Isolation and genomic characterization of a canine parainfluenza virus type 5 strain in China

Liu, Caihong; Li, Xiangdong; Zhang, Jianpo; Yang, Linxia; Li, Fan; Deng, Junhua; Tan, Feifei; Sun, Ming; Liu, Yuxiu; Tian, Kegong

doi:10.1007/s00705-017-3387-0

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…There is some confusion in the literature with regard to the identity of CPIV. While the virus has been often referred to simply as canine parainfluenza virus, without a reference to its taxonomic classification, the majority (if not all) of CPIV that have been characterised or sequenced were parainfluenza virus (PIV) type 5-like viruses (Ellis and Krakowka 2012;Oem et al 2015;Liu et al 2017). PIV-5 is currently classified within species Mammalian rubulavirus 5, formerly Parainfluenza virus 5 (Anonymous 2017).…”

Section: Discussionmentioning

confidence: 99%

A survey of canine respiratory pathogens in New Zealand dogs

Sowman

Cave

Dunowska

2018

New Zealand Veterinary Journal

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AIMS To determine which of the common canine respiratory pathogens circulate among selected populations of healthy and diseased dogs in New Zealand. METHODS Coagulated blood samples for serology and oropharyngeal swabs for virology were collected from healthy dogs (n=47) and from dogs with acute respiratory disease (n=49). For diseased dogs a convalescent blood sample was also collected 3-4 weeks later. Oropharyngeal swabs were subjected to virus isolation and tested for canine parainfluenza virus (CPIV), canine adenovirus (CAdV) 2, canine herpesvirus (CHV), canine respiratory coronavirus (CRCoV), canine influenza virus (CIV), canine distemper virus (CDV), Bordetella bronchiseptica, Streptococcus equi subsp. zooepidemicus, and Mycoplasma cynos nucleic acids by quantitative PCR (qPCR). Sera were tested for CRCoV antibody using competitive ELISA and results expressed as percent of inhibition (POI). RESULTS The mean age of diseased dogs (2.7, min <0.5, max 8.5 years) was lower than the mean age of healthy dogs (5.3, min <0.5, max 17 years) (p<0.001). In total, 20/94 (21%) dogs were positive for at least one agent by qPCR. Diseased dogs were most commonly positive for M. cynos (8/47, 17%), followed by CPIV (3/47, 6%) and B. bronchiseptica (3/47, 6%), while healthy dogs were most commonly positive for CAdV-2 (6/47, 13%), followed by M. cynos (2/47, 4%). All samples were negative for CIV, CRCoV, CDV and S. equi subsp. zooepidemicus. Viruses were not isolated from any of the samples tested. In total, 47/93 (50%) dogs were seropositive for CRCoV on at least one sampling occasion. Samples from diseased dogs were more frequently seropositive for CRCoV, with higher POI, than samples from healthy dogs. CONCLUSIONS AND CLINICAL RELEVANCE We showed that CAdV-2, CPIV, CHV, CRCoV, B. bronchiseptica and M. cynos circulated among sampled dogs. The convenience sampling methodology, with a poor match between the populations of diseased and healthy dogs in terms of age, breed and use, together with the relatively small sample size precluded inference of any causal relationships between infection with a given pathogen and development of disease. None-the-less, our data suggest that further investigation into epidemiology and disease association of CRCoV and M. cynos is warranted. In addition, circulation of novel respiratory pathogens among dogs in New Zealand should be considered in future studies, as 70/94 (74%) diseased dogs were negative for all the pathogens tested.

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Section: Discussionmentioning

confidence: 99%

A survey of canine respiratory pathogens in New Zealand dogs

Sowman

Cave

Dunowska

2018

New Zealand Veterinary Journal

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“…Over the past decades, PIV5 strains have been isolated from the respiratory system of different hosts [ 6 , 7 , 8 , 9 , 14 ]. Thus far, there is little information about PIV5 infection in the intestinal tissues of swine.…”

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confidence: 99%

Isolation and molecular characterization of parainfluenza virus 5 in diarrhea-affected piglets in China

Jiang

Wang

Guo

et al. 2018

The Journal of Veterinary Medical Science

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Recently, parainfluenza virus 5 (PIV5) infection has been increasingly reported in mammals. In this study, five PIV5 strains were isolated from diarrhea-affected piglets from four provinces or municipalities in China. An F-gene-based phylogenetic tree indicated that the five isolated strains were closely related to the PIV5 strain ZJQ-221 from a lesser panda in China, and the PIV5 strain 1168-1 from a dog in South Korea. The new isolates differed genetically from other pig, calf, rhesus macaque kidney cells, human, and dog PIV5 reference strains. Our study reveals the presence of PIV5 in intestinal tissue samples collected from diarrhea-affected piglets, and provides novel information regarding the epidemiology and tissue tropism of PIV5.

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“…Previous reports have shown isolation of MRuV5 strains from the respiratory system of several mammalian species [1,[5][6]28] and there are only a few reports on MRuV5 isolation from intestinal tissues or faecal samples [7]. Isolation of a cytopathogenic MRuV5 strain (SER) from a pig concurrently infected with porcine reproductive and respiratory syndrome virus in Germany has been reported [29].…”

Section: Discussionmentioning

confidence: 99%

First Report on Co-isolation and Whole-genomic Characterization of Mammalian Orthorubulavirus 5 and Mammalian Orthoreovirus Type-3 From Domestic Pigs in India

Singh

Rajukumar

Senthilkumar

et al. 2022

Preprint

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During a surveillance study to monitor porcine epidemic diarrohoea virus and transmissible gastroenteritis virus in India, a total of 1043 swine samples including faeces (n=264) and clotted blood (n=779) were collected and tested. Five samples (four faecal and one serum) showed cytopathic effects in Vero cells. Transmission electron microscopy of infective cell supernatant revealed the presence of two types of virions. Next generation sequencing (de novo) enabled complete genome assembly of Mammalian orthorubulavirus 5 (MRuV5; 15246 bp) and all 10 gene segments of Mammalian orthoreovirus (MRV; 22219 bp and 20512 bp). Genetic analysis of the MRuV5 revealed grouping of the Indian MRuV5 with those isolated from various mammalian species in South Korea and China, sharing more than 99% nucleotide identity. Deduced amino acid sequences of the HN, NP and F genes of MRuV5 isolates showed three (92L, 111R, 447H), two (86S, 121S) and two (139T, 246T) amino acid substitutions, respectively, compared to previously reported virus strains. The Indian MRV isolates were identified as MRV type-3 based on genetic analysis of S1 gene, showing the highest nucleotide identity (97.73%) with the MRV3 strain ZJ2013 isolated from pigs in China. Deduced amino acid sequences of MRV3 S1 gene revealed amino acid residues 198-204NLAIRLP, 249I, 340D, 419E known for sialic acid binding and neurotropism. We report the co-isolation and whole-genomic characterization of MRuV5 and MRV3 recorded incidentally for the first time from domestic pigs in India. It attracts attention to perform detailed surveillance studies and continuous monitoring of evolution and spread of emerging viruses, which may have pathogenic potential in animal and human hosts.

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Isolation and genomic characterization of a canine parainfluenza virus type 5 strain in China

Cited by 13 publications

References 25 publications

A survey of canine respiratory pathogens in New Zealand dogs

A survey of canine respiratory pathogens in New Zealand dogs

Isolation and molecular characterization of parainfluenza virus 5 in diarrhea-affected piglets in China

First Report on Co-isolation and Whole-genomic Characterization of Mammalian Orthorubulavirus 5 and Mammalian Orthoreovirus Type-3 From Domestic Pigs in India

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