Isolation and culture of umbilical vein mesenchymal stem cells

Abstract

Bone marrow contains a population of stem cells that can support hematopoiesis and can differentiate into different cell lines including adipocytes, osteocytes, chondrocytes, myocytes, astrocytes, and tenocytes. These cells have been denoted mesenchymal stem cells. In the present study we isolated a cell population derived from the endothelium and subendothelium of the umbilical cord vein which possesses morphological, immunophenotypical and cell differentiation characteristics similar to those of mesenchymal stem cells isolated from bone marrow. The cells were isolated from three umbilical cords after treatment of the umbilical vein lumen with collagenase. The cell population isolated consisted of adherent cells with fibroblastoid morphology which, when properly stimulated, gave origin to adipocytes and osteocytes in culture. Immunophenotypically, this cell population was found to be positive for the CD29, CD13, CD44, CD49e, CD54, CD90 and HLA-class 1 markers and negative for CD45, CD14, glycophorin A, HLA-DR, CD51/61, CD106, and CD49d. The characteristics described are the same as those presented by bone marrow mesenchymal stem cells. Taken together, these findings indicate that the umbilical cord obtained from term deliveries is an important source of mesenchymal stem cells that could be used in cell therapy protocols.

Blastema cells derived from New Zealand white rabbit’s pinna carry stemness properties as shown by differentiation into insulin producing, neural, and osteogenic lineages representing three embryonic germ layers

“…Furthermore, according to our observations, the untreated SLCs expressed neural markers and could differentiate into neural lineage after a shorter induction period as compared with NTERA-2 cells. To investigate mesodermal differentiation of SCLs, an osteogenic medium, containing dexamethasone, ascorbate-2-phosphate and b-glycerol phosphate, was used based on several reports and our previous experience (Covas et al 2003;Ahmadian Kia et al 2011;Sun and Ji 2012). Differentiation of SLCs toward osteocytes was confirmed by their mineralized matrix stained with Alizarin Red S. RT-PCR studies also indicated the expression of Ocn and Opn in differentiated SLCs, quite similar to induced rMSCs.…”

Lipopolysaccharide Activates Toll-like Receptor 4 (TLR4)-mediated NF-κB Signaling Pathway and Proinflammatory Response in Human Pericytes

“…Cell Culture and Reagents-Human MSCs from umbilical cord (19) were purchased from Inbiobank (Inbiomed, San Sebastian, Spain) and were cultured in low glucose DMEM (Lonza, Basel, Switzerland) supplemented with 10% MSC-qualified fetal calf serum (Promocell, Heidelberg, Germany). HBVPs were purchased from ScienCell Research Laboratories (Carlsbad, CA) and cultured in 2% fetal calf serum pericyte medium (ScienCell Research Laboratories).…”

Triiodotironina não aumenta a diferenciação osteogênica reduzida pela idade de células-tronco mesenquimais da medula óssea de ratas

“…A molécula CD73 pode ser expressa tanto em fibroblastos (2) quanto em CTM (30). Mas as moléculas CD90 e CD54 expressas em mais de 86% das células deste estudo são expressas nas CTM, não sendo expressas em fibroblastos e em células hematopoéticas (2,32,33). O resultado da caracterização fenotípica das células deste estudo está de acordo com as características de CTM propostas pelo Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy (34).…”