Isolation of multidrug-resistant Escherichia coli from turkeys in Dinajpur, Bangladesh, and their antibiogram profile

Kundu, Tonmoy; Rumi, Nazmi Ara; Hossain, Khaled; Rahman, Shajedur; Hossain, Mirdha Md Kamal; Halder, Joyanta

doi:10.5455/javar.2021.h486

Cited by 3 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The supernatants were incubated at 37°C for 30 minutes with 5l RNase A (5mg/ml in 0.5 M NaCl, 0.01 M EDTA). PCR protocol was followed by the previous [17]. Applied Biosystems Genetic Analyzer 3130 was used to sequence the genome of A. xylosoxidans at the National Institute of Biotechnology, Savar.…”

Section: Dna Sequencing and Phylogenic Tree Analysismentioning

confidence: 99%

Molecular Characterization and Antibiogram Study of Bacteria Isolated from Dental Plaque Samples from Dental Carries Patients in Northern Bangladesh

Rumi¹,

Hosen²,

Islam³

et al. 2023

Act Scie Dental

View full text Add to dashboard Cite

The attachment of salivary bacteria initiates the formation of dental plaque to the acquired pellicle covering the tooth surface. This Research aimed to investigate the prevalence and antibiogram study of isolated bacteria from Tasmia dental care Saidpur, Bangladesh. In this study, we selected 200 patients (aged 20-60 years) to isolate desired isolates. 16S rRNA gene sequencing techniques were applied for molecular confirmation of isolated bacteria. 55.83% of patients exhibited molar caries, 27.5% between molar and premolar, 12.5% at the incisor, and 4.17% at the cervical border. Dental caries was (P < 0.001) prevalent in age groups 31-40 (82.86%), 41-50 (72.22%), and above 50 (34.29%), respectively. In contrast to patients from high-and middle-income households, those from low-income families had a considerably higher prevalence of dental caries (79.78%) (P < 0.001). Dental caries was observed to be substantially (P < 0.001) more prevalent among the illiterate (84.0%) than among the educated (25.0%) or the very worried (8.33%). Staphylococcus spp. (33.33%), E. coli (25.0%), Streptococcus spp. (20.0%), Acromobacter xylosoxidans (16.67%) and Pseudomonas spp. (5.0%) were the most often isolated bacteria. Acromobacter_ xylosoxidans_strain_LMG_1863 was identified with 144bp in this Research. A. xylosoxidans, and E. coli were resistant to over six antibiotics. At the same time, all Pseudomonas spp.were resistant except for Ciprofloxacin and colistin. Staphylococcus spp. and Streptococcus spp. were also resistant to more than five antibiotic discs. Triclosan and fluoride-containing antibiotic-free toothpaste can be the best preventive methods for cavities.

show abstract

Section: Dna Sequencing and Phylogenic Tree Analysismentioning

confidence: 99%

Molecular Characterization and Antibiogram Study of Bacteria Isolated from Dental Plaque Samples from Dental Carries Patients in Northern Bangladesh

Rumi¹,

Hosen²,

Islam³

et al. 2023

Act Scie Dental

View full text Add to dashboard Cite

show abstract

Detection of Zoonotic Potential of Salmonella and Escherichia coli Isolated from Ostriches and Determine Their Antibiogram Study

2023

AJPAB

View full text Add to dashboard Cite

The present research was conducted for molecular characterization of important zoonotic bacteria isolated from different samples in ostrich and also determined their antimicrobial activity. For this current research, 32 samples were randomly collected from 8 ostriches at different ages, of which 8 were oropharyngeal, 8 were cloacal swabs, 8 were environmental sand samples, and 8 were feces samples. In addition, the bacteria were isolated and identified by using standard microbiological methods, including cultural, biochemical and molecular techniques. 16S rRNA gene was used to detect Escherichia coli and Salmonella spp. molecularly. The Kirby-Bauer disc diffusion method was used to determine the antibiotic sensitivity test. Out of 32 samples, E. coli 8 (53.33%) and Salmonella spp. 7 (46.67%) were identified in young ostrich, while in adult ostrich, E. coli 2 (40%) and Salmonella spp. 3 (60%) were detected. According to our study, E. coli was the most predominant isolate found in cloacal swabs and ostrich feces. Escherichia coli were most sensitive to Amoxicillin and Azithromycin (100%), followed by Kanamycin, Chloramphenicol and Gentamicin (75%), while 100% resistant to Piperacillin, Bacitracin, Tetracycline, Cloxacillin, Novobiocin, Cefixime. Salmonella spp. was 100% sensitive to Azithromycin and also 100% resistant to Tetracycline, Piperacillin, Bacitracin, Chloramphenicol and Methicillin. Our research concluded that E. coli and Salmonella spp. are multi-drug resistant bacteria, and appropriate antibiotics should be used in ostrich farms to protect the multi-drug resistant bacteria. We suggest farm owners increase public awareness about zoonotic diseases and those working on ostrich farms.

show abstract

Isolation, identification and antibiogram of verotoxin producing Escherichia coli from raw salad vegetables at Jashore, Bangladesh

Meghla¹,

Mridha²,

Rana³

et al. 2021

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Verotoxin-producing E. coli are getting an emergence for human health and day by day, it is also becoming more alarming in the fact that, they are acquiring multidrug-resistant profiles. This study aimed at isolation and identification of verotoxigenic E. coli followed by the antibiogram of the species from salad vegetables at Jashore district in Bangladesh. A total of 119 raw vegetable salad samples were collected from different areas of the Jashore district. The collected samples were initially enriched in nutrient broth and then used for streak plating on MacConkey and followed by Eosin Methylene Blue (EMB) agar media. Escherichia coli species were isolated and identified following observation of the cultural, microscopic, and biochemical characteristics and further analyzed to determine the presence of verotoxin (VT) producing genes through polymerase chain reaction (PCR) using specific primers (VT1, VT2, and Eae). A panel of antibiotics were tested following the disc diffusion method for determining the antibiotic profile. Total 55 (46.22%) E. coli was isolated phenotypically. 6 (10.9%) isolated E. coli showed positive by amplifying verotoxin-producing gene vt1, but none of the samples were positive vt2 and eaeA gene. Antibiogram for 55 E. coli isolates showed the highest resistance to Erythromycin (83.64%), Streptomycin (78.18%), Ceftriaxone (70.91%). On the other hand, the most heightened susceptibility was observed for Nalidixic acid (76.36%). Moreover, six vt1 positive isolates showed a multidrug-resistant profile. Thus, the presence of toxigenic genes in the isolates suggests the proper maintenance and regular monitoring of raw salad items should be increase to ensure healthy life in this region.

show abstract

Isolation of multidrug-resistant Escherichia coli from turkeys in Dinajpur, Bangladesh, and their antibiogram profile

Cited by 3 publications

References 20 publications

Molecular Characterization and Antibiogram Study of Bacteria Isolated from Dental Plaque Samples from Dental Carries Patients in Northern Bangladesh

Molecular Characterization and Antibiogram Study of Bacteria Isolated from Dental Plaque Samples from Dental Carries Patients in Northern Bangladesh

Detection of Zoonotic Potential of Salmonella and Escherichia coli Isolated from Ostriches and Determine Their Antibiogram Study

Isolation, identification and antibiogram of verotoxin producing Escherichia coli from raw salad vegetables at Jashore, Bangladesh

Contact Info

Product

Resources

About