Isolation and Characterization of Three MADS-box Genes from Alpinia hainanensis (Zingiberaceae)

Song, Jun; Ma, Wei; Yuanjiang, Tang; Chen, Zhongyi; Liao, Jingping

doi:10.1007/s11105-009-0147-7

Cited by 16 publications

(18 citation statements)

References 63 publications

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“…As implied by the name, petaloid staminodes are thought to have evolved from stamens. Recently, many efforts have been made to reveal the molecular basis for androecial petaloidy of Zingiberales species including C. indica , yet the exact mechanism remains unclear (Bartlett and Specht, 2010 ; Song et al, 2010 ; Almeida et al, 2013 ; Yockteng et al, 2013 ; Fu et al, 2014 ; Almeida et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Tian

Liu

et al. 2016

Front. Plant Sci.

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Canna indica (Zingiberales) is one of the most important ornamental species characterized with beautiful petaloid staminodes, which are considered to evolve from stamens. However, the genetic basis for the development of petaloid staminodes remains unclear largely because the genomic sequences are not available. By using RNA-Seq, we sequenced the transcripts in the flower of C. indica, and quantified the temporal gene expressions in flower primordium and differentiated flower, as well as the spatial gene expressions in petal and petaloid staminode. In total, 118,869 unigenes were assembled, among which 67,299 unigenes were annotated. Quantification analysis identified the differentially expressed genes in the temporal and spatial two comparisons, based on which, Gene Ontology enrichment analysis highlighted the representative terms in each sample, such as specification of organ number in flower primordium, growth in differentiated flower, secondary cell wall biogenesis in petal and cell division in petaloid staminode. Among the 51 analyzed MADS-box unigenes, 37 were up-regulated in differentiated flower compared with those in flower primordium. A-class unigenes were expressed higher in petal than in petaloid staminode, and C-class unigenes were expressed oppositely, whereas B-class unigenes demonstrated close expression levels in these two organs, indicating that petaloid staminode retains stamen identity to some degree. In situ hybridization provided more detailed expression patterns of these unigenes, and revealed the extended expression of B-class to the carpel at later stages when the style turned flat. These results constitute a preliminary basis for the study of flower development in C. indica and can be applied in further study of the evolution of Zingiberales.

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Section: Introductionmentioning

confidence: 99%

Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Tian

Liu

et al. 2016

Front. Plant Sci.

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“…5′ and 3′ rapid amplification of cDNA ends (RACE) were used to isolate the full–length cDNA of AhSEP3b genes with degenerate primers, following the SMARTer RACE cDNA Amplification Kit User Manual of Clontech (Takara Bio Company). The other three genes ( AhFUL , AhSEP4 , and AhAGL6–like ) were isolated as previously described [65] . Quantitative RT–PCR (qRT–PCR) was performed with gene–specific primers, using the 18S primer as an internal control.…”

Section: Methodsmentioning

confidence: 99%

Functional Conservation and Divergence of Four Ginger AP1/AGL9 MADS–Box Genes Revealed by Analysis of Their Expression and Protein–Protein Interaction, and Ectopic Expression of AhFUL Gene in Arabidopsis

Fan

Song

et al. 2014

PLoS ONE

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Alpinia genus are known generally as ginger–lilies for showy flowers in the ginger family, Zingiberaceae, and their floral morphology diverges from typical monocotyledon flowers. However, little is known about the functions of ginger MADS–box genes in floral identity. In this study, four AP1/AGL9 MADS–box genes were cloned from Alpinia hainanensis, and protein–protein interactions (PPIs) and roles of the four genes in floral homeotic conversion and in floral evolution are surveyed for the first time. AhFUL is clustered to the AP1lineage, AhSEP4 and AhSEP3b to the SEP lineage, and AhAGL6–like to the AGL6 lineage. The four genes showed conserved and divergent expression patterns, and their encoded proteins were localized in the nucleus. Seven combinations of PPI (AhFUL–AhSEP4, AhFUL–AhAGL6–like, AhFUL–AhSEP3b, AhSEP4–AhAGL6–like, AhSEP4–AhSEP3b, AhAGL6–like–AhSEP3b, and AhSEP3b–AhSEP3b) were detected, and the PPI patterns in the AP1/AGL9 lineage revealed that five of the 10 possible combinations are conserved and three are variable, while conclusions cannot yet be made regarding the other two. Ectopic expression of AhFUL in Arabidopsis thaliana led to early flowering and floral organ homeotic conversion to sepal–like or leaf–like. Therefore, we conclude that the four A. hainanensis AP1/AGL9 genes show functional conservation and divergence in the floral identity from other MADS–box genes.

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“…1). These domains are involved in transcriptional activation or the formation of multimeric transcription factor complexes (Cho et al 1999;Egea-Cortines et al 1999;Song et al 2010). Key structural features, such as a potential phosphorylation site for calmodulin-dependent protein kinases (QVTFS) within a nuclear localization signal, were also identified in the MADS-box domain (Fig.…”

Section: Bpmads Bioinformatics Analysismentioning

confidence: 99%

Molecular Cloning and Expression Analysis of 13 MADS-Box Genes in Betula platyphylla

Liu

Wang

et al. 2011

Plant Mol Biol Rep

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MADS-box genes encode a family of transcription factors that control a diverse range of processes in flowering plants. In this study, 13 unique MADS genes were cloned from Betula platyphylla Suk., and 2-year-old Betula seedlings propagated in glasshouses were selected as plant materials. The expression profile of each BpMADS was investigated during the growth season and following gibberellin (GA) treatments by real-time quantitative reverse transcription polymerase chain reaction. The relative abundance of the 13 BpMADS was shown to differ during each month, indicating that the activity of the genes varies during the annual growing period. Expression analyses demonstrated that these BpMADS were regulated by GA signaling pathways. Furthermore, the variations in expression patterns suggest that the genes act independently to fulfill specific functions or act cooperatively in physiological processes. The study of birch MADSs is important for the purposes of improving breeding techniques and molecular biotechnology.

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Isolation and Characterization of Three MADS-box Genes from Alpinia hainanensis (Zingiberaceae)

Cited by 16 publications

References 63 publications

Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Functional Conservation and Divergence of Four Ginger AP1/AGL9 MADS–Box Genes Revealed by Analysis of Their Expression and Protein–Protein Interaction, and Ectopic Expression of AhFUL Gene in Arabidopsis

Molecular Cloning and Expression Analysis of 13 MADS-Box Genes in Betula platyphylla

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