Isolation and characterization of Pseudomonas sp. DX7 capable of degrading sulfadoxine

Abstract

Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp.based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l -1 sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30°C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l -1 and between 2.0 and 8.0 g l -1, respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxinecontaminated environments.

Purification and properties of a novel quizalofop-p-ethyl-hydrolyzing esterase involved in quizalofop-p-ethyl degradation by Pseudomonas sp. J-2

“…strain Jpyr-1 [26], and sulfadoxine by Pseudomonas sp. DX7 [27]. In this study, a pure bacterial strain identified to the genus Pseudomonas was isolated and efficiently degraded QPE.…”

Bacteria-mediated bisphenol A degradation

“…Among all these methods, biodegradation has been proved to be an advanced technique to remove various pollutants from the environment. In our lab, efforts were made to investigate the biotransformation of heavy metals, and biodegradation of sulfonamide drugs, nitrofurans drugs, and EDCs by bacterial strains and their potential application in bioremediation (Zhang et al 2012a(Zhang et al , 2012b(Zhang et al , 2012c(Zhang et al , 2013. Bacterial community is readily catabolized to BPA, and BPA-degrading bacterial strains were isolated and identified from various environments (Kang et al 2006a).…”

Degradation of furazolidone by bacteria Acinetobacter calcoaceticus T32, Pseudomonas putida SP1 and Proteus mirabilis V7

“…The cell pellets were washed twice with sterilized phosphate buffer (PBS), and adjusted to OD 600 of approximately 1. Zhang et al (2012). Briefly, bacterial cells were cultured in 10 ml MTM containing 5 mg l À1 FZD at 28 C for 2 days, and cells were pelleted by centrifugation at 10,000 Â g for 5 min.…”