Isolation and Diagnosis of Serratia ficaria Bacterium and Estimation of Their Production of Multiple Outpatient Sugar

Abstract

The study involved isolation and identification of the bacterium Serratia ficaria from the soil ,and The effect of incubation period , different carbon and nitrogen sources on ability of the isolate to produce extracellular polysaccharide was investigatal . The highest production of polysaccharide (2.55 g/L ) was obtained after (5) days of incubation . Maximum and lowest production of polysaccharide was achieved when glucose and sodium nitrate was ( 2.57 and 2.91 g/l) , lactose and sodium nitrite (0.72 and 0.93 g/L) as a carbon and nitrogen source respectively . ‫ﺍﻟ‬ ‫ﻤﻠﺨﺹ‬ ‫ﺘﻀﻤﻨ‬‫ﺕ‬ ‫ﺍﻟ‬ ‫ﺩﺭﺍﺴﺔ‬ ‫ﻋﺯل‬ ‫ﺠﺭﺜﻭﻤﺔ‬ Serratia ficaria ‫ﺘﺸﺨﻴﺼﻬﺎ‬ ‫ﻭ‬ ، ‫ﺍﻟﺘﺭﺒـﺔ‬ ‫ﻤـﻥ‬ ‫ﻭ‬ ‫ﺘﺄﺜﻴﺭ‬ ‫ﺩﺭﺍﺴﺔ‬ ‫ﻤﺩ‬ ‫ﺍﻟﻨﺎﻴﺘﺭﻭﺠﻴﻨﻴﺔ‬ ‫ﻭ‬ ‫ﺍﻟﻜﺎﺭﺒﻭﻨﻴﺔ‬ ‫ﺍﻟﻤﺼﺎﺩﺭ‬ ‫ﻤﻥ‬ ‫ﻤﺨﺘﻠﻔﺔ‬ ‫ﻭﺃﻨﻭﺍﻉ‬ ‫ﺍﻟﺤﻀﺎﻨﺔ‬ ‫ﺓ‬ ‫ﻓـﻲ‬ ‫ﻗﺎﺒﻠﻴـﺔ‬ ‫ﺍﻟﺨﻠﻭﻱ‬ ‫ﺍﻟﺨﺎﺭﺝ‬ ‫ﺍﻟﻤﺘﻌﺩﺩ‬ ‫ﺍﻟﺴﻜﺭ‬ ‫ﺃﻨﺘﺎﺝ‬ ‫ﻋﻠﻰ‬ ‫ﺍﻟﻌﺯﻟﺔ‬ . ‫ﺍﻥ‬ ‫ﻭﺘﺒﻴﻥ‬ ‫ﺍﻟﻤﺘﻌﺩﺩ‬ ‫ﻟﻠﺴﻜﺭ‬ ‫ﺇﻨﺘﺎﺠﻴﺔ‬ ‫ﺃﻋﻠﻰ‬ ) 2.55 ‫ﻏﻡ‬ / ‫ﻟﺘﺭ‬ ( ‫ﻜﺎﻨﺕ‬ ‫ﺒﻌﺩ‬ ) 5 ‫ﺃﻴﺎﻡ‬ ( ‫ﺍﻟﺘﺤﻀﻴﻥ‬ ‫ﻤﻥ‬ ، ‫ﺒﻴﻨﻤﺎ‬ ‫ﺃﻗﺼﻰ‬ ‫ﺘﺤﻘﻘﺕ‬ ‫ﻭﺍﻗل‬ ‫ﺍﻟﻤﺘﻌﺩﺩ‬ ‫ﻟﻠﺴﻜﺭ‬ ‫ﺇﻨﺘﺎﺠﻴﺔ‬ ‫ﺍﻟﺼﻭﺩﻴﻭﻡ‬ ‫ﻭﻨﺘﺭﺍﺕ‬ ‫ﺍﻟﻜﻠﻭﻜﻭﺯ‬ ‫ﺍﺴﺘﺨﺩﺍﻡ‬ ‫ﻋﻨﺩ‬ ) 2.57 ‫ﻭ‬ 2.91 ‫ﻏﻡ‬ / ‫ﻟﺘﺭ‬ ( ‫ﻭ‬ ‫ﻭﻨﺘﺭﻴـﺕ‬ ‫ﺍﻟﻼﻜﺘﻭﺯ‬ ‫ﺍﻟﺼﻭﺩﻴﻭﻡ‬ ) 0.72 ‫ﻭ‬ 0.93 ‫ﻏﻡ‬ / ‫ﻟﺘﺭ‬ ( ‫ﺒﻭﺼﻔﻪ‬ ‫ﻤﺼﺩﺭ‬ ‫ﹰ‬ ‫ﺍ‬ ‫ﻭﺍﻟﻨ‬ ‫ﻟﻠﻜﺎﺭﺒﻭﻥ‬ ‫ﺎﻴ‬ ‫ﺍﻟﺘﻭﺍﻟﻲ‬ ‫ﻋﻠﻰ‬ ‫ﺘﺭﻭﺠﻴﻥ‬ . ‫ﺍﻟ‬ ‫ﻤﻘﺩﻤﺔ‬ ‫ﺘﻨـﺘﺞ‬ ‫ﺍﻟﺘـﻲ‬ ، ‫ﹰ‬ ‫ﺤﺩﻴﺜﺎ‬ ‫ﺍﻟﻤﻜﺘﺸﻔﺔ‬ ‫ﺍﻟﻨﺘﺎﺠﺎﺕ‬ ‫ﺃﻫﻡ‬ ‫ﺃﺤﺩﻯ‬ ‫ﺍﻟﺠﺭﺜﻭﻤﻴﺔ‬ ‫ﺍﻟﻤﺘﻌﺩﺩﺓ‬ ‫ﺍﻟﺴﻜﺭﻴﺎﺕ‬ ‫ﺘﻌﺩ‬ ‫ﺍﻟﻤﺨﻤﺭﺍﺕ‬ ‫ﺒﺎﺴﺘﺨﺩﺍﻡ‬ ‫ﻜﺒﻴﺭﺓ‬ ‫ﻭﺒﻜﻤﻴﺎﺕ‬ Fermentars ‫ﺍﻟ‬ ‫ﻴﻌﺩ‬ ‫ﺍﻟﺴﻜﺭﻴﺎﺕ‬ ‫ﻫﺫﻩ‬ ‫ﺘﻭﺍﺠﺩ‬ ‫ﻭﺍﻥ‬ ، ‫ﺼﻨﺎﻋﻴﺔ‬ ‫ﺍﻟﺨﻼﻴﺎ‬ ‫ﺩﺍﺨل‬ ‫ﺘﻜﻭﻥ‬ ‫ﺍﻥ‬ ‫ﺍﻤﺎ‬ ‫ﻓﻬﻲ‬ ‫ﺘﺼﻨﻴﻔﻬﺎ‬ ‫ﻓﻲ‬ ‫ﹰ‬ ‫ﺃﺴﺎﺴﺎ‬ ) intracellular ( ‫ﺍ‬ ‫ﺘﺭﻜﻴـﺏ‬ ‫ﻓـﻲ‬ ‫ﺘﺩﺨل‬ ‫ﻭ‬ ‫ﺨﻼﻴﺎﻫﺎ‬ structural ‫ﺍﻟﺨﻠﻴﺔ‬ ‫ﺨﺎﺭﺝ‬ ‫ﺇﻟﻰ‬ ‫ﺘﻔﺭﺯ‬ ‫ﺍﻭ‬ ) extracellular ( . ‫ﺘﻜﻭﻥ‬ ‫ﺍﻷﺨﻴﺭﺓ‬ ‫ﻫﺫﻩ‬ ‫ﻭﺍﻥ‬