Isolation and characterization of the equol-producing bacterium Slackia sp. strain NATTS

Tsuji, Hirokazu; Moriyama, Kaoru; Nomoto, Koji; Miyanaga, Naoto; Akaza, Hideyuki

doi:10.1007/s00203-010-0546-z

Cited by 69 publications

(66 citation statements)

References 37 publications

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“…In the present study, detection proportions of subjects for Slackia sp. strain NATTS in cases and controls were smaller than these percentages shown by Tsuji et al (2010).…”

Section: Discussioncontrasting

confidence: 59%

“…strain NATTS have a potent equol-producing ability from daidzein. Tsuji et al (2010) reported that the detection rate of Slackia sp. strain NATTS in the feces of 40 healthy volunteers were 33% in subjects under 40 years old and were 47% in subjects over 40 years old.…”

Section: Discussionmentioning

confidence: 99%

“…The 16S rRNA gene fragments of Slackia sp. strain NATTS and related strains were amplified from the total RNA extracted from the fecal samples in order to determine the number of species by RT-qPCR (reverse transcription-quantitative PCR) (Tsuji et al, 2010). Detection limits were less than 0.5ng/ml for all isoflavones concentrations and 3.9 log 10 cell/g for counts of Slackia sp.…”

Section: Study Population/designmentioning

confidence: 99%

“…Slackia sp. strain NATTS was found to transform daidzein into equol, and belong to genus Slackia family Coriobacteriaceae by 16S rRNA gene sequence-based analysis (Tsuji et al, 2010). Tsuji et al (2012) showed that, in Slackia sp.…”

Section: Introductionmentioning

confidence: 99%

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Counts of Slackia sp. strain NATTS in Intestinal Flora are Correlated to Serum Concentrations of Equol both in Prostate Cancer Cases and Controls in Japanese Men

Sugiyama¹,

Nagata²,

Fukuta³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Isoflavones, which are included in soybeans, have been suggested to protect against prostate cancer. Equol, one of isoflavones, is an intestinally derived bacterial metabolite of daidzein. A newly identified equol-producing bacterium, Slackia sp. strain NATTS, with a high equol-producing activity was isolated from human feces in Japanese adults. Counts of Slackia sp. strain NATTS in intestinal flora have not been assessed with regard to prostate cancer risk. In this study, we investigated the association of serum isoflavones and counts of Slackia sp. strain NATTS with prostate cancer risk in a case-control study. Materials and Methods: Concentrations of isoflavones and counts of Slackia sp. strain NATTS in feces were measured from 44 patients with prostate cancer and 28 hospital controls. The risk of prostate cancer was evaluated in terms of odds ratios (ORs) and 95% confidence intervals (CIs) by the logistic regression analysis. Results: The detection proportions of Slackia sp. strain NATTS in cases and controls were 34.1% and 25.0%, respectively. Counts of Slackia sp. strain NATTS were significantly correlated with serum concentrations of equol both in cases and controls (Spearman correlation coefficients, r s =0.639 and r s =0.572, p<0.01, respectively). Serum concentrations of genistein, daidzein, glycitein, and equol were not significantly associated with risk of prostate cancer. Conclusions: This study found that counts of Slackia sp. strain NATTS correlated with serum concentrations of equol both in prostate cancer cases and controls, but serum isoflavone concentrations were not associated with risk of prostate cancer in our patients.

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“…In the present study, detection proportions of subjects for Slackia sp. strain NATTS in cases and controls were smaller than these percentages shown by Tsuji et al (2010).…”

Section: Discussioncontrasting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Study Population/designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Counts of Slackia sp. strain NATTS in Intestinal Flora are Correlated to Serum Concentrations of Equol both in Prostate Cancer Cases and Controls in Japanese Men

Sugiyama¹,

Nagata²,

Fukuta³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…strain NATTS, which has potent daidzein-to-equol conversion ability, from healthy human feces (26). This strain has a more potent daidzein-equol conversion activity than the other equol-producing strains previously reported (26).…”

mentioning

confidence: 87%

Identification of an Enzyme System for Daidzein-to-Equol Conversion in Slackia sp. Strain NATTS

Tsuji

Moriyama

Nomoto

et al. 2012

Appl Environ Microbiol

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An Escherichia coli library comprising 8,424 strains incorporating gene fragments of the equol-producing bacterium Slackia sp. strain NATTS was constructed and screened for E. coli strains having daidzein-and dihydrodaidzein (DHD)-metabolizing activity. We obtained 3 clones that functioned to convert daidzein to DHD and 2 clones that converted DHD to equol. We then sequenced the gene fragments inserted into plasmids contained by these 5 clones. All of the gene fragments were contiguous, encoding three open reading frames (ORF-1, -2, and -3). Analysis of E. coli strains containing an expression vector incorporating one of the orf-1, -2, or -3 genes revealed that (i) the protein encoded by orf-1 was involved in the conversion of cis/trans-tetrahydrodaidzein (cis/trans-THD) to equol, (ii) the protein encoded by orf-2 was involved in the conversion of DHD to cis/trans-THD, and (iii) the protein encoded by orf-3 was involved in the conversion of daidzein to DHD. ORF-1 had a primary amino acid structure similar to that of succinate dehydrogenase. ORF-2 was presumed to be an enzyme belonging to the short-chain dehydrogenase/reductase superfamily. ORF-3 was predicted to have 42% identity to the daidzein reductase of Lactococcus strain 20-92 and belonged to the NADH:flavin oxidoreductase family. These findings showed that the daidzein-to-equol conversion reaction in the Slackia sp. NATTS strain proceeds by the action of these three enzymes.S oybean isoflavones and their derivatives have been reported to prevent sex hormone-dependent diseases, such as prostate cancer, breast cancer, menopausal disorders, premenstrual syndrome, and osteoporosis (3,9,10,16,21,30). The isoflavone equol is expected to prevent hormone-dependent diseases, such as prostate cancer, because of its ability to bind to dihydrotestosterone and its high capacity to bind to estrogen receptor ␤; moreover, it is the most potent antioxidant of all the isoflavones (1, 2, 5, 17, 23).To date, several bacteria capable of producing equol have been isolated from human or animal feces (18-20, 29, 31). Many of these strains are suggested to first metabolize daidzein as a substrate to dihydrodaidzein (DHD) and to then metabolize DHD to equol. Recently, daidzein reductase, which converts daidzein to DHD, has been purified from the equol-producing Lactococcus strain 20-92 (25). On the other hand, it has been suggested that, in the Eggerthella strain Julong 732, DHD is converted to equol by the production of cis/trans-tetrahydrodaidzein (cis/trans-THD) as an intermediate metabolite (13,14). These studies have therefore suggested that daidzein is converted to equol via DHD and cis/ trans-THD. However, the details of the enzymes involved in the production of equol from daidzein, and of the genes encoding them, remain largely unknown.We have recently isolated Slackia sp. strain NATTS, which has potent daidzein-to-equol conversion ability, from healthy human feces (26). This strain has a more potent daidzein-equol conversion activity than the other equol-producing strains...

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Understanding Polyphenols' Health Effects Through the Gut Microbiota

Selma

Tomás‐Barberán

Romo‐Vaquero

et al. 2020

Dietary Polyphenols

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Isolation and characterization of the equol-producing bacterium Slackia sp. strain NATTS

Cited by 69 publications

References 37 publications

Counts of Slackia sp. strain NATTS in Intestinal Flora are Correlated to Serum Concentrations of Equol both in Prostate Cancer Cases and Controls in Japanese Men

Counts of Slackia sp. strain NATTS in Intestinal Flora are Correlated to Serum Concentrations of Equol both in Prostate Cancer Cases and Controls in Japanese Men

Identification of an Enzyme System for Daidzein-to-Equol Conversion in Slackia sp. Strain NATTS

Understanding Polyphenols' Health Effects Through the Gut Microbiota

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