Isolation and Characterization of Three Zygomycetous Fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus

Nguyen, Thuong T. T.; Lee, Hyang Burm

doi:10.1080/12298093.2018.1538071

Cited by 12 publications

(7 citation statements)

References 40 publications

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“…Cultural characteristics such as colony color, colony reverse pigmentation, texture, and shape were observed and documented. All isolates were also examined under a light microscope for microscopic details (Optika, Italy) [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

Production and characterization of melanin pigment from black fungus Curvularia soli AS21 ON076460 assisted gamma rays for promising medical uses

Abd-EL-Aziz,

Abed,

Mahfouz

et al. 2024

Microb Cell Fact

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Owing to the growing need for natural materials in different fields, studying melanin production from biological sources is imperative. In the current study, the extracellular melanin pigment was produced by the fungus Curvularia soli AS21 ON076460. The factors that affect the production of melanin were optimized by the Plackett-Burman design (P-BD). The effect of gamma irradiation on melanin productivity was investigated. The maximum melanin yield (3.376 mg/L) was elicited by a stimulus of gamma irradiation at 1.0 kGy. The results evoked that, Curvularia soli AS21 ON076460 melanin exhibited excellent antimicrobial activity against all tested bacteria and fungi. Klebsiella pneumoniae ATCC 13883 and P. digitatum were mostly affected by melanin registering the inhibition zone diameters of 37.51 ± 0.012 and 44.25 ± 0.214 mm, respectively. Moreover, Curvularia soli AS21 ON076460 melanin indicated a significant antiviral efficacy (77% inhibition) of Herpes simplex virus (HSV1). The melanin pigment showed antioxidant activities with IC50 of 42 ± 0.021 and 17 ± 0.02 µg/mL against DPPH and NO, respectively. Melanin had cytotoxic action against human breast cancer and skin cancer cell lines (Mcf7and A431) as well as exerting a low percentage of cell death against normal skin cell lines (Hfb4). Melanin was effective in wound management of human skin cells by 63.04 ± 1.83% compared with control (68.67 ± 1.10%). The novelty in the study is attributed to the possibility of using gamma rays as a safe method in small economic doses to stimulate melanin production from the fungi that have been isolated. In summary, melanin produced from fungi has significant biological activities that encourage its usage as a supportive medical route.

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Section: Methodsmentioning

confidence: 99%

Production and characterization of melanin pigment from black fungus Curvularia soli AS21 ON076460 assisted gamma rays for promising medical uses

Abd-EL-Aziz,

Abed,

Mahfouz

et al. 2024

Microb Cell Fact

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“…For by-products of rice bran and water samples, we used the serial dilution plating method as described by Nguyen and Lee [28] and Nguyen et al [29]. Individual colonies with various morphologies were collected, transferred to PDA, and subcultured until pure mycelia were obtained.…”

Section: Sampling and Isolationmentioning

confidence: 99%

Five New Records of the Family Aspergillaceae in Korea, Aspergillus europaeus, A. pragensis, A. tennesseensis, Penicillium fluviserpens, and P. scabrosum

et al. 2020

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During an investigation of the fungi from the Aspergillaceae family obtained from different environmental sources in Korea, we isolated six strains, including CNUFC WJC9-1, CNUFC BPM36-33, CNUFC MSW6, CNUFC ESW1, CNUFC TM6-2, and CNUFC WD17-1. The morphology and phylogeny of these isolates were analyzed based on their partial b-tubulin (BenA) and calmodulin (CaM) gene sequences. Based on the morphological characteristics and sequence analyses, the isolates CNUFC WJC9-1, CNUFC BPM36-33, CNUFC TM6-2, and CNUFC WD17-1 were identified as A. europaeus, A. pragensis, Penicillium fluviserpens, and P. scabrosum, respectively, and isolates CNUFC MSW6 and CNUFC ESW1 were identified as A. tennesseensis. To the best of our knowledge, the species A. europaeus, A. pragensis, A. tennesseensis, P. fluviserpens, and P. scabrosum have not been previously reported in Korea.

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“…Members of Backusella , the unique genus of Backusellaceae, have been retrieved from soil, leaf litter, dung, moist wall, agarics, decaying wood, Fragaria , diseased roots, and Medicago sativa [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 ]. However, none of the Backusella species were isolated from invertebrates and toads.…”

Section: Introductionmentioning

confidence: 99%

“…circina and B . locustae , have been described [ 8 , 11 ]. Thus, many Backusella species still need to be investigated in Korea.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Novel Backusella (Backusellaceae, Mucorales) Isolated from Invertebrates and Toads in Cheongyang, Korea

Nguyen

Voigt

Santiago

et al. 2021

JoF

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Three novel fungal species, Backusella chlamydospora sp. nov., B. koreana sp. nov., and B. thermophila sp. nov., as well as two new records, B. oblongielliptica and B. oblongispora, were found in Cheongyang, Korea, during an investigation of fungal species from invertebrates and toads. All species are described here using morphological characters and sequence data from internal transcribed spacer sequences of ribosomal DNA and large subunit of the ribosomal DNA. Backusella chlamydospora is different from other Backusella species by producing chlamydospores. Backusella koreana can be distinguished from other Backusella species by producing abundant yeast-like cells. Backusella thermophila is characterized by a variable (subglobose to oblong, applanate to oval, conical and ellipsoidal to pyriform) columellae and grows well at 37 °C. Multigene phylogenetic analyses of the combined ITS and LSU rDNA sequences data generated from maximum likelihood and MrBayes analyses indicate that B. chlamydospora, B. koreana, and B. thermophila form distinct lineages in the family Backusellaceae. Detailed descriptions, illustrations, phylogenetic tree, and taxonomic key to the Backusella species present in Korea are provided.

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Isolation and Characterization of Three Zygomycetous Fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus

Cited by 12 publications

References 40 publications

Production and characterization of melanin pigment from black fungus Curvularia soli AS21 ON076460 assisted gamma rays for promising medical uses

Production and characterization of melanin pigment from black fungus Curvularia soli AS21 ON076460 assisted gamma rays for promising medical uses

Five New Records of the Family Aspergillaceae in Korea, Aspergillus europaeus, A. pragensis, A. tennesseensis, Penicillium fluviserpens, and P. scabrosum

Discovery of Novel Backusella (Backusellaceae, Mucorales) Isolated from Invertebrates and Toads in Cheongyang, Korea

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