Isolation and characterization of progenitor cells from surgically created early healing alveolar defects in humans: A preliminary study

Sant’Ana, Adriana Campos Passanezi; Damante, Carla Andreotti; Martinez, Maria Alejandra Frias; Valdivia, Maria Alejandra Medina; Karam, Paula Stefânia Hage; Oliveira, Flávia Amadeu de; Oliveira, Rodrigo Cardoso de; Gasparoto, Thaís Helena; Campanelli, Ana Paula; Zangrando, Mariana Schützer Ragghianti; Rezende, Maria Lúcia Rubo de; Greghi, Sebastião Luiz Aguiar; Passanezi, Euloir

doi:10.1002/jper.17-0665

Cited by 6 publications

(10 citation statements)

References 31 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that the tissue present in healing sockets at the third to fourth week is rich in woven bone and immature osteoblasts [37,[42][43][44][45]. SC-EHAD cell characteristics suggest its osteoprogenitor nature [31].…”

Section: Discussionmentioning

confidence: 99%

“…Fresh samples of SC-EHAD tissue exhibited CD34− and CD45−phenotypes, while SC-EHAD cells at later passages exhibited, besides that, a CD105−, CD166−, and collagen type I+ phenotype. These findings suggest that SC-EHAD is a possible source of progenitor cells [31]. However, the properties of this human cell lineage when cultivated on different implant surfaces are yet unknown.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human

et al. 2020

Self Cite

View full text Add to dashboard Cite

The aim of this study was to investigate the response of osteogenic cell lineage and gingival fibroblastic cells to different surface treatments of grade IV commercially pure Titanium (cpTi) disks. Material and methods: Grade IV cpTi disks with different surfaces were produced: machined (M), sandblasting (B), sandblasting and acid subtraction (NP), and hydrophilic treatment (ACQ). Surface microtopography characteristics and chemical composition were investigated by scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS). Adhesion and proliferation of SC-EHAD (human surgically-created early healing alveolar defects) and HGF-1 (human gingival fibroblasts) on Ti disks were investigated at 24 and 48 h, and osteogenic differentiation and mineralization were evaluated by assessing alkaline phosphatase (ALP) activity and alizarin red staining, respectively. Results: No significant differences were found among the various surface treatments for all surface roughness parameters, except for skewness of the assessed profile (Rsk) favoring M (p = 0.035 ANOVA). M disks showed a slightly higher (p > 0.05; Kruskal-Wallis/Dunn) adhesion of HGF-1 (89.43 ± 9.13%) than SC-EHAD cells (57.11 ± 17.72%). ACQ showed a significantly higher percentage of SC-EHAD (100%) than HGF-1 (69.67 ± 13.97%) cells adhered at 24 h. SC-EHAD cells expressed increased ALP activity in osteogenic medium at M (213%) and NP (235.04%) surfaces, but higher mineralization activity on ACQ (54.94 ± 4.80%) at 14 days. Conclusion: These findings suggest that surface treatment influences the chemical composition and the adhesion and differentiation of osteogenic cells in vitro. Clinical relevance: Hydrophilic surface treatment of grade IV cpTi disks influences osteogenic cell adhesion and differentiation, which might enhance osseointegration.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…A 66.29% ± 25.04% mean root coverage was achieved by the NFBG technique compared to 73.50% ± 25.53% achieved by SCTG, with no statistical differences between groups. The mean percentage of root coverage attained with NFBG is comparable to GTR‐based root coverage (16.7%‐100%), but results in significantly greater clinical attachment level gain and reduction in PD, as shown before for GTR‐based root coverage, especially in areas showing ≥ 5 mm of recession . This is particularly important in the treatment of deep recession defects where greater buccal attachment loss is observed.…”

Section: Discussionmentioning

confidence: 62%

“…Recently, our research group has isolated and characterized cells obtained from surgically created early healing alveolar defects (SC‐EHAD) in humans for the first time, based on the NFBG technique . Our findings suggested that SC‐EHAD is a possible source of progenitor cells, since cells obtained from healing bone granulation tissue were able to adhere to plastic, to express some markers of MSC, and to assume a spindle‐shaped morphology at earlier passages, showing alkaline phosphatase and mineralization activity.…”

Section: Discussionmentioning

confidence: 99%

“…This is particularly important at sites showing deep recession defects, in which soft tissue grafts show limited results. 26 Recently, our research group has isolated and characterized cells obtained from surgically created early healing alveolar defects (SC-EHAD) in humans for the first time, 27 based on the NFBG technique. 21 Our findings suggested that SC-EHAD is a possible source of progenitor cells, since cells obtained from healing bone granulation tissue were able to adhere to plastic, to express some mark- Comparing the results obtained in our study to others reported in the literature is difficult since this is the first investigation on NFBG-based root coverage.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Osteogenic cells transfer improving root coverage: A randomized clinical trial

Ferraz

Stuani

Passanezi

et al. 2019

J of Periodontal Research

Self Cite

View full text Add to dashboard Cite

Background and Objective The aim of this interventional, parallel‐assignment, single‐blinded, randomized, efficacy study was to investigate the efficacy of the treatment of gingival recessions by osteogenic cell transfer. This is the first randomized clinical trial of this nature. Material and Methods Treatment of Miller class I or II gingival recessions >4 mm was randomly defined by casual sorting and performed by newly forming bone graft (NFBG) and coronally advanced flap (CAF) or subepithelial connective tissue graft (SCTG) and CAF (control). Clinical examinations were performed by a single blinded examiner at baseline and at 3, 6, and 9 months after surgery. Parameters investigated were recession height (REC), probing depth (PD), clinical attachment level, gingival bleeding index (GBI), plaque index (PlI), and keratinized gingiva width (KGW). Results Both techniques achieved similar percentage of root coverage, but NFBG was more effective in pocket depth reduction, attachment level gain, and reduction of plaque accumulation and of bleeding on probing. Conclusions These findings suggest that the transfer of osteogenic cells by NFBG is able to improve clinical attachment level and to cover denuded roots. NFBG can be used as an alternative treatment of deep recessions (≥4 mm), where the restoration of lost periodontal tissues is intended.

show abstract

Research progress on application of dental stem cells in alveolar bone and periodontal regeneration

Liu,

Guo,

Guo

et al. 2024

MedComm – Future Medicine

View full text Add to dashboard Cite

Periodontal disease is a prevalent inflammatory condition affecting the tissues and bone surrounding the teeth, posing significant challenges to oral health. Current treatments often fall short due to incomplete removal of bacteria and plaque, irreversible damage to gums and bone, and their cost and time‐consuming nature. Stem cell therapy offers a promising alternative that potentially addresses these limitations. This review delves into the biology, mechanisms, and applications of dental stem cells (DSCs) in alveolar bone and periodontal regeneration. We begin with an overview of various stem cell types and their characteristics, highlighting their role in tissue regeneration and the mechanisms involved. Subsequently, we examine preclinical and clinical studies that demonstrate the efficacy and safety of DSC therapy, showcasing their potential in restoring periodontal health. The discussion also extends to the critical challenges and future directions in the field, emphasizing the necessity for standardized protocols and rigorous research to fully integrate DSC‐based therapies into routine clinical practice. This review underscores the transformative potential of DSCs in regenerative dentistry, aiming to provide a comprehensive understanding that can propel further advancements and ultimately improve patient outcomes in periodontal therapy.

show abstract

Isolation and characterization of progenitor cells from surgically created early healing alveolar defects in humans: A preliminary study

Abstract: Tissue removed from SC-EHAD is a possible source of progenitor cells.

Cited by 6 publications

References 31 publications

Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human

Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human

Osteogenic cells transfer improving root coverage: A randomized clinical trial

Research progress on application of dental stem cells in alveolar bone and periodontal regeneration

Contact Info

Product

Resources

About