Isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from calves and lambs with diarrhoea in India

Wani, S.A.; Bhat, Mashooq A.; Samanta, Indranil; Nishikawa, Yoshikazu; Buchh, A.S.

doi:10.1046/j.1472-765x.2003.01364.x

Cited by 65 publications

(80 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…in small ruminants from Turkey (Gokce et al 2010) and other states (Wani et al 2004), however, research on classification of enterovirulent E. coli and its virulence genes is limited. Recent studies have reported (Wani et al 2003;Bhat et al 2008;Bandyopadhyay et al 2011), the existence of at least one virulence gene in 41%, 36.7%, and 32.6% of E. coli isolates, respectively. In our study we found at least one virulence gene for EHEC or STEC, EPEC, ETEC, EIEC, and EAEC among 36.4% of the isolates.…”

Section: Resultsmentioning

confidence: 99%

Identification, characterization and molecular epidemiology of Escherichia coli isolated from lamb and goat kids with diarrhoea

Türkyılmaz

Eskiizmirliler²,

Tunalıgi̇l³

et al. 2013

Acta Vet. Brno

View full text Add to dashboard Cite

Neonatal diarrhoea is a serious health problem on commercial farms. Enterovirulent Escherichia coli is a significant aetiological agent of neonatal diarrhoea. In this work, identification and classification of E. coli isolates obtained from lambs and goat kids with diarrhoea were studied along with antibiotic resistance and clonal relationships of enterovirulent strains. A total of 107 E. coli strains isolated from animals on 43 farms were investigated. Specific virulence genes were determined by multiplex and uniplex polymerase chain reaction. Testing of antibiotic susceptibility was carried out by the Vitek II compact system. The relationship of E. coli isolates was determined by enterobacterial repetitive intergenic consensus polymerase chain reaction. A total of 39 (36.4%) enterovirulent E. coli strains were identified and of this 19 (48.7%) were shiga toxigenic, 12 (30.8%) enterotoxigenic and 8 (20.5%) enteropathogenic. Three isolates (7.7%) were found to be positive for extended spectrum beta lactamase; 10 (25.6%) isolates showed multi-drug resistance to antimicrobials. A total of 28 types were detected by enterobacterial repetitive intergenic consensus polymerase chain reaction. Twenty strains had distinct types while 5 types were common for 2 strains and 3 types were common for 3 strains. This is the first current determination of types, clonality and antibiotic resistance of enterovirulent E. coli isolated from small ruminants with diarrhoea. The results of this study showed that the rates of shiga toxigenic, enterotoxigenic and enteropathogenic isolates of E. coli are high in the western part of Turkey. Although these isolates were not clonal, presence of multidrug resistant isolates may cause public health problems.

show abstract

Section: Resultsmentioning

confidence: 99%

Identification, characterization and molecular epidemiology of Escherichia coli isolated from lamb and goat kids with diarrhoea

Türkyılmaz

Eskiizmirliler²,

Tunalıgi̇l³

et al. 2013

Acta Vet. Brno

View full text Add to dashboard Cite

show abstract

“…Trend on the prevalence of E.coli in both diarrhoeic and non diarrhoeic adult pigs and piglets as shown in (Figure 2) The present finding is similar with the findings of Sharma et al, (2004) who also reported highest (94.52%) prevalence of E.coli in comparision to non-diarrhoeic calves. Hussain and Saikia (2000) and Wani et al, (2003) however isolated E.coli from all cases of calf diarrhea. Shah and Jhala (1990) isolated E.coli from 61.2% of diarrhoeic calves.…”

mentioning

confidence: 99%

Prevalence of Escherichia Coli from Pigs and Cattle

Begum¹

2014

J. Anim. Health Prod.

View full text Add to dashboard Cite

Escherichia coli a gram negative rod shaped bacteria has been shown to be normal inhabitant of the gastrointestinal tract of animals and man and most of them are harmless, but some of the serotypes of it are pathogenic and causes diarrhoea and extra intra-intestinal infections in human and animals. A study was done to find out the prevalence of Escherichia coli from pigs and cattle from organized and unorganized farm in Assam. A total of 260 faecal samples, comprising 110 from cattle and 150 from pigs were examined for the presence of E.coli. The overall prevalence of E.coli in cattle was recorded as 67.27%. The prevalence was recorded as 76.67% and 56.00% in diarrhoeic cattle and non-diarrhoeic cattle, respectively. The overall prevalence of E.coli in pigs was recorded as 59.33%. The prevalence was recorded as 70.59% and 44.62% from diarrhoeic pigs and non-diarrhoeic pigs, respectively.All copyrights reserved to Nexus® academic publishers The samples were collected aseptically in sterile vials from organized and unorganized farm of Assam. A total of 260 faecal samples, comprising 110 from cattle and 150 from pigs were examined for the presence of E.coli. All the samples were collected aseptically in sterile vials, immediately brought to the laboratory for bacteriological culture. The commercially available readymade Mac Conkey broth and Mac Conkey lactose agar media (HiMedia, Mumbai) was used for bacterial isolation, identification and characterization of E. coli. Faecal samples were first inoculated in Mac Conkey's broth and incubated at 37°C overnight. For primary isolation of E.coli Mac Conkey lactose agar is used, samples were inoculated on Mac Conkey lactose agar plates and incubated at 37°C overnight. Morphological characteristics like shape, size, arrangement and staining reaction of the organisms of each isolates were studied after staining the fresh culture smears with Gram's stain.The prevalence of E.coli in diarrhoeic and non diarrhoeic adult cattle as well as calves was recorded. A total of 110 faecal samples were collected out of which 74 (67.27%) samples were found positive to E.coli. The prevalence was found to be 60% and 88.57% in diarrhoeic adult cattle and calves, respectively whereas it was found to be 53.33%and 60% in non diarrhoeic cattle and calves, respectively. The overall prevalence of E.coli in diarrhoeic cattle and non-diarrhoeic cattle was found to be 76.67% and 56.00%, respectively. Trend on the prevalence of E. coli in both diarrhoeic and non diarrhoeic adult cattle and calves is shown graphically in (Figure 1).A total of 150 faecal samples from pigs were examined out of which 89 were found to be positive for E.coli.

show abstract

“…Briefly, bacterial strains, grown overnight in nutrient broth at 37 o C, were pelleted by centrifugation at 10000 rpm at 4 o C for 10 min (Hermle Labortechnik GmBH, Germany), and resuspended in 200 µL of sterile distilled water. Bacteria were lysed by boiling for 10 min; lysates were centrifuged as described above and 200 µL of the supernatant were utilized as the polymerase chain reaction (PCR) template [18]. The reaction was performed in a total volume of 25 µL composed of 12.5 µL of EmeraldAmpGT PCR Master Mix, 1 µL F-STa, 1 µL R-STa, 6 STa was produced and purified according to Aref and Saeed [19].…”

Section: Purification and Characterization Of Native Sta From Etecmentioning

confidence: 99%

Novel Heat-Stable Enterotoxin (STa) Immunogen Based on Cationic Nanoliposomes: Preparation, Characterization and Immunization

Aref¹,

Nasr²,

Osman³

2017

J Vaccines Vaccin

View full text Add to dashboard Cite

The novelties encountered in the field of formulation have provided promising solutions for the problematic vaccine development of hapten molecules. In the current study, the novel preparation of a cationic nanoliposomal immunogen of the heat stable enterotoxin (STa) was reported. STa was produced from clinically ETEC isolate of diarrheic neonatal calves and purified using RP-HPLC. STa was loaded into the cationic vesicles which were characterized for their particle size, surface charge, morphology, STa loading, and stability. The STa loaded cationic nanoliposome was used for mice immunization and the generation of STa antibody was monitored using ELISA. Results displayed the spherical nature of the STa loaded vesicles, their suitable size and homogeneity represented by a particle size of 228.1 nm and a PDI of 0.202. The surface charge of the STa nanoliposomes was +29.9, demonstrating sufficient stability during refrigeration storage. The STa loaded cationic nanoliposome was able to elicit specific STa antibody response, and to confer effective protection against STa challenge in mice. The STa antibody binding titer and neutralization capacity were 10 5 and 10 4 mouse units/ml serum, respectively. The developed system is a one-step procedure, which overcomes the disadvantage of the complexity of generation of the hapten-carrier conjugate. In conclusion, the developed STa-cationic nanoliposomal immunogen is feasible and has the potential to improve effectiveness against ETEC, suggesting its applicability in preventing the harmful effects of ETEC infection in neonatal calves.

show abstract

Isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from calves and lambs with diarrhoea in India

Cited by 65 publications

References 23 publications

Identification, characterization and molecular epidemiology of Escherichia coli isolated from lamb and goat kids with diarrhoea

Identification, characterization and molecular epidemiology of Escherichia coli isolated from lamb and goat kids with diarrhoea

Prevalence of Escherichia Coli from Pigs and Cattle

Novel Heat-Stable Enterotoxin (STa) Immunogen Based on Cationic Nanoliposomes: Preparation, Characterization and Immunization

Contact Info

Product

Resources

About