Isolation and characterization of pigmentation mutants of <i>Micrococcus roseus</i>

Schwartzel, Edmund H.; Cooney, J. J.

doi:10.1139/m74-156

Cited by 13 publications

(13 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In M. roseus, however, carotenoids do not appear to protect the cells from photodynamic killing (29). In fact, cells of M. roseus in which carotenoid biosynthesis was stopped with diphenylamine showed very small amounts of carotenoids but exhibited greater resistance to photodynamic killing than did the pigmented wild-type M. roseus.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure, and interaction with synthetic membranes

1991

View full text Add to dashboard Cite

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain was purified to homogeneity from methanol extracts of dried cells by reverse-phase liquid chromatography and was designated P-3. On the basis of the UV-visible, infrared, mass, and 'H nuclear magnetic resonance spectra of P-3, it was identified as bisdehydro-,-carotene-2-carboxylic acid. The pigment interacted with synthetic membranes of phosphatidylcholine and dimyristoyl phosphatidylcholine and stabilized the membranes. These results also indicate that P-3 is different from canthaxanthin, the major carotenoid pigment from a mesophilic M. roseus strain.Carotenoid pigments are present in a wide variety of bacteria, algae, fungi, and plants (1,23 (16,17). Cells were pelleted by centrifugation at 1,000 x g for 10 min, washed free of medium with distilled water, and freeze-dried to a powder. Methanol (100 ml) was added to about 1 g of freeze-dried cells, and the mixture was vortexed until the methanol layer turned red. The entire suspension of cells was centrifuged (5,000 x g for 5 min), and the methanol layer containing the crude pigment was recovered. The pale pink 7911 JOURNAL

show abstract

Section: Discussionmentioning

confidence: 97%

“…For M. luteus, it was found to be a dihydroxy C50 carotenoid (35)(36)(37), and for M. roseus the pigments were found to be mainly a or 1B carotene derivatives, with canthaxanthin as the main pigment (3,28,38). Studies have indicated that carotenoids in M. roseus do not protect the bacterium against photodynamic killing (29).…”

mentioning

confidence: 99%

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure, and interaction with synthetic membranes

1991

View full text Add to dashboard Cite

show abstract

“…Radiation effects observed in Micrococcus using toluidine blue as an exogenous photosensitizer are not comparable with the results presented here [25]. In Myxococcus xanthus carotenoid functions are related with an unusual accumulation of porphyrin [5], and in Micrococcus roseus ATCC 516 [6], at present classified as Kocuria rosea [19], photoprotection is provided by canthaxanthin, echinenone, 4-hydroxyechinenone, and other pigments with a β,β-carotene structure [26], which are structurally different from those found in RMB40. Sarcina lutea ATCC 9341 [3,4] was classified as Micrococcus luteus in 1977, but it was re-classified as Kocuria rhizophila in 2003 [27] and its pigments have not been characterized.…”

Section: Protective Effect Of Carotenoidsmentioning

confidence: 65%

The relationship between carotenoids and sunlight response in members of the family Micrococcaceae

Pezzoni

Costa

Pizarro

et al. 2011

J. Basic Microbiol.

View full text Add to dashboard Cite

The aim of this study was to compare the photoprotective effect of carotenoids in phylogentically related bacteria, which synthesize structurally different pigments. Two organisms were isolated from the same environment. Their 16S rDNA sequences and phenotypic characteristics identified them as members of the family Micrococcaceae. Reverse phase HPLC and absorption spectroscopy revealed that one of them, designated RMB40, synthesized 3 carotenoids with 9 conjugated double bonds, whilst the other, designated RMB42, synthesized a single and more hydrophobic pigment carrying 11 conjugated double bonds. Survival curves were obtained during sunlight exposure for both organisms and for carotenoid deficient mutants derived from them. Increased sunlight sensitivity was found in the carotenoidless mutant derived from RMB42. In contrast, pigment depletion had no appreciable effect on the sunlight response of RMB40. It is concluded that the structure of bacterial carotenoid probably exert an important influence on the effectiveness of these compounds to provide photoprotection in vivo.

show abstract

“…However, responsible enzymes for the formation of astaxanthin metabolites and their biological functions have not been elucidated. Canthaxanthinis another keto-carotenoid pigment found in green algae, bacteria, crustaceans and fish (Schwartzel & Cooney, 1972). Tyczkowski, Yagen, and Hamilton (1988) revealed that a portion of canthaxanthin was reduced to 4-hydroxyechinenone (4-hydroxy-4 0 -keto-beta, beta-carotene) that in turn was reduced in part to isozeaxanthin (4,4 0 -dihydroxy-beta,beta-carotene) was confirmed by high performance liquid chromatography analysis of tissues from chicks.…”

Section: Metabolism Of Oxygenated Carotenoidsmentioning

confidence: 88%

Metabolomics of carotenoids: The challenges and prospects – A review

Arathi

Sowmya

Vijay

et al. 2015

Trends in Food Science & Technology

View full text Add to dashboard Cite

Considerable progress in carotenoids research has been made to understand the carotenoid metabolism in animals including human. Epidemiological and clinical studies have correlated with dietary intake of carotenoids on reduction of vitamin A deficiency, age-related macular degeneration, cancer and cardiovascular diseases. Recent findings demonstrate the existence of carotenoid metabolites in vivo and their efficacy have made greater insight on prospecting carotenoid metabolites. Owing to their biological activity, exploration of analytical methods for the characterization of carotenoid metabolites is considered to be important before addressing the stability and bioactivity. Although few studies are available on carotenoid metabolites, their structural characterization in biological samples require a substantial refining of analytical protocols like isolation, purification, prerequisite of equipment parameters and robustness in hyphenated techniques.Recently, researchers have focused on biotransformation of carotenoids and made an attempt to screen their metabolites by high-throughput analytical strategies. However, till date there is no detailed analytical techniques available to fingerprint carotenoid metabolites, due to interference with complex biological matrices. This review highlights the carotenoid metabolism, possible bioconversion and available bio-analytical techniques to characterize metabolites in vivo. Further, advancement in sensitivity, mode of ionization and fragmentation patterns of metabolites were also discussed. The identification of carotenoid metabolites in system specific will have further insight in the emerging field of nutritional metabolomics.

show abstract

Isolation and characterization of pigmentation mutants of Micrococcus roseus

Cited by 13 publications

References 10 publications

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure, and interaction with synthetic membranes

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure, and interaction with synthetic membranes

The relationship between carotenoids and sunlight response in members of the family Micrococcaceae

Metabolomics of carotenoids: The challenges and prospects – A review

Contact Info

Product

Resources

About