Isolation and Characterization of Two New Deoxynivalenol-Degrading Strains, Bacillus sp. HN117 and Bacillus sp. N22

Li, Beibei; Duan, Jiaqi; Ren, Jie; Francis, Frédéric; Li, Guangyue

doi:10.3390/toxins14110781

Cited by 11 publications

(6 citation statements)

References 67 publications

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“…Microbial pure cultures and consortia capable of transforming DON have been isolated from various environmental sources, including soil [ 11 , 13 , 14 , 15 , 18 , 20 , 25 , 26 , 27 , 28 , 30 , 41 ], aquatic systems [ 16 , 17 ], animal intestines [ 23 , 24 , 42 ], and wheat [ 20 , 21 ]. This suggests that microorganisms able to metabolize DON are widespread in nature.…”

Section: Discussionmentioning

confidence: 99%

“…[ 17 , 18 ], Bacillus spp. [ 19 , 20 ], Marmoricola sp. MIM116 [ 21 ], Lactobacillus rhamnosus SHA113 [ 22 ], Eubacterium sp.…”

Section: Introductionmentioning

confidence: 99%

“…A6-243 [ 30 ]. Whether as pure or mixed cultures, these microbes transform DON primarily through oxidation or epimerization at the C3-OH group, hydroxylation at C16, de-epoxidation of the C12/C13 epoxy group, isomerization, and mineralization, thereby achieving biological detoxification [ 12 , 15 , 17 , 20 , 21 , 23 ]. Of all these pathways, the transformation mechanisms for the oxidation of DON’s C3-OH group and the subsequent epimerization are the most common and well-studied, especially in pure culture.…”

Section: Introductionmentioning

confidence: 99%

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Biotransformation of Deoxynivalenol by a Dual-Member Bacterial Consortium Isolated from Tenebrio molitor Larval Feces

Wang

Zhao

Zhang

et al. 2023

Toxins

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In this study, a dual-member bacterial consortium with the ability to oxidize deoxynivalenol (DON) to 3-keto-DON, designated SD, was first screened from the feces of Tenebrio molitor larvae. This consortium consisted of Pseudomonas sp. SD17-1 and Devosia sp. SD17-2, as determined by 16S rRNA-based phylogenetic analysis. A temperature of 30 °C, a pH of 8.0–9.0, and an initial inoculum concentration ratio of Devosia to Pseudomonas of 0.1 were optimal single-factor parameters for the DON oxidation activity of the bacterial consortium SD. Genome-based bioinformatics analysis revealed the presence of an intact PQQ biosynthesis operon (pqqFABCDEG) and four putative pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenase (ADH) genes in the genomes of Pseudomonas strain SD17-1 and Devosia strain SD17-2, respectively. Biochemical analyses further confirmed the PQQ-producing phenotype of Pseudomonas and the DON-oxidizing enzymatic activities of two of four PQQ-dependent ADHs in Devosia. The addition of PQQ-containing a cell-free fermentation supernatant from Pseudomonas activated DON-oxidizing activity of Devosia. In summary, as members of the bacterial consortium SD, Pseudomonas and Devosia play indispensable and complementary roles in SD’s oxidation of DON. Specifically, Pseudomonas is responsible for producing the necessary PQQ cofactor, whereas Devosia expresses the PQQ-dependent DON dehydrogenase, together facilitating the oxidation of DON.

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Section: Discussionmentioning

confidence: 99%

“…[ 17 , 18 ], Bacillus spp. [ 19 , 20 ], Marmoricola sp. MIM116 [ 21 ], Lactobacillus rhamnosus SHA113 [ 22 ], Eubacterium sp.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biotransformation of Deoxynivalenol by a Dual-Member Bacterial Consortium Isolated from Tenebrio molitor Larval Feces

Wang

Zhao

Zhang

et al. 2023

Toxins

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“…For example, mycelial fungi capable of synthesizing enzymes that hydrolyze some mycotoxins are producers of other mycotoxins. Bacterial cells of the genus Bacillus , according to Figure 2 B and Figure 3 F and the published data [ 106 ], can exhibit a high activity against ZEN, Afs, and DON, but these cells are producers of substances with pronounced antimicrobial activity against participants of methanogenic consortia. Moreover, this antimicrobial activity is enhanced when these compounds originated from Bacillus cells are combined with individual enzymes hydrolyzing mycotoxins [ 94 , 107 ].…”

Section: Expected Potential In Development Of Anaerobic Biocatalysts ...mentioning

confidence: 93%

Destruction of Mycotoxins in Poultry Waste under Anaerobic Conditions within Methanogenesis Catalyzed by Artificial Microbial Consortia

Ефременко

Senko

Maslova

et al. 2023

Toxins

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To reduce the toxicity of modern feeds polluted by mycotoxins, various sorbents are added to them when feeding animals. A part of the mycotoxins is excreted from the body of animals with these sorbents and remains in the manure. As a result, bulk animal wastes containing mixtures of mycotoxins are formed. It is known that it is partially possible to decrease the initial concentration of mycotoxins in the process of anaerobic digestion (AD) of contaminated methanogenic substrates. The aim of this review was to analyze the recent results in destruction of mycotoxins under the action of enzymes present in cells of anaerobic consortia catalyzing methanogenesis of wastes. The possible improvement of the functioning of the anaerobic artificial consortia during detoxification of mycotoxins in the bird droppings is discussed. Particular attention was paid to the possibility of effective functioning of microbial enzymes that catalyze the detoxification of mycotoxins, both at the stage of preparation of poultry manure for methanogenesis and directly in the anaerobic process itself. The sorbents with mycotoxins which appeared in the poultry wastes composed one of the topics of interest in this review. The preliminary alkaline treatment of poultry excreta before processing in AD was considered from the standpoint of effectively reducing the concentrations of mycotoxins in the waste.

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“…Li et al. (2022) separated and identified two DON‐degrading bacterial strains, Bacillus sp. HN117 and Bacillus sp.…”

Section: Don Biological Detoxification Methods and Nutritional Mitiga...mentioning

confidence: 99%

Global distribution, toxicity to humans and animals, biodegradation, and nutritional mitigation of deoxynivalenol: A review

Liu

Cai

et al. 2023

Comp Rev Food Sci Food Safe

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Deoxynivalenol (DON) is one of the main types of B trichothecenes, and it causes health‐related issues in humans and animals and imposes considerable challenges to food and feed safety globally each year. This review investigates the global hazards of DON, describes the occurrence of DON in food and feed in different countries, and systematically uncovers the mechanisms of the various toxic effects of DON. For DON pollution, many treatments have been reported on the degradation of DON, and each of the treatments has different degradation efficacies and degrades DON by a distinct mechanism. These treatments include physical, chemical, and biological methods and mitigation strategies. Biodegradation methods include microorganisms, enzymes, and biological antifungal agents, which are of great research significance in food processing because of their high efficiency, low environmental hazards, and drug resistance. And we also reviewed the mechanisms of biodegradation methods of DON, the adsorption and antagonism effects of microorganisms, and the different chemical transformation mechanisms of enzymes. Moreover, nutritional mitigation including common nutrients (amino acids, fatty acids, vitamins, and microelements) and plant extracts was discussed in this review, and the mitigation mechanism of DON toxicity was elaborated from the biochemical point of view. These findings help explore various approaches to achieve the best efficiency and applicability, overcome DON pollution worldwide, ensure the sustainability and safety of food processing, and explore potential therapeutic options with the ability to reduce the deleterious effects of DON in humans and animals.

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Isolation and Characterization of Two New Deoxynivalenol-Degrading Strains, Bacillus sp. HN117 and Bacillus sp. N22

Cited by 11 publications

References 67 publications

Biotransformation of Deoxynivalenol by a Dual-Member Bacterial Consortium Isolated from Tenebrio molitor Larval Feces

Biotransformation of Deoxynivalenol by a Dual-Member Bacterial Consortium Isolated from Tenebrio molitor Larval Feces

Destruction of Mycotoxins in Poultry Waste under Anaerobic Conditions within Methanogenesis Catalyzed by Artificial Microbial Consortia

Global distribution, toxicity to humans and animals, biodegradation, and nutritional mitigation of deoxynivalenol: A review

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