Isolation and genetic characterization of enterovirus in patients with febrile rash illness

Ni, Zhaohui; Xiang, Fengyang; Huang, Honglan; Wang, Guoqing; Фан, Ли

doi:10.3892/br.2015.441

Cited by 1 publication

(1 citation statement)

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“…Sequencing of the VP1 gene showed 92%-97% similarity with strains from Taiwan that were also isolated in 2008 [20]. Recently, Ni et al reported that CVB3 isolated from patients with febrile rash illness in China was closely associated with the CVB3/MKP and CVB3/Macocy strains, which induce myocarditis and central nervous system disease [21].…”

Section: Discussionmentioning

confidence: 99%

Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness

Wiyatno

Antonjaya

Ma’roef

et al. 2016

J Infect Dev Ctries

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Introduction: Coxsackievirus B3 (CVB3) virus has been implicated as the causative agent of various outbreaks of clinical disease, including hand, foot, and mouth diseases, aseptic meningitis, acute myocarditis, and inflammatory cardiomyopathy. Methodology: Two hundred and nine undiagnosed cryopreserved specimens obtained from factory workers in Bandung, Indonesia, who displayed symptoms of acute febrile illness were gathered. Total RNA was isolated from serum and tested by conventional polymerase chain reaction (PCR) using Enterovirus genus-level primers and confirmed by sequencing. Concurrently, the virus was isolated in LLC-MK2 cells. Results: CVB3 virus was identified in an archived specimen from a patient who presented with symptoms of fever, headache, myalgia, and nausea. Sequencing results of the VP1 region from both the clinical sample and tissue culture supernatant showed 97% homology to a CVB3 virus isolate from Taiwan. Virus propagation in LLC-MK2 cell culture exhibited severe cytopathic effects two days post-inoculation. Conclusions: We report the first case of CVB3 from an undifferentiated febrile illness specimen from Indonesia.

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Section: Discussionmentioning

confidence: 99%