Serratia marcescens strain MBC1 merupakan bakteri gram negatif yang dapat menghasilkan beberapa senyawa bioaktif. Berdasarkan penelitian sebelumnya, diketahui bahwa bakteri ini mampu mendegradasi berbagai macam enzim dan memiliki berbagai macam aktivitas biologis seperti antibakteri, antikanker, biosurfaktan dan sebagai bahan obat-obatan. Tujuan penelitian ini adalah untuk mengetahui kandungan senyawa bioaktif yang terdapat pada ekstrak metabolit sekunder Serratia marcescens strain MBC1. Pada penelitian ini dilakukan beberapa uji yaitu, uji fitokomia dan uji FT-IR. Hasil uji fitokimia menunjukkan bahwa ekstrak Serratia marcescens strain MBC1 mengandung senyawa golongan alkaloid dan saponin. Hasil uji FT-IR menunjukkan adanya kemiripan gugus fungsi yang dimiliki oleh ekstrak S.marcescens strain MBC1 dengan senyawa golongan alkaloid. Namun, perlu adanya penelitian lebih lanjut mengenai kandungan senyawa metabolit yang terkandung dalam ekstrak S. marcescens dan aktivitas biologisnya sebagai antimalaria, antibakteri, antifungi dan sebagai bahan obat-obatan.
Hydrolase enzymes are a class of enzymes used to break down the organic substrates into simpler molecules. Hydrolase enzymes play a role in biocontrol, industry, and public health. One of the hydrolase enzyme producers is bacteria. Serratia marcescens strain MBC1 is a collection in the Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung which enzymatic activities haven’t been known yet. This research aimed to determine the enzymatic activity of S. marcesescens strain MBC1 that can be used as a candidate for biocontrol agents, biosurfactant producers, industry, or in the health sector. The screening was carried out using a qualitative method plate assay using selective solid media for each test, with three replications for each test. The data obtained were analyzed statistically using analysis non-parametric kruskall-wallis test. The lipolytic activity of the S. marcescens strain MBC1 showed high efficacy with an enzymatic index of 5.52 and amylase, cellulases, protease, mannanase, and chitinase with enzymatic indexes, respectively, 4.17, 1.50, 0.69, 0.35, and 0.27. This enzyme activity of this isolate can be used as a candidate for biological control agents,biosurfactant producers, industry, or the health sector.
Minyak jelantah (mijel) is known in Indonesia as waste cooking oil. Even though it is a waste, mijel is still used by consumers for savings. The problem is, if it is used repeatedly more than three times, mijel can cause health problems. In addition to health hazards, if discharged through water channels, it can cause environmental pollution. Lack of public knowledge about the dangers of using the mijel in the end will have a negative impact. To educate the public about the health-risks of using mijel, we held a short training for housewives in Kelurahan Tanjung Agung, Bandar Lampung on July 22, 2020. This training provided the targeted housewives with the knowledge and skills to process the waste cooking oil into laundry soap. The purpose of this activity was to educate and provide training to the community, especially PKK women who interact directly with the use of mijel, about the dangers of mijel and how to manage it. PKK women who participated in the training seemed interested and understood the explanation described, this can be seen from the increase in the average pretest score from 55.7 to 81.3 at posttest. The benefits obtained by the participants are: 1) Understanding that using mijel more than three times can cause health problems for consumers. 2) Processing of waste cooking oil to laundry soap can reduce pollution in the aquatic environment.3) Laundry soap made from waste cooking oil can be a source of family income.
Minyak goreng bekas yang sudah berwarna coklat kehitaman sebaiknya tidak digunakan lagi karena bersifat karsinogenik. Minyak goreng yang telah dipakai berulang kali biasa disebut dengan minyak jelantah. Biasanya minyak jelantah (mijel) akan dibuang oleh ibu-ibu secara sembarangan sehingga menyebabkan minyak tersebut menjadi limbah di lingkungan sekitar. Kegiatan ini bertujuan untuk mengedukasi ibu-ibu mengenai bahaya penggunaan mijel secara berulang kali serta membantu memberikan solusi agar mijel dapat dimanfaatkan kembali dalam bentuk lain, salah satunya dibuat sabun cuci. Peserta kegiatan ini adalah ibu-ibu PKK di Kelurahan Labuan Ratu Raya Bandar Lampung, sebanyak 13 orang. Kegiatan ini terdiri dari, diskusi, demonstrasi dan praktik langsung. Hasil kegiatan pengabdian ini menunjukan bahwa para peserta sangat antusias dan menyimak selama proses berlangsung, hal ini dilihat dari peningkatan nilai pretest dari 61,53 menjadi 81,92 dan aktifnya peserta saat melakukan praktik. Praktik dan demonstrasi dimaksudkan untuk memberikan ketrampilan kepada para peserta dalam menentukan formula yang tepat dalam pembuatan sabun cuci dari mijel. Setelah kegiatan ini berlangsung diharapkan ibu-ibu akan semakin sadar dengan bahaya penggunaan mijel dan bisa memanfaatkan mijel menjadi produk yang lebih bermanfaat.
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