Isolation and characterization of the first American bottlenose dolphin papillomavirus: Tursiops truncatus papillomavirus type 2

Rehtanz, Manuela; Ghim, Shin-je; Rector, Annabel; Ranst, Marc Van; Fair, Patricia A.; Bossart, Gregory D.; Jenson, A. Bennett

doi:10.1099/vir.0.82388-0

Cited by 54 publications

(48 citation statements)

References 51 publications

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“…Although papillomavirus has been associated with genital lesions in bottlenose dolphins (Rehtanz et al, 2006), we found no evidence of papillomavirus infection in these genital mucosa lesions. Papillomavirus DNA was not detectable by PCR in any of the samples of the genital mucosa or in the biopsied material from the penile lesion of dolphin 19.…”

Section: Discussioncontrasting

confidence: 44%

“…Because papillomavirus is known to cause genital lesions in bottlenose dolphins (Rehtanz et al, 2006), a papillomavirus PCR was performed on all genital mucosa samples collected in lysis buffer using primers MY11/ MY09 (Manos et al, 1989) and GP5 Roda Husman et al, 1995). This PCR had been successful in detecting DNA of two Phocoena spinnipensis papillomaviruses, one of which is closely related to bottlenose dolphin papillomavirus 2 (Van Bressem et al, 2007).…”

Section: Papillomavirus Pcrmentioning

confidence: 99%

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Genital Herpesvirus in Bottlenose Dolphins (Tursiops Truncatus): Cultivation, Epidemiology, and Associated Pathology

Elk

Bildt

Jong

et al. 2009

Journal of Wildlife Diseases

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ABSTRACT:We studied the pathology, epidemiology, and clinical significance of genital herpesvirus infection in a zoo collection of bottlenose dolphins (Tursiops truncatus). Samples from the genital mucosa of male (n521) and female (n515) dolphins were tested by nested polymerase chain reaction (PCR) targeting the DNA polymerase of herpesvirus. Herpesvirus infection was significantly associated with the occurrence of mucosal plaques on penis (n53) or vulva (n54). Biopsies from a penile plaque showed epithelial hyperplasia by histology, contained herpesvirus-like particles by electron microscopy, and tested positive for herpesvirus by PCR. Herpesvirus was successfully cultivated from penile plaque samples and identified as a member of the Gammaherpesvirinae by DNA sequencing and phylogenetic analysis. We used the newly cultivated bottlenose dolphin herpesvirus (TTHV) to develop a direct enzyme-linked immunosorbent assay for anti-TTHV antibodies in banked sera of these dolphins. The percentage of positive samples was higher in adults (20/21, 95%) than in juveniles (7/15, 47%). Seroconversion occurred around the age of onset of sexual behavior. Although herpesvirus infection has been associated with abortion, perinatal mortality, and urogenital neoplasia in other species, we found no evidence of herpesvirus infection by PCR in tissues from six cases of abortion and perinatal mortality, and no diagnoses of urogenital tumors in 24 bottlenose dolphins from this zoo collection that died since 1990. Together, we here report the first successful cultivation from bottlenose dolphins of a herpesvirus that probably causes benign genital plaques, is endemic in this group of dolphins, and is likely transmitted by sexual contact.

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Section: Discussioncontrasting

confidence: 44%

Section: Papillomavirus Pcrmentioning

confidence: 99%

Genital Herpesvirus in Bottlenose Dolphins (Tursiops Truncatus): Cultivation, Epidemiology, and Associated Pathology

Elk

Bildt

Jong

et al. 2009

Journal of Wildlife Diseases

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“…coelebs and Fringilla montifringilla. 31 To date, there have been no reports of papillomaviruses or any neoplastic lesions in free-ranging seabirds despite the identification of papillomaviruses in numerous marine mammals, 5,35 including dolphins, 36,37 porpoises, 43 and sea turtles. 18 Regardless of host species, productive papillomavirus infection (formation of infectious particles) has been invariably tied to the terminal differentiation of epithelial cells.…”

mentioning

confidence: 99%

Identification of a Novel Papillomavirus in a Northern Fulmar (Fulmarus glacialis) With Viral Production in Cartilage

Gaynor

Fish

Duerr³

et al. 2014

Vet Pathol

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We report the identification of a novel papillomavirus, Fulmarus glacialis papillomavirus 1 (FgPV1), present within an interdigital foot mass of a Northern Fulmar (Fulmarus glacialis). The mass of interest was composed of normal stratified and keratinized epithelium and dense mesenchymal cells with central cartilaginous islands. Within the nuclei of many chondrocytes were loose aggregates or paracrystalline arrays of virions approximately 50 nm in size. Degenerate polymerase chain reaction was used to identify the virus as a putative papillomavirus, and the entire viral genome of 8132 base pairs was subsequently amplified and sequenced. Analysis revealed canonical papillomavirus architecture, including the early open reading frames E6, E7, E1, and E2 and the 2 late proteins L1 and L2. FgPV1 is most closely related to a cluster of avian and reptilian papillomaviruses as visualized by phylogenetic trees. This observation suggests that papillomavirus virion production can occur in mesenchymal cells. Keywords birds, histocytochemistry, chondrocytes, papillomavirus infections, inclusion bodies-viral, transmission electron microscopy, genome-viralThe Papillomaviridae family continues to expand with the ongoing discovery of previously unidentified species occupying over 30 different genera. 46 Avian papillomaviruses have been described in more than 12 avian species and 6 orders 27 since the 1970s. 28,33 In that time, only 3 have been fully sequenced: Francolinus leucoscepus papillomavirus 1 (FlPV1), Psittacus erithacus papillomavirus (PePV), and Fringilla coelebs papillomavirus (FcPV).31,41,44 Among species described so far, avian papillomaviruses and mammalian papillomaviruses share epitheliotropism and similar tissue distribution. In psittacines, virus has been detected in intestinal mucosal cells, 13,16,19,23,27,30,40 nasolacrimal epithelium, 13,16,22 and oral mucosa at the commissure of beaks. 22There is also a report of papillomavirus-associated growths on the legs and feet of two related finch species, Fringilla. coelebs and Fringilla montifringilla. 31 To date, there have been no reports of papillomaviruses or any neoplastic lesions in free-ranging seabirds despite the identification of papillomaviruses in numerous marine mammals, 5,35 including dolphins, 36,37 porpoises, 43 and sea turtles. 18Regardless of host species, productive papillomavirus infection (formation of infectious particles) has been invariably tied to the terminal differentiation of epithelial cells. A small number of papillomaviruses are capable of infecting and transforming fibroblasts, but these infections are considered nonproductive. The members of the Deltapapillomavirus genus such as bovine (Bos taurus) papillomaviruses 1 and 2 (BPV1, BPV2), for example, can infect fibroblasts in cattle, horses, and cats and is associated with transformation in these cells, causing the formation of fibropapillomas, also known as sarcoids in nonbovid species. 20 In these cases, virion production is considered limited to infected cells within the o...

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“…Immunostaining of recombinant baculovirus-infected cells indicated a high level of expression of L1 protein with a homogenous nuclear distribution (data not shown). The recombinant TmPV1 L1 protein showed the ability to self-assemble into empty VLPs, as reported for L1 of all other PVs expressed using a baculovirus system (Kirnbauer et al, 1993;Rose et al, 1993;Rehtanz et al, 2008). Purified VLPs were approximately 55 nm in diameter and had the morphologic appearance of icosahedral particles (Fig.…”

Section: Resultsmentioning

confidence: 72%

“…The TmPV-VLPs are the optimal reagent to measure anti-TmPV1 antibodies resulting from both previous and current infections, whereas DNA detection only reflects the current status (Marais et al, 2000;Stone et al, 2002). In the past, VLP-based ELISAs were successfully used to evaluate PV antibody prevalence in other marine mammal species (Rehtanz et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

SEROEPIDEMIOLOGY OF TmPV1 INFECTION IN CAPTIVE AND WILD FLORIDA MANATEES (TRICHECHUS MANATUS LATIROSTRIS)

Donà

Rehtanz

Adimey

et al. 2011

Journal of Wildlife Diseases

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ABSTRACT:In 1997, cutaneous papillomatosis caused by Florida manatee (Trichechus manatus latirostris [Tm]) papillomavirus 1 (TmPV1) was detected in seven captive manatees at the Homosassa Springs Wildlife State Park, Florida, USA, and, subsequently, in two wild manatees from the adjacent Homosassa River. Since then, papillomatosis has been reported in captive manatees housed in other locations, but not in wild animals. To determine TmPV1 antibody prevalence in captive and wild manatees sampled at various locations throughout Florida coastal regions, virus-like particles, composed of the L1 capsid protein of TmPV1, were generated with a baculovirus expression system and used to measure anti-TmPV1 antibodies in an enzyme-linked immunosorbent assay. Serologic analysis of 156 manatees revealed a TmPV1 antibody prevalence of 26.3%, with no significant difference between captive (n539) and wild (n5117) manatees (28.2% and 25.6%, respectively). No antibody-positive wild animal showed PV-induced cutaneous lesions, whereas papillomatosis was observed in 72.7% of antibody-positive captive manatees. Our data indicate that Florida manatees living in the wild are naturally infected by TmPV1 but rarely show TmPV1-induced papillomatosis. Hence, it appears that the wild population would not be harmed in a case of contact with captive animals without visible lesions and productive infections, which could be thus released into the wild.

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Isolation and characterization of the first American bottlenose dolphin papillomavirus: Tursiops truncatus papillomavirus type 2

Cited by 54 publications

References 51 publications

Genital Herpesvirus in Bottlenose Dolphins (Tursiops Truncatus): Cultivation, Epidemiology, and Associated Pathology

Genital Herpesvirus in Bottlenose Dolphins (Tursiops Truncatus): Cultivation, Epidemiology, and Associated Pathology

Identification of a Novel Papillomavirus in a Northern Fulmar (Fulmarus glacialis) With Viral Production in Cartilage

SEROEPIDEMIOLOGY OF TmPV1 INFECTION IN CAPTIVE AND WILD FLORIDA MANATEES (TRICHECHUS MANATUS LATIROSTRIS)

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