Isolation and genetic characterization of &lt;em&gt;Lysinibacillus sphaericus&lt;/em&gt; strains found in mosquito larvae (Diptera: Culicidae)

Cavados, Clara de Fátima Gomes; Pires, Eder S.; Chaves, Jeane Q.; Alvarez, Danielle Nunes; Gil, Hélio Benites; Oliveira, Iris Braz Ribeiro de; Cunha, Andrea de Barros Pinto Viviani; Araújo-Coutinho, Carlos José Pereira da Cunha de

doi:10.2147/rrtm.s124066

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…and Anopheles sp. [8,18] The morphology of L. sphaericus observed using phase-contrast microscopic techniques has the characteristics of a sphaerical (round) shape, spores located terminally, and its sporangia are swollen. In contrast, B. thuringiensis has the characteristics of broad bacterial cells with spore-shaped ellipses, spores situated sub-terminally, un-swollen sporangia and the protein crystals appear darker than the spores [16].…”

Section: Morphological Characteristic Of Protein Crystalmentioning

confidence: 99%

Larvicide effects of Bacillus sp isolated from soil against fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

Handayani¹,

Janah²,

Ekowati³

et al. 2023

GSC Biol. Pharm. Sci.

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Prolonged use of pesticides was known to have a negative impact on the environment, health, and living organisms. Therefore alternative pest control techniques need to be developed continuously. One pest eradication techniques that is believed to be safe for the environment is biological control. This study intended to evaluate the toxicity properties of 7 different isolates suspension of Bacillus sp. isolated from soil in a botanical garden in West Lampung against Spodoptera frugiperda larvae. Morphological characterization of protein crystals which are toxic to insects contained in the suspension was carried out microscopically at a magnification of 1000x. Then, each isolate suspension was tested for its toxicity properties on the 3rd instar larvae of S. frugiverda. The protein crystal morphology of the Bacillus sp isolate coded TB7 was similar to that of L. sphaericus. While the other six isolates (TMA 26, TBA 4, TBA 7, TB 5, BP 14, and TSR 6) had morphological characteristics similar to that found in the B. thuringiensis. The results of larvicide test showed that Bacillus sp isolates coded TMA 26, TBA 7, BP 14 and TB 5 caused mortality on day 3. TBA 4 isolates killed larvae on day 4, while isolates coded TSR 6 and TB 7 killed larvae on day 5. In conclusion, Bacillus sp isolate isolated from soil has toxic properties to the larvae of S frugiperda. So it is suggested that the bacterial isolate Bacillus sp can be used as a biological control agent for insect pests.

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Section: Morphological Characteristic Of Protein Crystalmentioning

confidence: 99%

Larvicide effects of Bacillus sp isolated from soil against fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

Handayani¹,

Janah²,

Ekowati³

et al. 2023

GSC Biol. Pharm. Sci.

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Diversity of free-living amoebae in soils and their associated human opportunistic bacteria

et al. 2017

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Free-living amoebae (FLA) are ubiquitous protozoa found worldwide in the environment. They feed by phagocytosis on various microorganisms. However, some bacteria, i.e., amoebae-resistant bacteria (ARB) or bacterial endocytobionts, can resist phagocytosis and even multiply inside FLA. This study investigated the diversity of culturable FLA in various soils from agricultural and mining sites and their bacterial endocytobionts. FLA were cultured on non-nutrient agar with alive Escherichia coli and identified by PCR and sequencing. Amoebae were lysed and bacterial endocytobionts were cultured on TSA 1/10 and Drigalski medium. Bacterial isolates were identified by PCR and 16S rDNA sequencing and characterized for their antibiotic resistance properties. To measure bacterial virulence, the amoebal model Dictyostelium discoideum was used. The analysis of FLA diversity showed that Tetramitus was the most prevalent genus in agricultural soil from Burkina Faso (73%) and garden soil from Vietnam (42%) while Naegleria and Acanthamoeba were dominant genera in mining soil from Vietnam (55%) and French alpine soil (77%). Some genera were only present in one out of the four soils analyzed. The bacterial endocytobiont included Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria. Human opportunistic pathogens identified as Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Burkholderia cepacia were found associated with amoebae including Micriamoeba, Tetramitus, Willaertia, or Acanthamoeba. Some of these bacteria showed various antibiotic resistance phenotypes and were virulent. Our study confirms that the occurrence of these opportunistic bacteria with FLA in soils may be important for the survival, multiplication, and spread of pathogens in the environment.

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Lysinibacillus spp.: an IAA-producing endospore forming-bacteria that promotes plant growth

Pantoja-Guerra

Burkett-Cadena

Cadena

et al. 2023

Antonie van Leeuwenhoek

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Lysinibacillus is a bacterial genus that has generated recent interest for its biotechnological potential in agriculture. Strains belonging to this group are recognized for their mosquitocidal and bioremediation activity. However, in recent years some reports indicate its importance as plant growth promoting rhizobacteria (PGPR). This research sought to provide evidence of the PGP activity of Lysinibacillus spp. and the role of the indole-3-acetic acid (IAA) production associated with this activity. Twelve Lysinibacillus spp. strains were evaluated under greenhouse conditions, six of which increased the biomass and root architecture of corn plants. In most cases, growth stimulation was evident at 108 CFU/mL inoculum concentration. All strains produced IAA with high variation between them (20–70 µg/mL). The bioinformatic identification of predicted genes associated with IAA production allowed the detection of the indole pyruvic acid pathway to synthesize IAA in all strains; additionally, genes for a tryptamine pathway were detected in two strains. Extracellular filtrates from all strain’s cultures increased the corn coleoptile length in an IAA-similar concentration pattern, which demonstrates the filtrates had an auxin-like effect on plant tissue. Five of the six strains that previously showed PGPR activity in corn also promoted the growth of Arabidopsis thaliana (col 0). These strains induced changes in root architecture of Arabidopsis mutant plants (aux1-7/axr4-2), the partial reversion of mutant phenotype indicated the role of IAA on plant growth. This work provided solid evidence of the association of Lysinibacillus spp. IAA production with their PGP activity, which constitutes a new approach for this genus. These elements contribute to the biotechnological exploration of this bacterial genus for agricultural biotechnology.

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Isolation and genetic characterization of <em>Lysinibacillus sphaericus</em> strains found in mosquito larvae (Diptera: Culicidae)

Cited by 4 publications

References 13 publications

Larvicide effects of Bacillus sp isolated from soil against fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

Larvicide effects of Bacillus sp isolated from soil against fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

Diversity of free-living amoebae in soils and their associated human opportunistic bacteria

Lysinibacillus spp.: an IAA-producing endospore forming-bacteria that promotes plant growth

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