Isolation and Genomic Analysis of the Phage vB_PaeP_fHoPae04 Infecting Pseudomonas aeruginosa

Patpatia, Sheetal; Yilmaz, Ozgenur; Ylänne, Matti; Kiljunen, Saija

doi:10.1128/mra.00076-21

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…The discovery of phage HZ2201 can help broaden the research related to phages. The lysozyme exhibiting the phage lysis function is 100% similar to the lytic PA phage vB_PaeP_fHoPae04, and HZ2201 is a lytic phage from the genetic perspective ( Patpatia et al., 2021 ). In addition, HZ2201 forms clear spots and no lysogenicity-related genes were found in the genome of phage HZ2201, suggesting that HZ2201 is a novel lytic PA phage ( Essoh et al., 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Fei,

Li,

Liu

et al. 2023

Virus Research

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Section: Discussionmentioning

confidence: 99%

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Fei,

Li,

Liu

et al. 2023

Virus Research

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“…Pseudomonas phages have been isolated from a variety of environmental sources, including sewage [10], soil [6], lakes [11], rivers [12], and wastewater [13], and have been successfully applied in biological control. For example, the biocontrol agent phage Eir4 has been found to be efficient against Pst in vitro [2].…”

Section: Introductionmentioning

confidence: 99%

Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave

Wu,

An,

Fang

et al. 2024

Virus Genes

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Pseudomonas syringae is a gram-negative plant pathogen that infects plants such as tomato and poses a threat to global crop production. In this study, a novel lytic phage infecting P. syringae pv. tomato DC3000, named phage D6, was isolated and characterized from sediments in a karst cave. The latent period of phage D6 was found to be 60 min, with a burst size of 16 plaque-forming units per cell. Phage D6 was stable at temperatures between 4 and 40 °C but lost infectivity when heated to 70 °C. Its infectivity was unaffected at pH 6–10 but became inactivated at pH ≤ 5 or ≥ 12. The genome of phage D6 is a linear double-stranded DNA of 307,402 bp with a G + C content of 48.43%. There is a codon preference between phage D6 and its host, and the translation of phage D6 gene may not be entirely dependent on the tRNA library provided by the host. A total of 410 open reading frames (ORFs) and 14 tRNAs were predicted in its genome, with 92 ORFs encoding proteins with predicted functions. Phage D6 showed low genomic similarity to known phage genomes in the GenBank and Viral sequence databases. Genomic and phylogenetic analyses revealed that phage D6 is a novel phage. The tomato plants were first injected with phage D6, and subsequently with Pst DC3000, using the foliar spraying and root drenching inoculum approach. Results obtained after 14 days indicated that phage D6 inoculation decreased P. syringae-induced symptoms in tomato leaves and inhibited the pathogen’s growth in the leaves. The amount of Pst DC3000 was reduced by 150- and 263-fold, respectively. In conclusion, the lytic phage D6 identified in this study belongs to a novel phage within the Caudoviricetes class and has potential for use in biological control of plant diseases.

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“…Therefore, phage biocontrol using obligatory lytic phages to kill the host has been attracted attention as a potential treatment option for bacterial infections [6,9]. Currently, Pseudomonas phages have been reported to be isolated from various environmental sources such as sewage [10], soil [6], lake [11], river [12] and wastewater [13], and have been successfully applied to biological control, for example, a biocontrol agent potentially used for prevention of Pst associated phage Eir4 was found to be e cient against Pst in vitro [2], phage PPPL − 1 reported to e ciently control kiwifruit canker caused by P. syringae pv. actinidiae [14].…”

Section: Introductionmentioning

confidence: 99%

Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave

Fang

et al. 2023

Preprint

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Pseudomonas syringae is a gram-negative plant pathogen that generally infects plants such as tomato and threatens global crop production. In this study, a novel lytic phage (named Pseudomonas phage D6) infecting P. syringae pv. tomato DC3000 was isolated from the karst cave sediments and characterized. The latent period of phage D6 was shown to be 60 min, and the burst size was 16 plaque forming units/cell. Phage D6 was stable at 30 − 40°C and lost infectivity after heated to 70°C. The infectivity of phage D6 was unaffected at pH 6 − 10, but was inactivated at pH ≤ 5 or ≥ 12. The genome of phage D6 is a linear double-stranded DNA of 307,402 bp with a G + C content of 48.43%. It showed low similarity to known phage genomes in the GenBank database and Viral sequence database. Genomic and phylogenetic analyses revealed that phage D6 is a newly discovered phage. A total of 410 open reading frames (ORFs) and 14 tRNAs were predicted in the genome, 92 of which encode for proteins with predicted functions. In conclusion, the lytic phage D6 identified in this study belongs to a novel phage of the class Caudoviricetes, and has potential application in biological control of plant diseases.

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Isolation and Genomic Analysis of the Phage vB_PaeP_fHoPae04 Infecting Pseudomonas aeruginosa

Abstract: Here, we report the genomic sequence of Pseudomonas aeruginosa phage vB_PaeP_fHoPae04, isolated from hospital wastewater in Helsinki, Finland. The phage genome is 45,491 bp long, has a G+C content of 52.2%, and contains 70 protein-coding genes and 3 tRNA genes.

Cited by 4 publications

References 15 publications

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave

Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave

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