Isolation and characterization of yeast mitochondrial mutants defective in spore germination

Hartig, Andreas; Schroeder, Renée; Mucke, Eva; Breitenbach, Michael

doi:10.1007/bf00376783

Cited by 19 publications

(6 citation statements)

References 20 publications

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“…In sporulating cells, mitochondria undergo extensive morphological and functional differentiation and are actively distributed to the four ascospores (Brewer and Fangman, 1980). The mitochondria of mature spores are devoid of cytochromes and morphologically resemble the promitochondria of anaerobic cells, reflecting the metabolically resting state of mature spores (Brewer and Fangman, 1980; Hartig et al ., 1981).…”

Section: Resultsmentioning

confidence: 99%

Systematic analysis of sporulation phenotypes in 624 non‐lethal homozygous deletion strains of Saccharomyces cerevisiae

Briza

Bogengruber

Thür

et al. 2002

Yeast

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A new high throughput mutant screening procedure for the detection of sporulation mutants was developed and used to analyse a set of 624 non-lethal homozygous deletion mutants created in the European joint research program EUROFAN. The screening procedure involved determination of LL- and DL-dityrosine, sporulation-specific compounds, which were shown to be robust markers of the extent and arrest stage of sporulation mutants. Secondary screens consisted of light microscopy to detect mature and immature spores and DAPI staining to monitor the progress of meiotic nuclear divisions. We discovered new phenotypic classes of mutants defective in spore wall synthesis that were not discovered by previous screens for sporulation mutants. The genes corresponding to the sporulation mutants fell in several functional classes, some of which were previously unknown to be involved in spore formation. Peroxisomes seem to play a role in spore wall synthesis. Mitochondria play a role in sporulation that is not simply restricted to supply of ATP from respiratory metabolism. The deletion mutants included in the set were functionally unknown at the start of EUROFAN; however, within the last few years the importance to sporulation of some of them was also reported by other authors. Taken together, about 8% of all single gene deletion mutants of non-essential genes of Saccharomyces cerevisiae seem to display a clear and reproducible sporulation phenotype.

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Section: Resultsmentioning

confidence: 99%

Systematic analysis of sporulation phenotypes in 624 non‐lethal homozygous deletion strains of Saccharomyces cerevisiae

Briza

Bogengruber

Thür

et al. 2002

Yeast

Self Cite

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“…Expression of Ssc1p, a mitochondrial hsp70 protein, from the uninduced GAL7 promoter supports mitotic growth but not the outgrowth of spores (33). Also, mitochondrial mutants that inhibit only the outgrowth of mutant spores have been identified (22). Finally, spore wall mutations can sensitize spores to damage by procedures used for genetic analysis and thereby exert specific inhibition of spore germination (1,3,53).…”

Section: Discussionmentioning

confidence: 99%

Prenylated Isoforms of Yeast Casein Kinase I, Including the Novel Yck3p, Suppress the gcs1 Blockage of Cell Proliferation from Stationary Phase

Wang

Hoekstra

Demaggio

et al. 1996

Molecular and Cellular Biology

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The GCS1 gene of the budding yeast Saccharomyces cerevisiae mediate the resumption of cell proliferation from the starved, stationary-phase state. Here we identify yeast genes that, in increased dosages, overcome the growth defect of gcs1 delta mutant cells. Among these are YCK1 (CK12) and YCK2 (CKI1), encoding membrane-associated casein kinase I, and YCK3, encoding a novel casein kinase I isoform. Some Yck3p gene product was found associated with the plasma membrane, like Yck1p and Yck2p, but most confractionated with the nucleus, like another yeast casein kinase I isoform, Hrr25p. Genetic studies showed that YCK3 and HRR25 constitute an essential gene family and that Yck3p can weakly substitute for Yck1p-Yck2p. For gcs1 delta suppression, both a protein kinase domain and a C-terminal prenylation motif were shown to be necessary. An impairment in endocytosis was found for gcs1 delta mutant cells, which was alleviated by an increased YCK2 gene dosage. The ability of an increased casein kinase I gene dosage to suppress the effects caused by the absence of Gcs1p suggests that Gcs1p and Yck1p-Yck2p affect parallel pathways.

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Section: Genes Involved In Autophagocytosis Vacuole Assembly or Vacumentioning

confidence: 99%

Systematic analysis of sporulation phenotypes in 624 non-lethal homozygous deletion strains of Saccharomyces cerevisiae

Briza¹,

Bogengruber²,

Thür³

et al. 2002

Yeast

Self Cite

View full text Add to dashboard Cite

A new high throughput mutant screening procedure for the detection of sporulation mutants was developed and used to analyse a set of 624 non-lethal homozygous deletion mutants created in the European joint research program EUROFAN. The screening procedure involved determination of LL-and DL-dityrosine, sporulation-specific compounds, which were shown to be robust markers of the extent and arrest stage of sporulation mutants. Secondary screens consisted of light microscopy to detect mature and immature spores and DAPI staining to monitor the progress of meiotic nuclear divisions. We discovered new phenotypic classes of mutants defective in spore wall synthesis that were not discovered by previous screens for sporulation mutants. The genes corresponding to the sporulation mutants fell in several functional classes, some of which were previously unknown to be involved in spore formation. Peroxisomes seem to play a role in spore wall synthesis. Mitochondria play a role in sporulation that is not simply restricted to supply of ATP from respiratory metabolism. The deletion mutants included in the set were functionally unknown at the start of EUROFAN; however, within the last few years the importance to sporulation of some of them was also reported by other authors. Taken together, about 8% of all single gene deletion mutants of non-essential genes of Saccharomyces cerevisiae seem to display a clear and reproducible sporulation phenotype.

show abstract

Isolation and characterization of yeast mitochondrial mutants defective in spore germination

Cited by 19 publications

References 20 publications

Systematic analysis of sporulation phenotypes in 624 non‐lethal homozygous deletion strains of Saccharomyces cerevisiae

Systematic analysis of sporulation phenotypes in 624 non‐lethal homozygous deletion strains of Saccharomyces cerevisiae

Prenylated Isoforms of Yeast Casein Kinase I, Including the Novel Yck3p, Suppress the gcs1 Blockage of Cell Proliferation from Stationary Phase

Systematic analysis of sporulation phenotypes in 624 non-lethal homozygous deletion strains of Saccharomyces cerevisiae

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