.67%) were positive for Salmonella spp. from healthy turkeys, whereas 16 (16/25; 64%) samples were positive for Salmonella spp. from diseased turkeys. E. coli isolated from diseased turkeys showed higher resistance to levofloxacin, gentamicin, chloramphenicol, ciprofloxacin, streptomycin, and tetracycline. Salmonella spp. isolated from healthy turkeys exhibited higher resistance to gentamicin, chloramphenicol, ciprofloxacin, streptomycin, imipenem, and meropenem. All E. coli and Salmonella spp. from both healthy and diseased turkeys were resistant to erythromycin. Salmonella spp. from both healthy and diseased turkeys were resistant to tetracycline. Multidrug resistance was observed in both E. coli and Salmonella spp. from diseased turkeys. Finally, the tetA gene was detected in 93.1% of the E. coli isolates and in 92.59% of the Salmonella spp. isolates. To the best of our knowledge, this is the first study to isolate and characterize tetA-gene-containing MDR E. coli and Salmonella spp. from healthy and diseased turkeys in Bangladesh. Both microorganisms are of zoonotic significance and represent a significant public health challenge.

Virulence Determinants and Multidrug Resistance of Escherichia coli Isolated from Migratory Birds

“…The final confirmation of E. coli was done by polymerase chain reaction (PCR) assays targeting the malB gene (Table 5). For PCR, the genomic DNA was extracted from pure cultures of E. coli by the boiling method as previously described [61,62]. In brief, a pure colony from freshly grown culture was added into 100 µL phosphate buffer solution (PBS) in a sterile Eppendorf tube and mixed by gentle vortexing.…”

Folic Acid Absorption Characteristics and Effect on Cecal Microbiota of Laying Hens

“…These discrepancies might be attributed to differences in intestinal microbiota between humans and chickens. Additionally, Escherichia coli and Salmonella are major pathogen in poultry industry (46)(47)(48), the biochemical modulation of folate may potentially help the host cope with those challenging situations (49) and increase the host survival (50). Nevertheless, they were not detected by 16S sequencing in the present study, and a further study is required.…”

Isolation of multidrug-resistant Escherichia coli from turkeys in Dinajpur, Bangladesh, and their antibiogram profile

“…The isolates were 100% resistant to amoxicillin, azithromycin, erythromycin, tetracycline, bacitracin, cephalexin, nalidixic acid, vancomycin, methicilin, piperacillin, pefloxacin, novobiocin, cefepime, trimethoprim, netilmicin, and aztreonam. Several antibiotics were also used by Tawyabur et al [ 35 ] and reported similar results. Moreover, Tawyabur et al [ 35 ] studied the prevalence of multidrug-resistant E. coli in diseased and healthy turkeys.…”

“…Several antibiotics were also used by Tawyabur et al [ 35 ] and reported similar results. Moreover, Tawyabur et al [ 35 ] studied the prevalence of multidrug-resistant E. coli in diseased and healthy turkeys. However, they did not find any significant difference in the antibiotics resistance pattern between these two groups.…”