Isolation and characterization of two homeodomain leucine zipper genes from the dioecious plant Silene latifolia.

Ageez, Amr; Matsunaga, Sachihiro; Uchida, Wakana; Sugiyama, Ryuji; Kazama, Yusuke; Kawano, Shigeyuki

doi:10.1266/ggs.78.353

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…At stage 10, transcripts of neither SlSEP1 nor SlSEP3 were detected in the suppressed organs. This suppression is consistent with the behavior of other MADS-box genes (Hardenack et al 1994;Matsunaga et al 2003), a homeodomain leucine zipper gene (Ageez et al 2003) and cell-cycle specific genes (Matsunaga et al 2004), which are not expressed in the suppressed organs. Transcripts of both genes continued to be expressed in developing petals of male and female flower buds.…”

Section: Resultssupporting

confidence: 81%

Characterization of two SEPALLATA MADS-box genes from the dioecious plant Silene latifolia

et al. 2004

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Two SEPALLATA orthologs, SlSEP1 and SlSEP3, were isolated from male flower buds of the dioecious plant Silene latifolia. Both genes are located on autosomes and not on the sex chromosomes. SlSEP1 and SlSEP3 transcripts were detected specifically in male and female flower buds. Quantitative RT-PCR and in situ hybridization revealed that both genes were expressed in young flower meristems, developing petals, male anthers, and female ovules. However, neither transcript was detected in suppressed gynoecia of male flower buds or suppressed anthers of female flower buds. The genes were differentially expressed during anther development, with the SlSEP1 transcript accumulating in anther walls and tapetal cells, and the SlSEP3 transcript accumulating in tetrads as well as anther walls and tapeta. Transcripts of both genes were also detected in flower buds of mutants with deletions of some parts of the Y chromosome, suggesting that neither gene is directly involved in sex determination.

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Section: Resultssupporting

confidence: 81%

Characterization of two SEPALLATA MADS-box genes from the dioecious plant Silene latifolia

et al. 2004

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“…These homeobox genes are involved in a wide range of process in plants and are expressed in different organs and developmental stages. Their expression patterns are modified by processes causing developmental responses like light, hormones, stress and wounding (Schena et al, 1993;Aoyama et al, 1995;Baima et al, 1995;Carabelli et al, 1996;Söderman et al, 1996;Aso et al, 1999;Ingram et al, 2000;Sakakibara et al, 2001;Ageez et al, 2003). Eucalyptus HD-Zip I genes were also present in almost all gene expression groups (Figure 3), but the greatest number of ESTcontigs (seven out of 19) were observed in expression groups V and VI (stem from drought stress susceptible seedlings).…”

Section: Expression Patternmentioning

confidence: 95%

“…They are separated into four different families (HD-Zip I, II, III and IV) depending on specific differences of the leucine zipper motif (Chan et al, 1998;Aso et al, 1999;Ingram et al, 2000;Sakakibara et al, 2001;Ageez et al, 2003). This kind of homeodomain organization is present only in plants and it is speculated that HD-Zip genes originated in plant lineage by exon exchange between a homeodomain gene and a leucine zipper containing sequence (Schena and Davis, 1992).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of homeobox genes in Eucalyptus

et al. 2005

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Homeobox genes encode transcriptional factors, usually involved in molecular control of plant developmental patterns. They can be divided into several classes according to conserved sequences within the homeobox region and the presence of specific additional sequences. Based on these conserved sequences, we developed a search procedure to identify possible homeobox genes in the Eucalyptus Genome Sequencing Project Consortium (FORESTs) database. We were able to identify 50 Eucalyptus sequences (EST-contigs) containing the homeodomain sequence. Phylogenetic analysis was applied to this ESTs-contigs and 44 of them were found to have similarities with one of three well-known homeobox classes: Bell, Knox and HD-Zip, and their sub-classes. However, no EST-contig grouped with the fourth important homeobox class, the PHD-finger homeobox. On the other hand, two sequences have showed pronounced similarity to the Arabidopsis thaliana Wuschel gene, considered an "atypical" homeobox gene. Hierarchical clustering analysis of the expression pattern of these putative Eucalyptus homeobox genes revealed the presence of ten distinct expression groups. Combining phylogenetic analysis and expression patterns for some of the Eucalyptus genes revealed interesting aspects about some of the potential homeobox genes, which might lead to a better understanding of the Eucalyptus biology and to biotechnological applications.

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“…Reverse transcription-polymerase chain reaction (RT-PCR) and cloning were performed as described elsewhere ). An S. latifolia male flower bud full-length cDNA library was screened using a cloned ASK1-like gene fragment as a probe, as described elsewhere (Ageez et al 2003, Matsunaga et al 2004. For DNA gel blot analysis and in-situ hybridization, 3′ and 5′ untranslated regions (UTR) were amplified by PCR and cloned into a pGEM-T easy vector (Promega, WI, USA).…”

Section: Isolation Of Cdnas Encoding Ask1-like Proteinmentioning

confidence: 99%

Expression of ASK1-like genes in arrested stamens of female Silene latifolia plants

2006

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Silene latifolia is a dioecious plant in which sex is determined by heteromorphic sex chromosomes. In female plants, stamen development is arrested before microspore mother cells are formed. In this study, we isolated four cDNAs (SlSKP1-1 to 4) encoding ASK1-like protein as expression markers to reveal when expression levels are reduced in arrested stamens of female flowers. Expression patterns of the SlSKP1 genes were analyzed by in-situ hybridization. We use the flower development classification of Grant et al. (in Plant J 6:471-480, 1994). SlSKP1 genes were highly expressed in primary parietal cells and primary sporogenous cells that develop into microspore mother cells in male flowers. Expression levels started to be reduced in the external stamens of the female flowers when stamen development was arrested at stage 7. Although microspore mother cells could not be developed in female flowers and SlSKP1 expression may be unnecessary in arrested stamens, SlSKP1 genes were still expressed in sporogenous cells of degenerated stamens at stage 8. Parietal cells stopped differentiating earlier than sporogenous cells in arrested stamens. These results suggest that not all types of cell are arrested simultaneously at a particular stage of stamen development during stamen suppression of S. latifolia.

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Isolation and characterization of two homeodomain leucine zipper genes from the dioecious plant Silene latifolia.

Cited by 8 publications

References 44 publications

Characterization of two SEPALLATA MADS-box genes from the dioecious plant Silene latifolia

Characterization of two SEPALLATA MADS-box genes from the dioecious plant Silene latifolia

Identification and characterization of homeobox genes in Eucalyptus

Expression of ASK1-like genes in arrested stamens of female Silene latifolia plants

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