Isolation and characterization of Newcastle disease virus from vaccinated commercial layer chicken

Balachandran, P.; Srinivasan, Prasanna; Sivaseelan, S.; Balasubramaniam, G.A.; Murthy, T.R. Gopala Krishna

doi:10.14202/vetworld.2014.457-462

Cited by 14 publications

(16 citation statements)

References 14 publications

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“…The present report shows the vaccination program applied could not protect the birds from clinical signs; however it protected from full 100% mortality characterized in fully susceptible flocks. Similar incidences have been reported by Musa et al (2010) and Balachandran et al (2014). The lentogenic B1 and Lasota vaccine strains of low virulence are commonly used worldwide, and can provide protection against virulent NDV if the vaccines are viable, administered correctly to healthy birds and time is allowed for an appropriate immune response to develop prior to exposure to the challenge virus (Cornax et al, 2012: Kapczynski andKing, 2005).…”

Section: Discussionmentioning

confidence: 62%

Outbreak investigation of Newcastle disease virus from vaccinated chickens in Eritrea

Mihreteab¹,

Gide²,

Nguse³

et al. 2017

Afr. J. Biotechnol.

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Newcastle disease (ND) is one of the most important infectious viral diseases of poultry in Eritrea.Diagnosis of ND virus infection in Eritrea has been mainly based on clinical examination and post mortem lesions. This study describes ND diagnosis using reverse transcription polymerase chain reaction PCR (RT-PCR), virus isolation and serological techniques (haemaglutination inhibition test) from two times vaccinated 40 days old chicks in Keren poultry farm. Generally clinical signs and postmortem lesions were characterized by rapid onset, death without visible lesions, ruffled plumage, dyspnea, in appetite, weakness and green colored diarrhea. Clinical samples collected were cloacal swabs. Observed postmortem lesions were tracheal hemorrhage, hemorrhage in proventriculus especially at the junction between esophagus and proventriculus and haemorrhage in caecal tonsils. Samples collected after postmortem include spleen, intestine and caecal tonsils. High titer of specific ND virus (NDV) antibody was detected indicating the occurrence of recent infection. ND virus was isolated from aseptically collected samples using embryonated chicken eggs. RT-PCR assay described was able to detect ND virus from cloacal swabs, spleen, intestine and caecal tonsils. Therefore, the described RT-PCR assay can be adopted for rapid and confirmative detection of NDV in Eritrea. Additionally, prevention of ND should not depend only on vaccination.

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Section: Discussionmentioning

confidence: 62%

Outbreak investigation of Newcastle disease virus from vaccinated chickens in Eritrea

Mihreteab¹,

Gide²,

Nguse³

et al. 2017

Afr. J. Biotechnol.

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“…The velogenic of NDV caused hemorrhage in all body systems. Hemorrhage was especially in the occipital part [ 7 ]. The infective dose of the ECEs was 2 3 , whereas the infective dose in the chicken was 2 6 [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Embryonic death was observed daily for 7 days and evaluated through candling and recorded embryonic mortality. The highest dilution in which all embryos die was calculated as mean lethal dose [ 7 ]. Viral virulence was categorized by three classifications: Embryo mortality <60 h (Velogenic), 60-90 h (Mesogenic), and > 90 h (Lentogenic) [ 3 ].…”

Section: Methodsmentioning

confidence: 99%

“…NDV virulence detection is important for vaccination and eradication programs [ 7 ]. This study aimed to evaluate the effect of administering various doses of NDV infection to pathogenicity cause death and organ disorders in ECEs and as well as protein profiles in allantoic fluid (AF) as the basic research reference for the development of biomolecular research.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Qosimah¹,

Murwani²,

Sudjarwo³

et al. 2018

Vet World

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Background and Aim:Newcastle disease virus (NDV) is an obligate intracellular parasite. Virus can only live on living cells. The embryonated chicken eggs (ECEs) are one of the growth media of virus that is a cheap, easy to do, and accurate for showing patterns of virus change in the host. Higher virus titers indicate the higher number of viruses and more virulent to infect host. This research aimed to investigate the effect of different level of NDV titer infection in ECEs on protein profile, embryonic length, mortality, and pathological change.Materials and Methods:The study used a completely randomized design of six treatments and seven replications. The treatments were different level of NDV titer infection in allantoic fluid (AF) of 9-11 days ECEs, i.e., P1=20, P2=26, P3=27, P4=28, P5=29, and P6=210 hemagglutination unit (HAU). All samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Data were analyzed using one-way ANOVA with p=0.05 for length of the embryo and descriptive analysis for embryo mortality, pathology change, and protein band.Results:The result showed that protein profile of NDV-infected ECEs of all different levels is more complex than protein profile of no NDV-infected ECEs. NDV infected of all different levels showed longer size embryo, higher mortality embryo at the first 2 days, and higher occurrence of hemorrhagic in all part of bodies of embryo than those of no NDV infected.Conclusion:It was concluded that NDV infection of all different level decreased health conditions of chicken embryo of ECEs of 9-11 days old. Different level of NDV infection of ECEs of 9-11 days old showed no significantly different embryo profiles. However, all of the NDV-infected embryos were shorter, death on the 2nd day, and suffered more hemorrhage on all body surfaces than uninfected NDV embryos.

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“…Diagnosis steps that have been established by pathologist in the field in determining the course of the disease are still relying on ND pathognomonic symptoms and reported lesions. Lesions most reported in ND infection is matting of vent feathers, petechiae hemorrhage on the tip of proventriculus papilla, ventriculus, intestine and cecal tonsil, congestion of trachea and lung, and degeneration of ovarian follicles [ 4 , 8 - 10 ].…”

Section: Introductionmentioning

confidence: 99%

Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia

Etriwati¹,

Ratih²,

Handharyani³

et al. 2017

Vet World

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Aim:The aim of the study was to examine pathology and the distribution pattern of Newcastle disease virus (NDV) in internal organs of chickens from a field case using immunohistochemical staining.Materials and Methods:10 groups of broiler, layer, and domestic chicken were collected from necropsy room Division of Pathology, Bogor Agricultural University. These chickens were originated from West Java and collected based on pathologist diagnosis as suspect of Newcastle disease (ND). They were subsequently confirmed positive of ND with real-time-reverse transcription polymerase chain reaction assay. The respiratory, circulatory, digestive, lymphoreticular and central nervous systems were collected for histopathology examination.Results:The gross pathology and histopathology changes were tracheitis, pneumonia, pericarditis, myocarditis, catarrhal proventriculitis, catarrhal enteritis, typhlitis, perihepatitis, pancreatitis, nephritis interstitial, splenitis, atrophy of Bursa Fabricius, and encephalitis.Conclusion:The distribution pattern of NDV in internal organs of chickens from a field case in this study is similar with a previous reported pattern in systemic cases of the internal chicken organs. High intensity of immunohistochemistry stain result was detected in trachea, lung, proventriculus, duodenum, cecal tonsil, kidney, and brain.

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Isolation and characterization of Newcastle disease virus from vaccinated commercial layer chicken

Cited by 14 publications

References 14 publications

Outbreak investigation of Newcastle disease virus from vaccinated chickens in Eritrea

Outbreak investigation of Newcastle disease virus from vaccinated chickens in Eritrea

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia

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