Isolation and characterization of ovine follicle stimulating hormone

Cahill, C.L.; Shetlar, M. R.; Payne, R. W.; Endecott, Boyd R.; Li, Yu-Teh

doi:10.1016/0005-2795(68)90256-0

Cited by 23 publications

(3 citation statements)

References 20 publications

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“…Electrophoretically, follitropin was more acidic than lutropin and thyrotropin, and migrated further towards the anode (Plate 1). This acidic nature of bovine follitropin is probably due to the presence of sialic acid in the molecule; presence of sialic acid has been reported in purified follitropin preparations ofsheep (Papkoffet al, 1967a;Cahill et al, 1968) and human (Papkoff et al, 1967b;Reichert et al, 1968).…”

Section: Discussionmentioning

confidence: 83%

Purification and properties of bovine pituitary follitropin

Cheng¹

1976

Biochemical Journal

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A reproducible procedure was developed for the purification of follitropin from frozen bovine pituitary glands. The method involved precipitation with (NH4)2SO4 and acetone, followed by ion-exchange column chromatography on CM-cellulose and DEAE-cellulose and gel filtration on Sephadex G-100. A specific radioligand-receptor assay for follitropin was used to locate the activity in eluates after column chromatography and gel filtration. The potency of the highly purified bovine follitropin as measured by Steelman-Pohley bioassay was 164 times that of NIH-FSH-S1 standard preparation. They yield of bovine follitropin was 2.9 mg/kg of frozen pituitary glands. Electrophoretically, bovine follitropin was more acidic in nature and migrated further towards the anode than lutropin and thyrotropin. The elution volume of bovine follitropin by gel filtration on Sephadex G-100 was very similar to that of bovine lutropin. The amino acid composition of bovine follitropin was similar to that of sheep and human follitropin, being rich in lysine, aspartic acid, threonine, serine, glutamic acid and half-cystine.

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Section: Discussionmentioning

confidence: 83%

Purification and properties of bovine pituitary follitropin

Cheng¹

1976

Biochemical Journal

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“…The primary reason for this is most probably the difference in the methodology. Direct colorimetric analysis of an unhydrolyzed glycoprotein often gives high results (Cahill et al, 1968). The a subunit contains a slightly higher amount of total hexoses and fucose than does the /3.…”

Section: Resultsmentioning

confidence: 99%

Equine luteinizing hormone and its subunits. Isolation and physicochemical properties

Landefeld¹,

McShan²

1974

Biochemistry

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“…Recently, however, FSH has been obtained in a high state of purity (Papkoff el al. 1967;Hashimoto et al 1966;Jutisz et al 1965;Cahill et al 1968), yet the ICSH contamination as determined by the ascorbic acid depletion test, is still relatively high, Papkoff (1967) (1968) the ICSH activity cannot be detected.…”

Section: Discussionmentioning

confidence: 99%

Steroidogenic Properties of Gonadotrophins

Gospodarowicz¹

1969

Acta Endocrinologica

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The effect in vitro of the interstitial cell stimulating hormone (ICSH or LH), of pregnant mare serum gonadotrophin (PMSG) and of lactogenic hormone (LTH), on the synthesis of progesterone in slices of ovaries taken from pseudopregnant rats was investigated. It was found that ovine ICSH stimulated the synthesis of progesterone at a level of 0.01 μg/g of tissue; PMSG had no effect at a level of 0.1 μg/g of tissue, and a stimulating effect at higher levels; LTH had no steroidogenic effect. In addition, the effects of two follicle-stimulating hormone (FSH) preparations, one largely free of ICSH and the other containing minute quantities of ICSH, were studied in hypophysectomized rats and on the stimulation of progesterone synthesis in the ovaries of pseudopregnant rat. At concentration of less than 25 μg, ovine FSH had no effect on uterine growth and on interstitial tissue repair in the hypophysectomized female rat, and also had no effect on the synthesis of progesterone at concentrations of less than 50 μg/g of tissue. The ICSH contaminant in FSH was determined by comparing the steroidogenic properties of FSH with those of ICSH. Furthermore, studies with combinations of FSH and ICSH demonstrated that quantities of ICSH which in themselves are unable to stimulate steroidogenesis, are effective when combined with FSH.

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Isolation and characterization of ovine follicle stimulating hormone

Cited by 23 publications

References 20 publications

Purification and properties of bovine pituitary follitropin

Purification and properties of bovine pituitary follitropin

Equine luteinizing hormone and its subunits. Isolation and physicochemical properties

Steroidogenic Properties of Gonadotrophins

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