1983
DOI: 10.3136/nskkk1962.30.339
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Isolation and characterization of oligosaccharides produced from sucrose by transglucosylation action of Serratia plymuthica.

Abstract: The oligosaccharides produced by the title reaction were dealt with. After removal of the most of palatinose the reaction mixture was used as the sample. Carbon-celite chromatography of the sample(40g, 66•‹ Brix)gave three saccharides(saccharide A, B and C), besides palatinose and sucrose. Saccharide A was obtained as syrup(2.0g)and identified as 1-ƒ¿-D-glucopyranosyl-Dfructofuranose by chemical reactions and some chromatographic analyses such as HPLC, GLC, TLC and PC, and by the fragmentation analysis of GC-M… Show more

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Cited by 21 publications
(14 citation statements)
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“…Several microorganisms, such as Protaminobacter rubrum (32), Serratia plymuthica (11), Klebsiella planticola (18), and Erwinia rhapontici (5), have been found to form palatinose and trehalulose from sucrose. The adaptive role of sucrose isomer formation is unclear.…”
mentioning
confidence: 99%
“…Several microorganisms, such as Protaminobacter rubrum (32), Serratia plymuthica (11), Klebsiella planticola (18), and Erwinia rhapontici (5), have been found to form palatinose and trehalulose from sucrose. The adaptive role of sucrose isomer formation is unclear.…”
mentioning
confidence: 99%
“…Isomaltulose synthase has been studied by many investigators from the point of intramolecular transglycosylation. Protaminobacter rubrum, 9,1 0) Serratia plymutica, [10][11][12] Erwinia, 13,14) and Klebsiella 15 ) are known to produce this enzyme. Isomaltulose synthase catalyzes the conversion of sucrose into the structural isomers, isomaltulose (paratinose, 6-0-I1-o-glucopyranosyl-o-fructofuranose) and trehalulose (l-O-I1-o-glucopyranosyl-o-fructofuranose) simultaneously.…”
Section: Discussionmentioning
confidence: 99%
“…A isomaltulose é também utilizada na produção de isomalte, uma mistura de açúcar álcool, de baixo valor calórico e baixo potencial cariogênico. No estudo da influência dos componentes do meio de cultivo, na produção de glicosiltransferase, em frascos agitados, foi obtido maior atividade da enzima (12,8 unidades de atividade/mL do meio de cultura) em meio de cultura A constituído de melaço 12% (p/v) de sólidos solúveis totais, peptona 4% (p/v) e extrato de carne 0,4% (p/v). No estudo do efeito do tempo e da temperatura na fermentação da linhagem de Erwinia sp D12, em fermentador New Brunswick de 3L, contendo meio de cultura A, foi obtida maior atividade de glicosiltransferase (15,6 unidades de atividade/ mL de meio de cultura) na fase exponencial de crescimento após 8 horas de fermentação a 30ºC.…”
Section: Introductionunclassified