Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Chen, Yu-Jen; Liu, Huiyu; Chang, Ya‐Hsuan; Cheng, Ying-Hung; Mersmann, Harry J.; Kuo, Wen‐Hung; Ding, Shih‐Torng

doi:10.3791/53886

Cited by 38 publications

(35 citation statements)

References 34 publications

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“…Expression of sterol regulatory element-binding protein 1 (SREBP1c; a transcription factor associated with lipogenesis) is inhibited, and acyl CoA oxidase (ACOX; promoting fatty acid oxidation) expression is increased in liver by DHA treatment [133]. To exclude the confounding endocrine effects by other organs in vivo, a primary porcine adipocyte system has been established [134] to investigate DHA effects. The expression of SREBP1c is inversely correlated with DHA treatment, whereas ACOX is positively correlated with DHA concentrations.…”

Section: The Beneficial Physiological Effects Of N-3 Pufamentioning

confidence: 99%

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Role of n-3 Polyunsaturated Fatty Acids in Ameliorating the Obesity-Induced Metabolic Syndrome in Animal Models and Humans

Huang

Chien

Chen

et al. 2016

IJMS

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The incidence of obesity and its comorbidities, such as insulin resistance and type II diabetes, are increasing dramatically, perhaps caused by the change in the fatty acid composition of common human diets. Adipose tissue plays a role as the major energy reservoir in the body. An excess of adipose mass accumulation caused by chronic positive energy balance results in obesity. The n-3 polyunsaturated fatty acids (n-3 PUFA), DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid) exert numerous beneficial effects to maintain physiological homeostasis. In the current review, the physiology of n-3 PUFA effects in the body is delineated from studies conducted in both human and animal experiments. Although mechanistic studies in human are limited, numerous studies conducted in animals and models in vitro provide potential molecular mechanisms of the effects of these fatty acids. Three aspects of n-3 PUFA in adipocyte regulation are discussed: (1) lipid metabolism, including adipocyte differentiation, lipolysis and lipogenesis; (2) energy expenditure, such as mitochondrial and peroxisomal fatty acid β-oxidation; and (3) inflammation, including adipokines and specialized pro-resolving lipid mediators. Additionally, the mechanisms by which n-3 PUFA regulate gene expression are highlighted. The beneficial effects of n-3 PUFA may help to reduce the incidence of obesity and its comorbidities.

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Section: The Beneficial Physiological Effects Of N-3 Pufamentioning

confidence: 99%

“…Compared to most rodent models, adipose tissue depots in pigs are of sufficient size for stromal vascular or adipocyte preparation without pooling across depots or animals [116,134]. These systems in vitro are effective for studying the PUFA functions in lipid metabolism.…”

Section: The Molecular Mechanisms By Which Pufa Affects Lipid Metamentioning

confidence: 99%

Role of n-3 Polyunsaturated Fatty Acids in Ameliorating the Obesity-Induced Metabolic Syndrome in Animal Models and Humans

Huang

Chien

Chen

et al. 2016

IJMS

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“…Unlike other types of adult stem cells, ADSCs can be easily obtained with minimal harm to donors and expanded in vitro with well‐established procedures (Chen et al, ). By analyzing the differentiation potential and surface markers, we validated the procedure for isolating ADSCs.…”

Section: Introduction Results and Discussionmentioning

confidence: 99%

Base excision repair but not DNA double‐strand break repair is impaired in aged human adipose‐derived stem cells

et al. 2019

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The decline in DNA repair capacity contributes to the age-associated decrease in genome integrity in somatic cells of different species. However, due to the lack of clinical samples and appropriate tools for studying DNA repair, whether and how age-associated changes in DNA repair result in a loss of genome integrity of human adult stem cells remains incompletely characterized. Here, we isolated 20 eyelid adipose-derived stem cell (ADSC) lines from healthy individuals (young: 10 donors with ages ranging 17-25 years; old: 10 donors with ages ranging 50-59 years). Using these cell lines, we systematically compared the efficiency of base excision repair (BER) and two DNA double-strand break (DSB) repair pathways-nonhomologous end joining (NHEJ) and homologous recombination (HR)-between the young and old groups.Surprisingly, we found that the efficiency of BER but not NHEJ or HR is impaired in aged human ADSCs, which is in contrast to previous findings that DSB repair declines with age in human fibroblasts. We also demonstrated that BER efficiency is negatively associated with tail moment, which reflects a loss of genome integrity in human ADSCs. Mechanistic studies indicated that at the protein level XRCC1, but not other BER factors, exhibited age-associated decline. Overexpression of XRCC1 reversed the decline of BER efficiency and genome integrity, indicating that XRCC1 is a potential therapeutic target for stabilizing genomes in aged ADSCs. K E Y W O R D Sadipose-derived stem cells, base excision repair, genome integrity, human aging, XRCC1

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“…The method was that of Chen et al, 2016 [27]. Briefly, cells were isolated from adipose tissue from 7 to 9-day old pigs using collagenase digestion.…”

Section: Preparation Of Porcine Adipose Tissue Derived Stem Cellsmentioning

confidence: 99%

Modulation of Fatty Acid Oxidation and Glucose Uptake by Oxytocin in Adipocytes

Lin¹,

Chen²,

Chen³

et al. 2017

JBiSE

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Oxytocin (OT) is a hypothalamic neuropeptide synthesized and secreted by OT neurons. In addition to its conventional role in reproductive physiology, central OT also regulates various social behaviors, such as care, trust, and emotions. Central and subcutaneous OT infusions stimulate lipid metabolism in mice and rats when fed standard or high fat diets. Mice lacking the OT receptor (OTR) or OT peptide develop late-onset obesity with greater fat pad weights, larger adipocyte size and elevated plasma levels of leptin. To study the effects of OT on lipid metabolism, we examined the effects of serial OT doses (0, 10, 30, 100, 150, 300 nM) on 3T3L1 adipocytes, together with long (144 hours, 6 days) and short (24 hours, 1 day) term treatments. The shortterm treatment with 150 nM OT increased triacylglycerol (TAG) accumulation and decreased mRNA expressions of carnitine palmitoyltransferase 1α (CPT-1α) and fatty acid binding protein 4 (FABP4). After long-term incubation with 150 nM OT, only the CPT-1α mRNA was decreased. In differentiated adipocytes derived from pig adipose tissue stem cells, only hormone sensitive lipase mRNA was decreased after short-or long-term treatment with OT. To obtain further insight into the underlying mechanism of OT induction, we tested the involvement of the AKT/PKB pathway; however, AKT phosphorylation was decreased after treatment with 150 nM OT, suggesting that OT effects may be independent from the AKT/PKB pathway. Taken together, OT effects on adipocyte glucose and lipid metabolism are probably through mechanisms other than the AKT/PKB pathway.

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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Cited by 38 publications

References 34 publications

Role of n-3 Polyunsaturated Fatty Acids in Ameliorating the Obesity-Induced Metabolic Syndrome in Animal Models and Humans

Role of n-3 Polyunsaturated Fatty Acids in Ameliorating the Obesity-Induced Metabolic Syndrome in Animal Models and Humans

Base excision repair but not DNA double‐strand break repair is impaired in aged human adipose‐derived stem cells

Modulation of Fatty Acid Oxidation and Glucose Uptake by Oxytocin in Adipocytes

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