Isolation and characterization of probiotic strains for improving oral health

Purification and primary characterization of a novel bacteriocin, LiN333, from Lactobacillus casei, an isolate from a Chinese fermented food

“…For bacteriocin production, a single colony of L. casei, was inoculated into TBB and incubated overnight 86 at 37 o C. A 5% aliquot of this overnight culture of was inoculated into TBB (1 L) and incubated for 48 h 87 at 37 o C. Indicator strains were streak by the radical streak method (Bosch et al, 2012) 4 o C), the sediment was recovered and resuspended in 5 mL of 10 mM formate buffer (pH 3.5). Dissolved 100 pellets were screened for antimicrobial activity using the well diffusion method (Wolf & Gibbons, 1996).…”

Various approaches for prevention of Dental caries with emphasis on Probiotics: A review

“…Lactic acid bacteria like Lactococcus lactis competitively inhibited S. mutans growth under nutritional deficiency, and the metabolites of S. mutans, including several exogenous molecular signals, enhanced the expression of genes related to nisin synthesis, nisA, nisB and nisI. Additionally, L. lactis effectively colonised the surface of tooth enamel, which showed substantially less decay with L. lactis adhesion compared to S. mutans adhesion (25,26).Twetman et al carried out a study to assess the effectivity of probiotics in caries reduction in children and reported a significant caries reduction in 3 to 4 year-old children after 7 months of daily consumption of probiotic milk (27).…”

Are we ready for caries prevention through bacteriotherapy?

“…Nonetheless, the proposed strains have not yet been tested in human clinical trials. 12,13 One selected candidate, however, has been evaluated in an animal model. Pathogen-free rats were inoculated with S. mutans and fed with a cariogenic diet supplemented with a heatkilled probiotic strain (L. paracasei DSMZ16671) or placebo.…”