Isolation and Characterization of the Lignans, Isolariciresinol and Pinoresinol, in Flaxseed Meal

Abstract

The isolation and characterization of the lignans, isolariciresinol, pinoresinol, secoisolariciresinol, and matairesinol, potent phytoestrogens, from flaxseed meal are described. This is the first report of isolariciresinol and pinoresinol being detected in a food. The extraction method selected combined the removal of the lignan glycosides from the plant matrix with an alcoholic solvent system, followed by acid hydrolysis to release the aglycons. A reversed-phase high-performance liquid chromatography with diode array detection system was used for initial separation and detection of the lignans at 280 nm in the acid-hydrolyzed methanolic extract. Lignan trimethylsilyl ether derivatives were characterized by gas chromatography/mass spectrometry. Secoisolariciresinol is the major lignan in flaxseed; isolariciresinol, pinoresinol, and matairesinol were identified as minor lignan components.

Protective Effect of a Lignan-Containing Flaxseed Extract against CCl4-Induced Hepatic Injury.

“…Flaxseed is one of the richest sources of plant lignans [15]. In the present study, we showed that pretreatment with flaxseed extract reduced the extent of CCl 4 -induced livernecrosis ( Fig.…”

“…Recently lignans have attracted attention as effective antioxidants in the context of the relationship between diet and degenerative diseases such as cancer and cardiovascular diseases [10,26,31]. In addition to soybean [1], schisandra fruits [9] and whole grains [29], flaxseed is also known as a rich source of plant lignans [15]. Flaxseed contains relatively high amounts of secoisolariciresinol diglucoside (SDG) [17], which has protective effects against hypercholesterolemic arteriosclerosis, diabetes, and metastasis of cancer [12,21,20].…”

Optimization of podophyllotoxin extraction method fromLinum albumcell cultures

“…Alcohol mixed with up to 50% water gives the best blend for lignan extraction. The minimum alcohol concentration should be at least 50%, but preferably 60-80% (Westcott & Muir, 1998;Meagher et al, 1999;Zhang et al, 2007).…”

Combined Effects of Mepiquat Chloride and Trinexapac-ethyl on Oil Content, Lignan, Seed Yield and Endogenous Gibberellins in Flax (Linum usitatissimum L.)

“…Extraction and analysis of lignans including SDG (secoisolariciresinoldiglucoside), SECO (secoisolariciresinol), and ANHSEC (anhydrosecoisolariciresinol) followed the reference (Meagher et al, 1999). Defatted flax powder was extracted with 50 ml of 80% methanol for 5hours at 55℃ in a shaking water bath.…”