Isolation and Characterization of Stigmasterol and Bis-(5, 7-diacetyl-catechin-4’-α- rhamnopyranoside) from the Stem bark of &lt;i&gt;Neocarya macrophylla&lt;/i&gt; (Sabine) Prance (Chrysobalanaceae)

Yusuf, A. J.; Abdullahi, M. I.; Haruna, Abdurrashid; Idris, Abdullahi; Musa, A. M.

doi:10.4314/njbas.v23i1.3

Cited by 21 publications

(36 citation statements)

References 15 publications

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“…The antimicrobial activity of the methanol stem bark extract of the plant has been reported (Yusuf et al 2015b). Recently, isolation and characterisation of stigmasterol (Figure 1) from the stem bark of N. macrophylla was reported (Yusuf et al 2015a). In this study, antimicrobial activity of stigmasterol isolated from the stem bark of N. macrophylla investigated against representative gram-positive and gram-negative bacteria and two fungi is reported.…”

Section: Introductionmentioning

confidence: 70%

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Antimicrobial activity of stigmasterol from the stem bark of Neocarya macrophylla

Yusuf¹,

Abdullahi²,

Aleku³

et al. 2018

JOMPED

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Background: Natural products play a significant role in human therapy. They represent a huge reservoir of bioactive chemical diversity and help in understanding the cellular pathways that are essential component of drug discovery process. Objective: This study was aimed at evaluating the antimicrobial activity of stigmasterol isolated from the stem bark of Neocarya macrophylla. Methods: Stigmasterol previously isolated from the stem bark of N. macrophylla was subjected to antimicrobial screening against methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), S. aureus, Streptococcus faecalis, Escherichia coli, Salmonella typhimurium, Pseudomonas fluorescens, Klebsiella pneumoniae, Candida albicans and Candida krusei using agar diffusion and broth dilution methods. Results: Susceptibility test results showed that the compound (100 μg/mL) inhibited the growth of all the test organisms with mean zone of inhibition range from 23 mm to 30 mm except the VRE, S. typhi and K. pneumoniae. The activity of stigmasterol was compared with that of ciprofloxacin (5 μg/mL), the standard antibacterial drug, and fluconazole (5 μg/mL), the antifungal agent. The test compound displayed a broad-spectrum of activity, and in many cases exhibited comparable antibacterial activity when compared to ciprofloxacin. Interestingly, the compound also showed antifungal activity against Candida spp., affording comparable inhibitory effect as fluconazole. The minimum inhibitory concentration (MIC) and the minimum bactericidal/fungicidal concentration (MBC/MFC) of stigmasterol range from 6.25 μg/mL to 25 μg/mL and from 12.5 μg/mL to 50 μg/mL, respectively. Conclusion: These properties suggest that the isolated stigmasterol is a potent and broad-spectrum antibacterial and antifungal agent and as such may serve as a lead compound in the development of novel antimicrobial drugs.

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Section: Introductionmentioning

confidence: 70%

“…The structure was established using one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopic analysis and by direct comparison of data obtained with those reported in the literature (Yusuf et al 2015a). …”

Section: Isolation and Structure Elucidation Of Stigmasterolmentioning

confidence: 99%

Antimicrobial activity of stigmasterol from the stem bark of Neocarya macrophylla

Yusuf¹,

Abdullahi²,

Aleku³

et al. 2018

JOMPED

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“…The roots are used in treatment of circumcision wounds, as antivenom and haemostatic agent (Arbonnier, 2004). Stigmasterol and Bis-(5, 7-diacetyl-catechin-4'-α-rhamnopyranoside) were isolated from the stem bark of the plant (Yusuf et al, 2015b).The leaves were reported to have anthelminthic (Barnabas et al, 2010) and antimicrobial (Audu et al, 2005;Halilu et. al., 2010) activities while the stem bark has antimicrobial (Yusuf et al, 2015c), analgesic (Yusuf et al, 2015d) and antivenin activities (Yusuf et al, 2015a).…”

Section: Neocarya Macrophylla (Sabine) Prance Formerlymentioning

confidence: 99%

Isolation of Epicatechin from the Stem Bark of Neocarya macrophylla (Sabine) Prance (Chrysobalanaceae)

Yusuf¹,

Abdullahi²,

Musa³

et al. 2020

Nig J Bas App Sci

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Neocarya macrophylla has a wide range of medicinal uses in traditional medicine. The aim of the study was to isolate and characterize compound from the stem bark of the plant. (-)-Epicatechin (a flavan-3-ol) was isolated from the ethylacetate soluble fraction of the methanol stem bark extract of the plant using a combination of silica gel and sephadex LH-20 column chromatography. The structure of the compound as (-)-epicatechin was confirmed on the basis of chemical test, 1D- & 2D-NMR spectroscopy and comparison with existing data in literature. This is the first report of isolation of epicatechin from the stem bark of the plant. Keywords: Neocarya macrophylla, stem bark, (-)-Epicatechin, NMR analysis

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“…These plants are P. exelsa , P. capensis , P. congenis , P. polyandra , P. campestris , and N. macropylla. N. macrophylla is fairly toxic [ 26 ], and phytochemicals obtained from P. capensis have been found to possess potent antifungal and antimalarial effects [ 27 , 28 ]. P exelsa , P. polyandra , P. congenis , and P. campestris are nontoxic [ 29 – 32 ].…”

Section: Discussionmentioning

confidence: 99%

Determination of the Cytotoxic Effect of Different Leaf Extracts from Parinari curatellifolia (Chrysobalanaceae)

Kundishora

Sithole

Mukanganyama

2020

Journal of Toxicology

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Despite plants being a rich source of useful chemical compounds with different pharmacological properties, some of these compounds may be toxic to humans. Parinari curatellifolia, among its other important pharmacological activities, has been shown to have significant antiproliferative activity on cancer cell lines. Toxicity studies are required to determine the safety profile of P. curatellifolia in the consideration of its potential pharmaceutical benefits as a source of lead compounds in cancer therapy. The effects of P. curatellifolia on both the integrity of the erythrocyte membrane and on normal cells were determined. The dried leaf powder of P. curatellifolia was used in serial exhaustive extraction procedures using hexane, dichloromethane, ethyl acetate, acetone, ethanol, methanol, and water as solvents in addition to extraction using DCM: methanol in equal ratio. Alkaloids, flavonoids, and saponins were isolated from the ethanol extract. The leaf extracts were tested for haemolytic activity on sheep erythrocytes at concentrations of 0.625 to 5 mg/ml. The extracts were also tested for toxicity activity on normal mammalian cells such as the BALB/c mice peritoneal cells using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) at the concentrations of 6.3 to 50 μg/ml. In the haemolysis assays, none of the plant extracts had a significant haemolytic activity with the saponin-enriched extract having the maximum haemolytic activity of 12.2% for a concentration of 5 mg/ml. In the MTT cell viability assay, none of the 11 plant extracts had significant cytotoxicity. The water extract, however, had significant ( p < 0.01 ) proliferative activity towards the murine immune cells at all concentrations. P. curatellifolia leaf extracts were, therefore, not toxic to both erythrocytes and immune cells, and the water extract may have immunostimulatory effects. It is concluded that P. curatellifolia leaf extracts are not toxic in vitro and, therefore, our results support the use of the plant for ethnomedicinal use.

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Isolation and Characterization of Stigmasterol and Bis-(5, 7-diacetyl-catechin-4’-α- rhamnopyranoside) from the Stem bark of <i>Neocarya macrophylla</i> (Sabine) Prance (Chrysobalanaceae)

Cited by 21 publications

References 15 publications

Antimicrobial activity of stigmasterol from the stem bark of <i>Neocarya macrophylla</i>

Antimicrobial activity of stigmasterol from the stem bark of <i>Neocarya macrophylla</i>

Isolation of Epicatechin from the Stem Bark of <i>Neocarya macrophylla</i> (Sabine) Prance (Chrysobalanaceae)

Determination of the Cytotoxic Effect of Different Leaf Extracts from Parinari curatellifolia (Chrysobalanaceae)

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