Isolation and characterization of phytoferritin from pea (<i>Pisum sativum</i>) and Lentil (<i>Lens esculenta</i>)

Crichton, Robert R.; Ponce-Ortiz, Yezid; Koch, Michel H. J.; Parfait, R.; Stuhrmann, H. B.

doi:10.1042/bj1710349

Cited by 70 publications

(32 citation statements)

References 17 publications

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“…USA 79 (1982) 3119 as a donor of Cu2" to the corresponding apoenzymes. Plants contain phytoferritin, yeasts contain a ferritin-like protein (25). These are high molecular weight proteins.…”

Section: Resultsmentioning

confidence: 99%

Ferritin: a zinc detoxicant and a zinc ion donor.

Price¹,

Joshi²

1982

Proc. Natl. Acad. Sci. U.S.A.

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Rats were injected with 1 mg of Zn2' as zinc sulfate or 2 mg of Cd2+ as cadmium sulfate per kg of body weight on a daily basis. After seven injections, ferritin and metallothionein were isolated from the livers of the rats. Significant amounts of zinc were associated with ferritin. Incubation ofsuch ferritin with apoenzymes of calf intestinal alkaline phosphatase, yeast phosphoglucomutase, and yeast aldolase restored their enzymic activity. The amount of zinc injected was insufficient to stimulate significant synthesis ofmetallothionein, but similar experiments with injection of cadmium did stimulate the synthesis of metallothionein. The amount of Zn2`in ferritin of Cd-injected rats was greater than that in ferritin in Zn-injected rats, which was greater than that in ferritin of normal rats. Thus at comparable protein concentration ferritin from Cd-injected rats was a better. Zn2+ donor than was ferritin from Zn-injected or normal animals. Ferritin is a normal constituent of several tissues, whereas metallothionein is synthesized under metabolic stress. Thus ferritin may function as a "metal storage and transferring agent" for iron and for zinc. It is su gested that ferritin probably serves as the initial chelator for Zn + and perhaps other metal ions as well and that under very high toxic levels of metal ions the synthesis of metallothionein is initiated as the second line of defense.

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“…USA 79 (1982) 3119 as a donor of Cu2" to the corresponding apoenzymes. Plants contain phytoferritin, yeasts contain a ferritin-like protein (25). These are high molecular weight proteins.…”

Section: Resultsmentioning

confidence: 99%

Ferritin: a zinc detoxicant and a zinc ion donor.

Price¹,

Joshi²

1982

Proc. Natl. Acad. Sci. U.S.A.

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“…Less than 0.2 % of the iron determined in roots, when young fruits start to develop, could be stored inside ferritin, even ifthe ferritin were completely saturated with iron (i.e. about 5000 iron atoms per molecule ;Crichton et al, 1978). We have no idea where this root iron is located.…”

Section: Seed Ferritin Is Processed and Disappears During Germinationmentioning

confidence: 97%

Ferritin accumulation and degradation in different organs of pea (Pisum sativum) during development

Lobréaux

Briat

1991

Biochemical Journal

150

119

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Iron concentration and ferritin distribution have been determined in different organs of pea (Pisum sativum) during development under conditions of continuous iron supply from hydroponic cultures. No ferritin was detected in total protein extracts from roots or leaves. However, a transient iron accumulation in the roots, which corresponds to an increase in iron uptake, was observed when young fruits started to develop. Ferritin was detectable in total protein extracts of flowers and pods, and it accumulated in seeds. In seeds, the same relative amount of ferritin was detected in cotyledons and in the embryo axis. In cotyledons, ferritin and iron concentration decrease progressively during the first week of germination. Ferritin in the embryo axis was processed, and disappeared, during germination, within the first 4 days of radicle and epicotyl growth. This degradation of ferritin in vivo was marked by a shortening of a 28 kDa subunit, giving 26.5 and 25 kDa polypeptides, reminiscent of the radical damage occurring in pea seed ferritin during iron exchange in vitro [Laulhere, Laboure & Briat (1989) J. Biol. Chem. 264, 3629-3635]. Developmental control of iron concentration and ferritin distribution in different organs of pea is discussed.

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“…The molecular mechanism of this regulation is not known. Plant ferritins have been identified from both dicotyledons (4,15,20) and monocotyledons (8). They appear to be coded by nuclear DNA and plastid localized (22).…”

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confidence: 99%

Iron Induction of Ferritin Synthesis in Soybean Cell Suspensions

Proudhon¹,

Briat²,

Lescure³

1989

Plant Physiol.

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In animal cells specialized for iron storage, iron-induced accumulation of ferritin is known to result from a shift of stored mRNA from the ribonucleoprotein fraction to polysomes. Previous reports with bean leaves suggested that in plants iron induction of ferritin synthesis would result from a regulation at the transcriptional level (F van der Mark, F Bienfait, H van der Ende cultures, the mechanism of regulation of ferritin synthesis in response to iron does not result from recruitment of preexisfing mRNA. They confirm that in plant systems, ferritin synthesis results from increased transcription of the corresponding genes.

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Isolation and characterization of phytoferritin from pea (Pisum sativum) and Lentil (Lens esculenta)

Cited by 70 publications

References 17 publications

Ferritin: a zinc detoxicant and a zinc ion donor.

Ferritin: a zinc detoxicant and a zinc ion donor.

Ferritin accumulation and degradation in different organs of pea (Pisum sativum) during development

Iron Induction of Ferritin Synthesis in Soybean Cell Suspensions

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