Isolation and chromosomal localization of ecdysterone-responsive genes in a Drosophila cell line

Couderc, J.L.; Becker, Jacqueline; Sobrier, Marie-Laure; Dastugue, B.; Best‐Belpomme, Martin; Lepesant, Jean‐Antoine; Pardue, Mary Lou

doi:10.1007/bf00331250

Cited by 14 publications

(9 citation statements)

References 26 publications

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“…It is remarkable that, for all the examples of 'positive' results, satisfactory explanations can be given by simple interpretations in terms of individual polymorphisms or selection, instead of specific induction by the applied stress. Correlation is shown in particular situations, where the element itself is regulated by a heat-shock promoter or by a hormone, as for the 1731 element of D. melanogaster (Couderc et al, 1984). In Dictyostelium discoMeum, a mobile repeated sequence was activated by heat shock at the same time as the heat-shock gene where it was inserted, but the stress was not demonstrated to be responsible for this activation (Rosen, Sivertsen & Firtel, 1983).…”

Section: Experiments With Environmental Factorsmentioning

confidence: 93%

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Arnault

Dufournel

1994

Genetica

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The action of stresses on the genome can be considered as responses of cells or organisms to external aggressions. Stress factors are of environmental origin (climatic or trophic) or of genomic nature (introduction of foreign genetic material, for example). In both cases, important perturbations can occur and modify hereditary potentialities, creating new combinations compatible with survival; such a situation may increase the variability of the genome, and allow evolutive processes to take place. The behavior of transposable elements under stress conditions is thus of particular interest, since these sequences are sources of mutations and therefore of genetic variability; they may play an important role in population adaptation. The survey of the available experimental results suggest that, although some examples of mutations and transposable elements movements induced by external factors are clearly described, environmental injuries or introduction of foreign material into a genome are not systematically followed by drastic genomic changes.

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Section: Experiments With Environmental Factorsmentioning

confidence: 93%

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Arnault

Dufournel

1994

Genetica

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“…XO7656) is a D. melanogaster copia-like element, a LTR-retrotransposon (Hemivirus, Pseudoviridae), cloned during analysis of the action of ecdysone on Kc cells (Couderc et al, 1984). It is represented in the Drosophila genome as about 10-12 copies with 2 types of structure (Fig.…”

Section: Introductionmentioning

confidence: 99%

Amplification of the 1731 LTR retrotransposon in Drosophila melanogaster cultured cells: Origin of neocopies and impact on the genome

Maisonhaute

Ogereau

Hua-Van

et al. 2007

Gene

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“…At physiological concentrations, ecdysterone induces a wide variety of cellular responses such as morphological changes (2), arrest of division (3), protein variations (4) and induction of acetylcholinesterase (5,6), p-galactosidase (7) and catalase activities (8) (for a review, see 9). This capacity to respond to a steroid hormone prompted the use of the polyA+ transcripts from these cells for the isolation of DNA sequences whose expression is either increased ("inducible genes") or decreased ("repressed genes") by the steroid (10). The genes have the characteristics of repeated transposable elements (10).…”

Section: Introductionmentioning

confidence: 99%

“…This capacity to respond to a steroid hormone prompted the use of the polyA+ transcripts from these cells for the isolation of DNA sequences whose expression is either increased ("inducible genes") or decreased ("repressed genes") by the steroid (10). The genes have the characteristics of repeated transposable elements (10).…”

Section: Introductionmentioning

confidence: 99%

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1731, A New Retrotransposon with Hormone Modulated Expression

Peronnet¹,

Becker²,

Lescault³

et al. 1988

Invertebrate and Fish Tissue Culture

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We report here the characterisation of 1731, a new copia-like element of Drosophila melanogaster. 1731 was first isolated in a screening for ecdysterone modulated genes. This element is about 4.6 Kb long and is flanked by two long terminal repeats (LTRs) 336 base pairs in length. The whole 1731 element is transcribed into polyA+ RNAs, and these transcripts decrease rapidly upon hormonal treatment. 1731 is moderately repeated in the fly genome and slightly amplified in Kc cells where extrachromosomal circular forms are found. The LTRs were sequenced in one cloned copy of 1731 and show a structural organisation similar to that of several other copia-like elements and retroviral proviruses. Small nucleotide stretches, similar to those found in Mouse Mammary Tumor Virus LTRs and known to be important in its regulation by a steroid hormone, occur in 1731 LTRs. hybridizations of these hormone modulated sequences on Drosophila salivary glands giant chromosomes showed that inducible genes appear to be single copy while the repressed

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Isolation and chromosomal localization of ecdysterone-responsive genes in a Drosophila cell line

Cited by 14 publications

References 26 publications

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Amplification of the 1731 LTR retrotransposon in Drosophila melanogaster cultured cells: Origin of neocopies and impact on the genome

1731, A New Retrotransposon with Hormone Modulated Expression

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