Isolation and characterization of Vibrio harveyi as a major pathogen associated with mass mortalities of ark clam, Scapharca broughtonii, in summer

Wei, Zhixin; Xin, Lusheng; Zhang, Weiwei; Bai, Changming; Chongming, Wang; Li, Chenghua

doi:10.1016/j.aquaculture.2019.734248

Cited by 16 publications

(16 citation statements)

References 34 publications

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“…Another pathogenic agent, belonging to Vibrionaceae family, causing mortalities in a wide variety of aquatic animals is V. harveyi , although it has been shown to belong to the microbiome of most of the infected hosts [ 62 ]. V. harveyi strains have been detected in many hosts such as crabs, teleosts, crustaceans, and bivalve mollusks, causing immune and physiological depression and mortalities [ 63 , 64 , 65 , 66 , 67 , 68 ]. V. mediterranei is considered to be an emerging pathogen, being abundant in aquatic environments, infecting hosts such as corals and seaweeds and causing mortalities synergistically with other pathogens [ 69 , 70 ].…”

Section: Discussionmentioning

confidence: 99%

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

Lattos

Bitchava²,

Giantsis

et al. 2021

Microorganisms

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Pinna nobilis populations, constituting the largest bivalve mollusk endemic to the Mediterranean, is characterized as critically endangered, threatened by extinction. Among the various factors proposed as etiological agents are the Haplosporidium pinnae and Mycobacterium sp. parasites. Nevertheless, devastation of the fan mussel populations is still far from clear. The current work is undertaken under a broader study aiming to evaluate the health status of Pinna nobilis population in Aegean Sea, after the mass mortalities that occurred in 2019. A significant objective was also (a) the investigation of the etiological agents of small-scale winter mortalities in the remaining populations after the devastating results of Haplosporidium pinnae and Mycobacterium sp. infections, as well as (b) the examination of the susceptibility of the identified bacterial strains in antibiotics for future laboratory experiments. Microbiological assays were used in order to detect the presence of potential bacterial pathogens in moribund animals in combination with molecular tools for their identification. Our results provide evidence that Vibrio bacterial species are directly implicated in the winter mortalities, particularly in cases where the haplosporidian parasite was absent. Additionally, this is the first report of Vibrio mediterranei and V. splendidus hosted by any bivalve on the Greek coastline.

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Section: Discussionmentioning

confidence: 99%

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

Lattos

Bitchava²,

Giantsis

et al. 2021

Microorganisms

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“…The bacterium is an important pathogen of farmed aquatic organisms and it can infect a wide range of marine fish and invertebrates (Austin and Zhang 2006; Wei et al . 2019). Diseases associated with V. harveyi (described as vibriosis) are common in maricultured organisms worldwide (Toranzo et al .…”

Section: Introductionmentioning

confidence: 99%

“…2019; Wei et al . 2019). Vibrio harveyi seems to have a greater impact on farmed marine animal species in China as mariculture increases.…”

Section: Introductionmentioning

confidence: 99%

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Vibrio harveyi isolated from marine aquaculture species in eastern China and virulence to the large yellow croaker ( Larimichthys crocea )

Yang

Zhang

et al. 2021

J Appl Microbiol

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Aims Diseases of maricultured species caused by Vibrio harveyi are increasing in China and other regions. This study examined the genetic diversity, antimicrobial susceptibility, plasmid profiles and virulence potential of the V. harveyi isolated from marine organisms farmed in two provinces in eastern China between 2014 and 2019. Methods and Results A total of 54 V. harveyi were obtained from seven marine species. Enterobacterial repetitive intergenic consensus (ERIC)‐PCR fingerprinting revealed substantial genetic heterogeneity among the V. harveyi isolates. There was no significant correlation between ERIC‐PCR genotypes and host origins or fish farms. All the isolates were resistant to amoxicillin and ampicillin, and 79·6% to kanamycin. We found that 61·1% of the V. harveyi isolates had plasmid(s) and there were 14 different plasmid profiles. Most isolates from fish hosts (76·5%) contained plasmids; however, 75% of isolates from nonfish hosts lacked plasmids. Experimental infection results showed that isolates with plasmid(s) were more virulent to large yellow croaker than isolates lacking plasmids (P < 0·05). Conclusions This study confirmed that V. harveyi isolates obtained from animals farmed in the coastal region of east China were genetically diverse. Our results suggest that the virulence of various V. harveyi strains to fish is associated with the plasmids they carry. Significance and Impact of the Study More than 50% of the V. harveyi isolates carried one to 11 plasmids. The plasmid‐borne traits of V. harveyi strains might be important for host adaptation and virulence, but they were not associated with susceptibility to the tested antibiotics.

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“…V. harveyi is one of the important pathogens of aquatic animals, which poses a serious threat to marine aquaculture (Alvarez, 1998;Wei et al, 2019). Our earlier work revealed that V. harveyi could enter into viable but nonculturable (VBNC) state and resuscitated V. harveyi cells were found to retain their pathogenicity (Sun et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Expression and protease characterization of a conserved protein YgjD in Vibrio harveyi

Zhang

Chen

Wang

et al. 2020

PeerJ

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The glycopeptidase GCP and its homologue proteins are conserved and essential for survival of bacteria. The ygjD gene (Glycopeptidase homologue) was cloned from Vibrio harveyi strain SF-1. The gene consisted of 1,017 bp, which encodes a 338 amino acid polypeptide. The nucleotide sequence similarity of the ygjD gene with that of V. harveyi FDAARGOS 107 was 95%. The ygjD gene also showed similarities of 68%, 67% and 50% with those of Salmonella enterica, Escherichia coli and Bacillus cereus. The ygjD gene was expressed in E. coli BL21 (DE3) and the recombinant YgjD was purified by Ni2+ affinity chromatography column. The purified YgjD showed a specific 37 kDa band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and exhibited protease activities of 59,000 units/mg, 53,700 units/mg and 8,100 units/mg, respectively, on N-Acetyl-L-tyrosine ethyl ester monohydrate (ATEE), N-Benzoyl-L-tyrosine ethyl ester (BTEE) and N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride (BAPNA) substrates. When the conserved amino acids of His111, Glu113 and His115 in the YgjD were replaced with alanine, respectively, the protease activities of the mutants were partly decreased. The two conserved His111 and His115 of YgjD were mutated and the protein lost the protease activity, which implied that the two amino acid played very important roles in maintaining its protease activity. The addition of the purified YgjD to the culture medium of V. harveyi strain SF-1 can effectively promote the bacteria growth. These results indicated that the protease activities may be involved in the survival of bacteria.

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Isolation and characterization of Vibrio harveyi as a major pathogen associated with mass mortalities of ark clam, Scapharca broughtonii, in summer

Cited by 16 publications

References 34 publications

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

Vibrio harveyi isolated from marine aquaculture species in eastern China and virulence to the large yellow croaker ( Larimichthys crocea )

Expression and protease characterization of a conserved protein YgjD in Vibrio harveyi

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