Isolation and Characterization of NP‐POL Nonapeptide for Possible Therapeutic Use in Parkinson’s Disease

Abstract

Colostrum and milk are the initial mammalian nourishment and rich reservoir of essential nutrients for newborn development. Bioactive peptides isolated from natural sources, such as colostrum, serve as endogenous regulators and can be used as alternative therapeutic agents in the treatment of neurodegenerative diseases. One example is the previously unknown NP-POL nonapeptide isolated from Colostrinin. In the present study, we investigated a method of NP-POL nonapeptide isolation using Bio-Gel P2 molecular sieve chromatography. We showed the protective effect of NP-POL on 6-hydroxydopamine- (6-OHDA-) induced neurotoxicity using rat adrenal pheochromocytoma (PC12 Tet On) cells. Treatment of PC12 cells with NP-POL nonapeptide reduced 6-OHDA-induced apoptosis and caused transient phosphorylation of extracellular signal-regulated kinases (ERK 1/2), which were shown to promote cell survival. NP-POL nonapeptide also protected neuronal cells against oxidative injury induced by 6-OHDA. These results showed a potential use of NP-POL in the therapy of Parkinson's disease.

Neuroprotective effects of salidroside against 6-OHDA-induced oxidative stress in PC12 cells

“…Previous studies have demonstrated that 6-OHDA induce neurotoxicity in PC12 cells to construct a cellular model of PD. [22][23][24][25] Firstly, the cytotoxic effect of 6-OHDA on PC12 cells were measured via a CCK8 assay. PC12 cells were treated with different doses of 6-OHDA (50, 100, 150, and 200 mM) for 24 h; at concentrations of over 50 mM the viability of PC12 cells was significantly affected, and a concentration of 100 mM or higher did not decrease the cell viability (Fig.…”

The Reasons Behind the Salubrious of Colostrum on the Cognitive Functions: A Systematic Review

“…43 Another study demonstrated that the active ingredient in colostrinin reduced rather than induced the neurotoxicity of adrenal pheochromocytoma cells that was induced by 6-hydroxydopamine, via transient activation of the ERK pathway. 44 Lactoferrin protects the neuronal cell by stabilizing the hypoxiainducible factor-1α via its up-regulatory effects on the prolyl hydroxylase enzyme and thereby prevents the formation of the abnormal isoform prion protein. 45 Other researchers demonstrated that insulin growth factor-1, which is available in the BC, plays an important role in the suppression of neurodegeneration via its effect on the generation of reactive oxygen species and improves mitochondrial function by blocking the effect of the cellular prion protein upon the mitochondria.…”