Isolation and Characterization of Trichoderma spp. for Antagonistic Activity Against Root Rot and Foliar Pathogens

Kumar, Krishna; Amaresan, N.; Bhagat, Someshwar; Madhuri, K.; Srivastava, R. C.

doi:10.1007/s12088-011-0205-3

Cited by 83 publications

(62 citation statements)

References 18 publications

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“…In vitro assay clearly showed that antagonists T. harzianum halted the radial mycelium growth of highly virulent isolate F. oxysporum, R. solani and S. rolfsii, with varying levels of antagonism. Similar antagonistic effect of T. harzianum against these soil-borne pathogens infecting many other crops was also observed by several other investigators (Sundar et (Kumar et al, 2012), and there may be relationship between the production of these enzymes and the ability to inhibit the pathogen (Elad et al, 1982, Sivan andChet, 1989). Results in this study also revealed that the radial mycelial growth of the pathogens was interfered by T. harzianum within the contact area or interface zone.…”

Section: IVsupporting

confidence: 89%

Effect of Trichoderma Fortified Compost on Disease Suppression, Growth and Yield of Chickpea

Talukdar¹,

Siddiqa²,

Masum³

et al. 2017

IJEAB

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Section: IVsupporting

confidence: 89%

Effect of Trichoderma Fortified Compost on Disease Suppression, Growth and Yield of Chickpea

Talukdar¹,

Siddiqa²,

Masum³

et al. 2017

IJEAB

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“…Em conformidade com o nosso estudo, Adandonon et al (2006) Nesse contexto, uma possível explicação para o alto antagonismo de CEN155, CEN158, CEN169, CEN170, CEN194 e CEN197 contra o fungo S. rolfsii, em detrimento dos demais isolados de T. harzianum, reside no fato de que o conjunto de mecanismos de controle biológico anteriormente citado, pode variar entre isolados de uma mesma espécie, tanto em experimentos in vitro (KUMAR et al, 2012) quanto em experimentos in vivo (MIRANDA et al, 2006). Segundo Louzada et al (2009), não há relatos na literatura que informem sobre a perda de diversidade de Trichoderma spp.…”

Section: Resultsunclassified

“…em condições de campo consiste em prática promissora, uma vez que o controle biológico oferece maior durabilidade, segurança e melhor custo-efetividade do que os fungicidas químicos aplicados no solo (YAQUB; SHAHZAD, 2011;KUMAR et al, 2012). Os agricultores podem usar isolados de Trichoderma efetivos contra S. rolfsii, almejando reduzir perdas no estande inicial e na produção das culturas do feijoeiro e soja, e como conseqüência, incrementar os rendimentos.…”

Section: Resultsunclassified

“…Os fungos do gênero Trichoderma (teleomorfo Hypocrea) são oportunistas, simbiontes de plantas, fortes competidores no ambiente do solo, constituem fontes de enzimas degradadoras de parede de outros fungos, são, também, importantes produtores de antibióticos e parasitas de fungos fitopatogênicos (KUMAR et al, 2012). Tais características são fundamentais para o sucesso desses organismos como agentes de biocontrole (LORITO et al, 1993).…”

Section: Introductionunclassified

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Antagonismo de Trichoderma harzianum a Sclerotium rolfsii nas culturas do feijoeiro e soja.

2013

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Resumo -Os fungicidas sintéticos embora sejam eficientes no controle de fungo fitopatogênicos, apresentam potencial efeito tóxico à saúde humana e ao meio ambiente. Nesse contexto, o controle biológico tem sido utilizado como alternativa para reduzir os prejuízos causados por Sclerotium rolfsii em diversas culturas de importância econômica no Brasil. Sendo assim, objetivou-se com este trabalho estudar o potencial de 23 isolados de Trichoderma harzianum provenientes de solo de Cerrado como agente de controle biológico de S. rolfsii, importante patógeno nas culturas do feijoeiro e soja. A ação antagonista dos isolados foi verificada por meio do pareamento de culturas sob três temperaturas (22ºC, 25ºC e 28ºC) e em experimentos conduzidos em casa de vegetação, com as culturas de feijoeiro e soja. Os isolados CEN155, CEN158, CEN169, CEN170, CEN194 e CEN197 destacaram-se quanto à inibição do crescimento micelial de S. rolfsii, nas três temperaturas empregadas nos experimentos in vitro, ocupando pelo menos 66% da superfície do meio em placa de Petri (90 mm) e também na supressão in vivo de S. rolfsii nos experimentos com as duas culturas em casa de vegetação, proporcionando percentual médio de plantas sadias acima de 88%, aos 15 dias após as inoculações de patógeno e antagonistas no solo. Palavras-chave -Glycine max. Phaseolus vulgaris. Podridão radicular. Controle biológico.Abstract -Although synthetic fungicides have been efficient for controlling phytopathogenic fungi, these products present potential toxic effect to the human health and to environment. In this context, the biological control has been used as an alternative to reduce losses caused by Sclerotium rolfsii in several crops of economic importance in Brazil. Therefore, the objective of this work was to study 23 isolates of Trichoderma harzianum obtained from Cerrado soils as biological control agent against S. rolfsii, an important soil-borne pathogen of bean and soybean crops. The antagonism of the isolates was verified by dual culture assays (at 22ºC, 25ºC and 28ºC) and in vivo experiments carried out in greenhouse, using common bean and soybean plants. The isolates CEN155, CEN158, CEN169, CEN170, CEN194 and CEN197 were able to inhibit the mycelial growth of S. rolfsii in the three temperatures employed in the dual culture assays, colonizing at least 66% of the Petri dish surfaces and were also effective for the in vivo controlling of S. rolfsii on bean and soybean crops, affording average value (%) of healthy plants over 88%, at 15 days after soil inoculations with pathogen and antagonists.

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“…Dentre as espécies, T. harzianum e T. asperellum são as mais estudadas, além de outras como T. koningii, T. viride, T. hamatum e T. pseudokoningii. As espécies desse gênero produzem enzimas líticas que causam degradação das paredes celulares dos fungos que hiperparasitam, sendo a produção dessas enzimas diretamente relacionada com a eficácia no processo de hiperparasitismo (KUMAR et al, 2012).…”

Section: Survival Of Resistance Structures Inunclassified

Sobrevivência de estrutura de resistência de Macrophomina phaseolina e Sclerotium rolfsii em solo tratado biologicamente

Nascimento

Silva

Cruz

et al. 2016

AGRO@MBIENTE ON-LINE

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. An evaluation of antagonism was determined from the score allotted to the percentage of growth of isolates of Trichoderma spp. in relation to the phyto-pathogenic fungi, as well as the percentage inhibition in pathogen growth in relation to the Compost Aid ® product. The survival of resistance structures in the pathogenic fungi was evaluated 40 days after application of the treatments by plating onto a semiselective culture medium and BDA. For the experiment in vitro, all the treatments with Trichoderma produced a percentage inhibition greater than 50% for both phyto-pathogenic fungi. The commercial products Tricobiol ® and Triconemate ® gave the greatest percentage inhibition for the fungus S. rolfsii (62.5%). The Compost Aid ® product gave 100% and 98.57% inhibition in the growth of the fungi M. phaseolin and S. rolfsii respectively. The treatments in the form of a mixture of Trichoderma and Tricobiol ® had a median value of 100%, while Triconemate ® resulted in a 96% inhibition in the growth of the microsclerotia of M. phaseolina recovered from the soil; however none of these treatments inhibited sclerotial germination in S. rolfsii. The product Compost Aid ® resulted in a median of 100% and 0% growth in resistance structures for the M. phaseolina and S. rolfsii fungi respectively.

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Isolation and Characterization of Trichoderma spp. for Antagonistic Activity Against Root Rot and Foliar Pathogens

Cited by 83 publications

References 18 publications

Effect of Trichoderma Fortified Compost on Disease Suppression, Growth and Yield of Chickpea

Effect of Trichoderma Fortified Compost on Disease Suppression, Growth and Yield of Chickpea

Antagonismo de Trichoderma harzianum a Sclerotium rolfsii nas culturas do feijoeiro e soja.

Sobrevivência de estrutura de resistência de Macrophomina phaseolina e Sclerotium rolfsii em solo tratado biologicamente

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