Isolation and Cultivation of Some Pathogen Fungi from Apple and Grapevines Grown in Arges County

Ungureanu, Camelia; Călinescu, Mirela; Ferdeş, Mariana; Soare, Liliana Cristina; Vizitiu, Diana Elena; Fierăscu, Irina; Fierăscu, Radu Claudiu; Răileanu, Silviu

doi:10.37358/rc.19.11.7671

Cited by 7 publications

(4 citation statements)

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“…In terms of food safety, species of the Fusarium, Aspergillus, and Penicillium genera are considered the most significant because they produce the great majority of known mycotoxins [49]; at the same time, Venturia inaequalis and Podosphaera leucotricha represent the most pathogenic microorganisms that affect apple crops. Many important information about the plant pathology aspects are described in the literature [50].…”

Section: In Vitro Antimicrobial Activitymentioning

confidence: 99%

“…The sensitivity of the microorganisms to the tested sample was established by the diameter of the inhibition zones according to Ponce et al [53] as not sensitive (diameter under 8 mm), sensitive (diameter from 9 to 14 mm), very sensitive (diameter from 15 to 19 mm), and extremely sensitive (diameter above 20 mm). The biological strains used for this investigation were E. coli, S. aureus, P. aeruginosa, S. typhimurium, C. albicans, F. oxysporum, P. hirsutum, A. niger and V. inaequalis, and P. leucotricha isolated from fruit samples provided by the Research Institute for Fruit Growing Pitesti-Mărăcineni [50]. Each experiment was carried out in triplicate with three separate assay runs, using as negative control the solvent of the extracts (water: ethanol or ethanol) and as positive control commercial antimicrobials.…”

Section: In Vitro Antimicrobial Activitymentioning

confidence: 99%

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In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

Ungureanu

Fierăscu

et al. 2021

Materials

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The aim of the current paper is the development of phytosynthesized silver nanoparticles mediated by Raphanus sativus L. extracts obtained through two extraction methods (temperature and microwave) and to test their potential application for controlling apple crops pathogens. The phytosynthesized materials were characterized by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. All the materials were evaluated in terms of antioxidant and in vitro antimicrobial activity (against bacteria, molds, and yeast: Escherichia coli ATCC 8738, Staphylococcus aureus ATTC 25923, Pseudomonas aeruginosa ATCC 9027, Salmonella typhimurium ATCC 14028, Candida albicans ATCC 10231, Venturia inaequalis, Podosphaera leucotricha, Fusarium oxysporum ATCC 48112, Penicillium hirsutum ATCC 52323, and Aspergillus niger ATCC 15475). Considering the results obtained in the in vitro assays, formulations based on nanoparticles phytosynthesized using Raphanus sativus L. waste extracts (RS1N) were evaluated as potential antifungal agents for horticultural crops protection, against Venturia inaequalis and Podosphaera leucotricha through in vivo assays. For the DPPH assay, the inhibition (%) varied between 37.06% (for RS1N at 0.8 mg/mL concentration) and 83.72% (for RS1N at 7.2 mg/mL concentration) compared to 19.97% (for RS2N at 0.8 mg/mL) and only 28.91% (for RS2N at 7.2 mg/mL). Similar results were obtained for RS3N (85.42% inhibition at 7.2 mg/mL) compared with RS4N (21.76% inhibition at 7.2 mg/mL). Regarding the ABTS assay, the highest scavenger activity values were recorded for samples RS1N (91.43% at 1.6 mg/mL) and RS3N (96.62% at 1.6 mg/mL).

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Section: In Vitro Antimicrobial Activitymentioning

confidence: 99%

Section: In Vitro Antimicrobial Activitymentioning

confidence: 99%

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

Ungureanu

Fierăscu

et al. 2021

Materials

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“…The petiole was wrapped with absorbent cotton, and the small pieces of the abaxial surface of the leaves were placed in a Petri dish. Furtherly, they were cultured in a Laboshake shaker (Gerhardt GmbH & Co. KG, Königswinter, Germany) at 22 • C for 24-48 h, as previously described [7], and in concordance with literature data [52][53][54]. For the detached leaf assay, the sporangia were collected with a sterile brush.…”

Section: In Vitro Antimicrobial Studiesmentioning

confidence: 99%

“…Powdery mildew and downy mildew are among the most important diseases of grapevine [6,7]. According to literature data, powdery mildew (produced by the pathogen Uncinula necator) is the oldest grapevine disease known to science and practice.…”

Section: Introductionmentioning

confidence: 99%

Grapevine Plants Management Using Natural Extracts and Phytosynthesized Silver Nanoparticles

Vizitiu¹,

Sardarescu

Fierăscu

et al. 2022

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Starting from the well-known antimicrobial properties of silver nanoparticles, the goal of this study is to evaluate the influence of two “green” recipes, namely an alcoholic extract of Dryopteris filix-mas (L.) Schott and a dispersion of silver nanoparticles phytosynthesized using the extract on grapevine pathogens. The influence of some grapevine parameters (pith/wood rapport, soluble sugars, starch, total sugars, total water content, length of young shoots, number of grapes) in field experiments was also studied. The study was conducted on four clones (Feteasca alba 97 St., Feteasca neagra 6 St., Feteasca regala 72 St., and Cabernet Sauvignon 131 St.) located in vegetation pots inside a greenhouse. For the phytosynthesis of the silver nanoparticles (AgNPs) we used a scaled-up technology, allowing us to obtain large quantities of nanoparticles-containing solution. The AgNPs analysis by X-ray diffraction and transmission electron microscopy confirmed the synthesis of spherical and quasi-spherical nanoparticles of 17 nm average diameter and 6.72 nm crystallite size. The field experiments registered different responses of the four clones to the treatment, using both the natural extracts and phytosynthesized nanoparticles solution. Both recipes exhibited a protective effect against the Uncinula necator pathogen. For the treatment using phytosynthesized nanoparticles, significant increases in the pith/wood ratio for white wine clones (Feteasca alba 97 St. and Feteasca regala 72 St.) were observed. The biochemical analyses revealed other significant increases of soluble sugars (red wine clones—Feteasca neagra and Cabernet Sauvignon/second year), starch (Feteasca alba and Cabernet Sauvignon in 2021 for both clones), total sugars (Feteasca alba and Feteasca neagra in 2021 for both clones), and of total water content (Feteasca alba and Feteasca neagra in 2021 for both clones), respectively. The applied treatments also led to an increase of young shoots length and grape numbers for all clones as compared to the control (chemical pesticide), which would suggest a potential biostimulant effect of the recipes.

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Major Applications of Nanotechnology in Food Industry

Singh,

Kumar

2023

Nanotechnology Advancement in Agro-Food Industry

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Isolation and Cultivation of Some Pathogen Fungi from Apple and Grapevines Grown in Arges County

Cited by 7 publications

References 2 publications

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

Grapevine Plants Management Using Natural Extracts and Phytosynthesized Silver Nanoparticles

Major Applications of Nanotechnology in Food Industry

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