Isolation and characterization of the immunostimulating β-glucans of an edible mushroom Termitomyces robustus var.

Structural, immunological, and antioxidant studies of β-glucan from edible mushroom Entoloma lividoalbum

“…In the present investigation another water soluble polysaccharide (ELPS) has been isolated from the aqueous extract of this mushroom and characterized as ␤-glucan. Similar type of ␤-glucan was isolated by Bhanja et al (2012) but differing in the number of sugar moieties and linkages in the skeleton chain. The water insoluble ␤-glucan (PS-II) isolated by Bhanja et al (2012) contained (1 → 3)-linked three glucose moieties in the skeleton chain with branching at C-6 by another glucose residue, where as the present material is a water soluble ␤-glucan (ELPS) consisting of five (1 → 3)-, (1 → 6)-linked glucose moieties in the skeleton chain with branching at C-6 by another one.…”

“…Similar type of ␤-glucan was isolated by Bhanja et al (2012) but differing in the number of sugar moieties and linkages in the skeleton chain. The water insoluble ␤-glucan (PS-II) isolated by Bhanja et al (2012) contained (1 → 3)-linked three glucose moieties in the skeleton chain with branching at C-6 by another glucose residue, where as the present material is a water soluble ␤-glucan (ELPS) consisting of five (1 → 3)-, (1 → 6)-linked glucose moieties in the skeleton chain with branching at C-6 by another one. In the present investigation detailed structural characterization, immunological studies and antioxidant properties of the ELPS have been carried out and reported herein.…”

“…Macrophage, splenocyte and thymocyte proliferation assays were determined by adopting the procedures as described previously (Bhanja et al, 2012;Nandi et al, 2013). The formulas to calculate the SPI and TPI are as follows:…”

“…All the chemical shift values of three D residues were same except the chemical shift values of C-6. Among the three D residues, one moiety (D I ) was glycosidically linked to the most rigid part C, hence, its C-6 signal (ı 68.9) showed 0.1 ppm downfield shift with respect to another two residues of D II (ı 68.8) due to neighboring effect (Bhanja et al, 2012) of the rigid part C. The linking at C-6 of the residues C and D were further confirmed by DEPT-135 spectrum (Fig. 2b).…”

Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation

“…Likewise, glucans are involved in various biological processes such as immunostimulation effects, suppressing cancer cells development [8,9] and drug delivery [4]. It was shown that glucans modulated immunes effects regardless of their structural complexity such as β(1 → 3), β(1 → 4) or β(1 → 6) linkages Interestingly, various studies have pointed out that higher degree of structural complexity is coupled with higher immunomodulatory, antioxidant, and anti-cancer effects [3,6,[10][11][12][13][14][15][16]. However these effects are not the only ones.…”