Isolation and Characterization of Uracil‐Degrading Clostridia from Soil

Mead, G. C.; Adams, B. W.; Hilton, M. G.; Lord, Peter G.

doi:10.1111/j.1365-2672.1979.tb00845.x

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…The strains of Escherichia coli and Bacillus coagulans were identified according to Cowan (1974). The clostridial isolates were characterized by the methods of Mead et al (1979) and, where possible, identified with the scheme of Smith (1970). The non-sporing anaerobes were characterized by the methods of Barnes & Impey (1974) and strains of Bacteroides hypermegas identified according to Cato & Barnes (1976) whilst B. vulgatus was identified as described by Cato & Johnson (1976).…”

Section: Origin and Composition Of Protective Bacterial Mixturementioning

confidence: 99%

Competitive exclusion of salmonellas from the chick caecum using a defined mixture of bacterial isolates from the caecal microflora of an adult bird

Impey

Mead

George

1982

J. Hyg.

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SummaryColonization of the caeca of newly hatched chicks by Salmonella typhimurium was prevented by oral administration of a mixture of cultures comprising 48 different bacterial strains originating from an adult bird known to be free from salmonellas. The treatment conferred protection to the same degree as that obtained previously with a suspension of adult caecal contents or an undefined anaerobic culture from the same source and was demonstrated in four separate laboratory trials.Examination of the caecal microflora of chicks one day after being given the protective treatment showed that the presence of high levels of lactobacilli and Bacteroides spp. which are not found usually at two days of age in chicks produced under commercial conditions was indicative of the successful establishment of an adult-type microflora.Although the usual method of administering the protective organisms was to dose the chicks directly into the crop, it was also found possible to incorporate the organisms in the drinking water given to the birds at dilutions up to one in five, the maximum tested.When chicks were given the bacterial mixture via the crop and fed on a diet containing 10 mg kg−1 nitrovin and 100 mg kg−1 monensin, the bacteroides failed to establish in the caeca and the birds were not protected against salmonella colonization. However, when the bacterial cultures were incorporated in the drinking water and the chicks given the same feed, normal protection was obtained; possible reasons for these observations are discussed.

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Section: Origin and Composition Of Protective Bacterial Mixturementioning

confidence: 99%

Competitive exclusion of salmonellas from the chick caecum using a defined mixture of bacterial isolates from the caecal microflora of an adult bird

Impey

Mead

George

1982

J. Hyg.

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“…They are, however, poor growth substrates [289,290]. Among the proteolytic species, C. sporogenes and some types of C. botulinum were found to deaminate cytosine and to reduce uracil and thymine to the dihydro forms, but further metabolism was not observed [291].…”

Section: Metabolism Of Heterocyclic Compoundsmentioning

confidence: 99%

General Biology and Physiology

Mitchell

2001

Clostridia

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“…Clostridium uracilicum metabolized uracil to DHU, Ncarbamyl-fl-alanine (fl-ureidopropionic acid) and fl-alanine, carbon dioxide and ammonia (Campbell 1957). Clostridium olycolicum hydrolyses uracil to fl-alanine while neither D H U nor N-carbamyl-fl-alanine were detected (Mead et al 1979). C. sporooenes, C. botulinum type A and proteolytic strains of C. botulinum type B metabolized uracil to D H U without any further breakdown and without any stimulation of growth by uracil (Hilton et al 1975).…”

Section: Introductionmentioning

confidence: 99%

d-Hydantoinase from anaerobic microorganisms

Morin¹,

Touzel²,

Lafond

et al. 1991

Appl Microbiol Biotechnol

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Of 373 anaerobic microbial isolates screened for the enzymatic conversion of dihydrouracii to N-carbamyl-fl-alanine, several strains of Clostridium spp., C. glycolicum, C. subterminale and Peptococcus anaerobius were positive. These Clostridium and Peptococcus strains produced also N-carbamyl-D-amino acids from the respective 5'-monosubstituted hydantoins. The Dhydantoinase activity from whole cell suspensions of P. anaerobius strain C R D A 303 was characterized with regard to pH and temperature stability and activity by using dihydrouracil (DHU) and isopropylhydantoin (IPH) as substrates. The D-hydantoinase from P. anaerobius was optimal at 60°C and at pH 6.5-9.5 for the substrate DHU. It was stable up to 55°C and at pH 5.0-9.5 and could be stored at 4°C under an aerobic atmosphere for at least 14 days.

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Isolation and Characterization of Uracil‐Degrading Clostridia from Soil

Cited by 7 publications

References 9 publications

Competitive exclusion of salmonellas from the chick caecum using a defined mixture of bacterial isolates from the caecal microflora of an adult bird

Competitive exclusion of salmonellas from the chick caecum using a defined mixture of bacterial isolates from the caecal microflora of an adult bird

General Biology and Physiology

d-Hydantoinase from anaerobic microorganisms

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