Isolation and characterization of γδ T lymphocyte cell lines from Sinclair swine peripheral blood

Grimm, David R.; Richerson, Joan T.; Theiss, Patty; LeGrand, Roy D.; Misfeldt, Michael L.

doi:10.1016/0165-2427(93)90110-p

Cited by 15 publications

(6 citation statements)

References 41 publications

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“…With regard to the specificity of our mAbs, a peculiar form of the CD3-TcR complex could possibly be recognized by mAb 1E7, since this could co-precipitate the IP 90-like TcR chaperon, recognized by mAb AF8. The M, of the proteins detected by mAb 1E7 does not fit into previous data about bovine TcR (MACKAY and HEIN, 1989;MORRISON et al, 1989); however, a similar pattern of reactivity has recently been shown for a yS T cell line of swine, whereby components of 40 and 75 kDa apparent molecular mass were shown to be expressed together by Western blotting with a TcR 6 antiserum (GRIMM et al, 1993).…”

Section: Discussioncontrasting

confidence: 57%

Characterization of a Distinct Subpopulation of Bovine γδ T cells

Amadori

Archetti

Verardi

et al. 1995

Journal of Veterinary Medicine, Series B

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Summary A population of mononuclear cytotoxic cells from peripheral blood leucocytes of cattle showed no usual markers of B and T lymphocytes. However, it could be allocated to a previously unreported γδ T cell compartment. This assumption was suggested by: 1. The surface expression of CD3; 2. PCR amplification of the Cδ TcR gene from cDNA; and 3. The detection of peripheral blood precursors expressing the workshop cluster (WC) 1 marker of bovine γδ T cells. These cells are recognized by murine monoclonal antibodies (mAbs) 5D4, 1E7, 6F9 and 8D7, raised in the authors' laboratory. The above mAbs also identify distinct groups of cells in thymus, spleen, lymph nodes and about 1 % of uncultured PBL. The most diffuse infiltration of such cells was shown in the small intestine, as both intraepithelial and lamina propria lymphocytes. Mucosal homing activity was confirmed by immunoperoxidase staining on tongue and pharynx sections of healthy cattle.

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Section: Discussioncontrasting

confidence: 57%

Characterization of a Distinct Subpopulation of Bovine γδ T cells

Amadori

Archetti

Verardi

et al. 1995

Journal of Veterinary Medicine, Series B

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“…The exact role of Vγ1 and Vδ1 TCR + cells in tumour immunology has still to be defined; however, γ/δ TCR + cells may be stimulated to secrete lymphokines and may act as activated killer cells and exert some cytolytic activity on several tumours 1. Extending earlier porcine7 and human6 28 reports on the existence of these cells in melanomas and the occurrence of spontaneous regression of melanomas,11 patient survival was analysed with respect to the infiltration by these cells. Interestingly, a highly significant positive correlation for survival could be defined.…”

Section: Discussionmentioning

confidence: 99%

alpha /beta - and gamma /delta TCR+ lymphocyte infiltration in necrotising choroidal melanomas

Bialasiewicz

Richard

1999

British Journal of Ophthalmology

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T cells can play a central role in the local immune response to cancer with tumour specific T lymphocyte reactivity provided by the TCR variable genes V /V chain heterodimer or the V or V chains. The identification and functional characterisation of MHC class I restricted antigen specific TCR expressing tumour infiltrating lymphocytes (TIL) has been the subject of intense research in the recent years, since tumour reactivity by these genes may provide novel applications for immunotherapy including the redirection of TIL specificity, and bone marrow stem cell therapy.

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“…Porcine CD2 + CD4 7 CD8 lo gd T cells are capable of cytotoxic activity (Grimm et al, 1993;Pauly et al, 1996;Yang and Parkhouse, 1997;De Bruin et al, 2000). This activity is antigen-non-specific and MHC-nonrestricted Pauly et al, 1996).…”

Section: Porcine G D T Cellsmentioning

confidence: 99%

Biology of porcine T lymphocytes

Charerntantanakul

Roth

2006

Anim. Health. Res. Rev.

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The present review concentrates on the biological aspects of porcine T lymphocytes. Their ontogeny, subpopulations, localization and trafficking, and responses to pathogens are reviewed. The development of porcine T cells begins in the liver during the first trimester of fetal life and continues in the thymus from the second trimester until after birth. Porcine T cells are divided into two lineages, based on their possession of the alphabeta or gammadelta T-cell receptor. Porcine alphabeta T cells recognize antigens in a major histocompatibility complex (MHC)-restricted manner, whereas the gammadelta T cells recognize antigens in a MHC non-restricted fashion. The CD4+CD8- and CD4+CD8lo T cell subsets of alphabeta T cells recognize antigens presented in MHC class II molecules, while the CD4-CD8+ T cell subset recognizes antigens presented in MHC class I molecules. Porcine alphabeta T cells localize mainly in lymphoid tissues, whereas gammadelta T cells predominate in the blood and intestinal epithelium of pigs. Porcine CD8+ alphabeta T cells are a prominent T-cell subset during antiviral responses, while porcine CD4+ alphabeta T cell responses predominantly occur in bacterial and parasitic infections. Porcine gammadelta T cell responses have been reported in only a few infections. Porcine T cell responses are suppressed by some viruses and bacteria. The mechanisms of T cell suppression are not entirely known but reportedly include the killing of T cells, the inhibition of T cell activation and proliferation, the inhibition of antiviral cytokine production, and the induction of immunosuppressive cytokines.

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Isolation and characterization of γδ T lymphocyte cell lines from Sinclair swine peripheral blood

Cited by 15 publications

References 41 publications

Characterization of a Distinct Subpopulation of Bovine γδ T cells

Characterization of a Distinct Subpopulation of Bovine γδ T cells

alpha /beta - and gamma /delta TCR+ lymphocyte infiltration in necrotising choroidal melanomas

Biology of porcine T lymphocytes

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