Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2

Abstract

Bacillus polyfermenticus KJS-2 (BP-KJS-2) was used to produce a lipopeptide-type surfactin. To accomplish this, a surfactin-producing BP-KJS-2 was fermented by soybeans. The surfactin was then purified by a procedure including ethanol treatment and preparative chromatography. Next, the biochemical structure of the purified surfactin was analyzed by electrospray ionization mass spectrometry (ESI-MS) and high-resolution ESI Q-Tof mass spectrometry (Q-Tof MS). In addition, the masses of the four peaks were determined to be 1007, 1021, 1035, and 1049 m/z revealing that the compound was mixture with quasi-molecular ions. Taken together, these findings indicated that the lipopeptide had a cyclic structure and amino acid composition of Gln-Leu-Leu-Leu-Val-Asp-Leu-Leu, and that the major lipopeptide product of BP-KJS-2 is the surfactin isoform. In addition, this lipopeptide showed strong antimicrobial activity against bacteria at the level of 0.05 mg/mL.

Lipopeptide surfactants: Production, recovery and pore forming capacity

“…A variety of cheap raw materials, including plant-derived oils, oil wastes, starchy substances, lactic whey and distillery wastes have been reported to support biosurfactant production. Previous studies reported the use of rice bran [54], soy bean flour [55,56], soybean curd residue [57], potato process effluents [55], potato peels [58,59], sesame peel flour [60], orange peels [61], millet flour [62] tuna fish flour [59], molasses and whey [63], tuna fish cooking residue [60] and soybean sauce [64] for lipopeptide production. Also, numerous studies reported the production of lipopeptide using cassava wastewater as substrate [65][66][67].…”

“…The lipopeptide surfactant Surfactin is well known by its inhibitory activity towards pathogenic bacteria growth [56,133,134]. Other Bacillus related lipopeptides were recognized by their antibacterial activity [18,135,136].…”

Surfactin derivatives from Micromonospora sp. CPCC 202787 and their anti-HIV activities

“…13 Compounds 1-10 were evaluated for their HIV-1 inhibitory activities (Table 2) Surfactins were supposed as characteristic metabolites of the genus Bacillus because they were always isolated from Bacillus spp., especially the Bacillus subtilis. 8,9,[13][14][15][16][17][18][19][20] However, for the first time, surfactins were obtained from Micromonospora sp. strain in this research.…”

Purification and Structural Analysis of Surfactin Produced by Endophytic Bacillus subtilis EBS05 and its Antagonistic Activity Against Rhizoctonia cerealis

“…Surfactins, a major fraction of the B. subtilis antibiotics, are produced as biological control agents to protect cells from attacks by other microorganisms. Specifically, cyclic lipopeptide surfactins play an important role in biological activities, such as antibacterial, antifungal or antiviral activity (Kim et al, 2009). High antifungal activity by endophytic B. subtilis EBS05 is consistent with the known structures of surfactin A isoforms.…”