Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

Mancini, Maria Emanuela; Beverelli, Matteo; Donatiello, Adelia; Didonna, Antonella; D’Attoli, Luigi; Faleo, Simona; Occhiochiuso, Gilda; Galante, Domenico; Rondinone, Valeria; Sambro, Laura Del; Bianco, Angela; Miccolupo, Angela; Goffredo, Elisa

doi:10.1371/journal.pone.0268706

Cited by 8 publications

(4 citation statements)

References 60 publications

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“…Under these considerations, the relationship between foods and human disease is still strongly debated as the "nonpathogenic" biotype 1A is frequently isolated from foods (Le Guern et al, 2016;Guiller et al, 2021). Even in the present research all the isolated strains were negative for the detection of ail gene as reported by other authors (Mancini et al, 2022). Furthermore, all the Y. enterocolitica strains isolated from diseased people in Umbria region were positive for ail gene detection (Primavilla et al, 2017) even in the same period of observation (data not reported).…”

Section: Articlesupporting

confidence: 58%

“…2006) but Y. enterocolitica is reported also in other animal species (ZhiChao et al, 2019;Arden et al, 2022). In foods Y. enterocolitica isolation is reported from meat, milk and dairy products and fresh vegetables (Bonardi et al, 2018;Fois et al, 2018;Espenhain et al, 2019;Piras et al, 2021;Mancini et al, 2022). The microorganism can resist in food under different environmental situations, even in not culturable form, and can grow under refrigeration conditions (Fredriksson-Ahomaa, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Retrospective study on the prevalence of <em>Yersinia enterocolitica</em> in food collected in Umbria region (central Italy)

Primavilla¹,

Farneti²,

Roila³

et al. 2023

Ital J Food Safety

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Yersinia enterocolitica represents one of the main foodborne pathogens in Europe and the evaluation of possible sources of contamination and its prevalence in food is of considerable interest for risk analysis approach. The results of the search for Yersinia enterocolitica in food samples taken in Umbria region (central Italy) were evaluated during the years 2015-2018. Different types of foods were considered, both ready-to-eat (meat products, dairy products, and raw vegetables) and meat preparations to be eaten after cooking. Samples were assayed by molecular screening for the species indicator gene ompF. Screening positives were subjected to isolation and characterization by searching for specific virulence marker genes, including the ail gene responsible for invasiveness and the ystB gene for the production of enterotoxin. The total prevalence of positive samples for Yersinia enterocolitica was 16.86% with a higher percentage of positive samples in meat preparations (19.35%), followed by ready-to-eat vegetables (11.76%). Poultry meat samples had a higher prevalence than pork and beef samples. Neither positive samples were found in meat products and dairy, nor seasonality in positivity was observed. All isolated strains of Yersinia enterocolitica were biotype 1A, with absence of the ail virulence gene but presence of ystB gene. Since the strains isolated from human patients appear to be primarily biotypes that possess the ail marker, future investigations would be needed regarding the real role of biotype 1A in human disease. In this context, attention should certainly be paid to ready-to-eat vegetables and to careful cooking of meat preparations.

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Section: Articlesupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Retrospective study on the prevalence of <em>Yersinia enterocolitica</em> in food collected in Umbria region (central Italy)

Primavilla¹,

Farneti²,

Roila³

et al. 2023

Ital J Food Safety

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“…All the presumptive Vibrio spp. isolates were identified at species level based on the API ® ID 20E and 20NE systems (BioMérieux, Nürtingen, Germany), the halotolerance test with various concentrations of NaCl (0, 6 and 10%), and the MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) procedure by the direct transfer method, as previously described [ 25 ]. Moreover, the isolates recognised as V. parahaemolyticus , V. cholerae , and V. vulnificus were confirmed by the conventional PCR method described in Annex C to ISO 21872–1:2017, which considers, also, the detection of V. parahaemolyticus virulence genes ( tdh and trh ).…”

Section: Methodsmentioning

confidence: 99%

Systematic Survey of Vibrio spp. and Salmonella spp. in Bivalve Shellfish in Apulia Region (Italy): Prevalence and Antimicrobial Resistance

et al. 2023

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The emergence of antimicrobial resistance (AMR) is increasingly common across the globe and aquatic ecosystems could be considered a reservoir of antibiotic-resistant bacteria. This study aimed to determine prevalence and antibiotic susceptibility of the potential pathogenic bacteria Salmonella spp. and Vibrio spp. in bivalve molluscs intended for human consumption, collected over a period of 19 months along the northern coast of Apulia region. The AMR profile was also determined in non-pathogenic Vibrio species, common natural inhabitants of seawater and a useful indicator for the surveillance of AMR in the environment. The current study presents data on the AMR of 5 Salmonella and 126 Vibrio isolates by broth microdilution MIC. Multidrug resistance (MDR) was observed in one S. Typhimurium strain towards sulfamethoxazole, trimethoprim, tetracycline, gentamicin, and ampicillin and in 41.3% of the Vibrio strains, mostly towards sulphonamides, penicillin, and cephems. All Vibrio isolates were sensitive to azithromycin, chloramphenicol, tetracycline, amoxicillin/clavulanic acid, gentamicin, streptomycin, amikacin, and levofloxacin. The AMR phenomenon in the investigated area is not highly worrying but not entirely negligible; therefore, in-depth continuous monitoring is suggested. Results concerning the antibiotic agents without available specific clinical breakpoints could be useful to upgrade the MIC distribution for Vibrio spp. but, also, the establishment of interpretative criteria for environmental species is necessary to obtain a more complete view of this issue.

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“…Th e genus Yersinia spp. belongs to the Enterobacteriaceae family and includes 18 species, of which 3 species, i.e., Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis, cause infection in humans and animals [2]. Y. enterocolitica was categorized into two subspecies based on 16s rRNA gene type: Y. enterocolitica subsp.…”

mentioning

confidence: 99%

Comparative Genomics, Phylogenetic and Functional Analysis of Yersinia enterocolitica, a Gastrointestinal Pathogen, with Other Soil-Borne Bacteria Causing Diseases

Al-Rawe,

Al-Jomaily,

Yousif

et al. 2023

Mikrobiol. Z.

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Yersinia enterocolitica is a harmful bacterium transmitted through contaminated food, causing gastrointestinal illness and lymph node inflammation. The rise of drug-resistant strains of Y. enterocolitica poses a serious public health threat, necessitating research on its ecology, related species, and unique genes linked to virulence and antibiotic resistance. This study identified eight microorganisms similar to Y. enterocolitica and conducted a pan-genomic analysis, revealing specific genes exclusive to Y. enterocolitica. Enrichment analysis of these genes unveiled their involvement in antibiotic synthesis pathways, such as siderophore production, osmoregulated periplasmic glucan activation, and antibiotic resistance. These pathways, including biofilm formation and increased antibiotic tolerance, are vital for Yersinia’s virulence. Furthermore, specific genes related to glutamate metabolism, nitrogen regulation, motility, purine, and pyrimidine synthesis may contribute to Y. enterocolitica’s pathogenicity, growth, and virulence factor production. Phylogenetic analysis demonstrated the evolutionary relationship between Y. enterocolitica and similar species like Escherichia coli, Campylobacter jejuni, and Salmonella enterica, stressing the need to monitor Y. enterocolitica in slaughterhouses due to animal carriers. The study’s findings shed light on the ecological factors and genetic mechanisms driving Y. enterocolitica’s pathogenicity and antibiotic resistance. Targeting genes involved in purine and pyrimidine synthesis, such as ushA, cpdB, and deoB, could be potential strategies for controlling pathogenicity and antimicrobial resistance. Understanding the relationships and genetic interactions between Y. enterocolitica and related microorganisms is crucial for developing effective surveillance and management approaches in the future.

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Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

Cited by 8 publications

References 60 publications

Retrospective study on the prevalence of <em>Yersinia enterocolitica</em> in food collected in Umbria region (central Italy)

Retrospective study on the prevalence of <em>Yersinia enterocolitica</em> in food collected in Umbria region (central Italy)

Systematic Survey of Vibrio spp. and Salmonella spp. in Bivalve Shellfish in Apulia Region (Italy): Prevalence and Antimicrobial Resistance

Comparative Genomics, Phylogenetic and Functional Analysis of Yersinia enterocolitica, a Gastrointestinal Pathogen, with Other Soil-Borne Bacteria Causing Diseases

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