Isolation and differentiation of herpes simplex virus and Trichomonas vaginalis in cell culture

Gentry, Glenn A.; Lawrence, Nancy; Lushbaugh, William B.

doi:10.1128/jcm.22.2.199-204.1985

Cited by 16 publications

(8 citation statements)

References 23 publications

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“…Krieger et al (18) also studied the effects of filtered extracts of sonicated T. vaginauli cells and failed to detect a cytotoxic effect. This is in contrast to the findings of Gentry et al (12), who demonstrated a cytopathic effect of T. vaginalis lysates. Gentry et al (12) also found that the cytopathic effect of the lysates was inhibited by serum and was dependent on the amount of protein present in the lysate.…”

Section: Discussioncontrasting

confidence: 99%

“…This is in contrast to the findings of Gentry et al (12), who demonstrated a cytopathic effect of T. vaginalis lysates. Gentry et al (12) also found that the cytopathic effect of the lysates was inhibited by serum and was dependent on the amount of protein present in the lysate. In the presence of less protein, a longer incubation time was needed to demonstrate the same cytopathogenicity.…”

Section: Discussioncontrasting

confidence: 99%

“…By 6 h, undiluted filtrate disrupted the monolayer, whereas at all other dilutions the monolayer remained confluent. At 12 h, there was a significant correlation between the dilution of the filtrate and the effect over time on the confluence of the monolayer (r = and 24 h after addition of the filtrates (curves T(, T3, T6, Tl, and T2., respectively). At 12 h, there was a significant correlation between the dilution of the filtrate and the effect on monolayer confluence (r = 0.95, P < 0.01).…”

Section: Cdf Activity On Monolayers Coincubation Of Confluentmentioning

confidence: 93%

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Isolation of a cell-detaching factor of Trichomonas vaginalis

1989

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The pathogenetic role of soluble products of Trichomonas vaginalis growth in culture is controversial. To evaluate this role, T. vaginalis was grown in broth and cell culture and the cell-free filtrate was applied to fresh cell culture monolayers. When adjusted to pH 6.5, filtrates obtained from 22-h culture growth totally disrupted McCoy, HEp-2, human foreskin fibroblast, and Chinese hamster ovary cell monolayers within 6 h. These detached ceils remained >90% viable. This cell-detaching factor (CDF) was heat and acid labile, with a pH optimum of 6.5. CDF has trypsinlike activity which disrupts monolayer cells, but cells do not die if the pH is controlled. CDF was purified by ethanol precipitation, ammonium sulfate fractionation, and ion-exchange and gel filtration column chromatography. A 200,000-molecular-weight glycoprotein which was also immunogenic by immunoblot with human sera reactive to T. vaginalis was isolated in this manner. This confirms the presence of a specific soluble CDF derived from T. vaginalis whose application may be important as a diagnostic tool and in further studies of pathogenesis.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Section: Cdf Activity On Monolayers Coincubation Of Confluentmentioning

confidence: 93%

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Isolation of a cell-detaching factor of Trichomonas vaginalis

1989

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“…Previous investigations using large inocula have shown that T. vaginalis will disrupt a cell culture monolayer (1, 4,9,10,14). However, by comparing the growth of T. vaginalis in McCoy cell culture to growth in the same system without McCoy cells, the present study demonstrated that the presence of eucaryotic cells appears to enhance the growth of T. vaginalis.…”

Section: Discussioncontrasting

confidence: 61%

“…Despite this, clinical and laboratory diagnosis of trichomoniasis, especially in men, may sometimes be difficult (12). T. vaginalis will grow in cell culture (1, 4, 9, 10, 14) but has infrequently been grown in cell culture from clinical specimens that had not been frozen before processing (9,14). As shown in this report, in vitro cell culture was more sensitive than broth in detecting low numbers of inoculated T. vaginalis.…”

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confidence: 82%

Cell culture compared with broth for detection of Trichomonas vaginalis

Garber¹,

Sibau²,

Ma³

et al. 1987

J Clin Microbiol

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Trichomonas vaginalis can be grown in cell culture. We studied the growth kinetics of T. vaginalis in McCoy cell culture compared with that in a conventional broth medium (Diamond TYI-S-33 medium supplemented with 10% heat-inactivated bovine serum [TYI]). In the presence of McCoy cells and two parts cell culture medium to one part TYI, a peak concentration of 2 x 106 to 6 x 106 T. vaginalis per ml was consistently achieved with inocula as low as three T. vaginalis cells per ml. Without cells, this medium did not support growth of T. vaginalis. T. vaginalis in TYI in 1-ml vials with or without McCoy cells demonstrated poor growth. In tubes containing 10 ml of TYI, inocula grew to 2 x 106 to 6 x 106 T. vaginalis per ml, but at least 3 x 101 T. vaginalis per tube was required to initiate growth. Thus, in vitro, cell culture was more sensitive than TYI broth in detecting low numbers of T. vaginalis. In a subsequent clinical comparison of broth and cell culture for isolation of T. vaginalis from 188 vaginal specimens and 21 urethral specimens from men, the results were in agreement for 206 specimens (98.6%). There were no situations in which culture was negative and a saline preparation showed motile trichomonads. For women, using a positive culture as the indicator of true positivity, the sensitivity of detection of T. vaginalis was 83% with the Pappenheim stain and 77% with saline preparations. In tests with a limited number of men, the Pappenheim stain was neither sensitive nor specific for detection of T. vaginalis in comparison with cultures or saline preparations. These studies show that cell culture can be used for isolation of T. vaginalis from clinical specimens; it gave results comparable to those of broth culture for the group of mainly symptomatic womèn. Further studies should be performed to determine its utility in clinical populations such as asymptomatic women and men with and without symptoms, in which T. vaginalis is more likely to be present in low nuffibers.

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Host Cell-Trichomonad Interactions and Virulence Assays Using in In Vitro Systems

Honigberg¹

1990

Trichomonads Parasitic in Humans

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Isolation and differentiation of herpes simplex virus and Trichomonas vaginalis in cell culture

Cited by 16 publications

References 23 publications

Isolation of a cell-detaching factor of Trichomonas vaginalis

Isolation of a cell-detaching factor of Trichomonas vaginalis

Cell culture compared with broth for detection of Trichomonas vaginalis

Host Cell-Trichomonad Interactions and Virulence Assays Using in In Vitro Systems

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