Isolation and genomic characterization of a cypovirus from the oleander hawk moth, Daphnis nerii

Zhan, Zhigao; Guan, Limei; Wang, Jinchang; Liu, Zhuorong; Guo, Yan; Xiao, Yao; Wang, Hongxiu; Jin, Liang

doi:10.1016/j.jip.2019.03.002

Cited by 9 publications

(8 citation statements)

References 14 publications

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“…The same tissues were also collected from three uninfected control larvaes at the same time point. DnCPV was originally isolated from the larvae of D. nerii and propagated in D. nerii larvae as well [1]. The virion solution of DnCPV-23 utilized for infecting the D. nerii was stored at 4°C in the dark.…”

Section: Daphnis Nerii Larval Midgut and Virus Stockmentioning

confidence: 99%

“…The oleander hawk moth, Daphnis nerii (D. nerii), belongs to Lepidoptera, Sphingidae family, is a worldwide pest [1]. D. nerii larvae damages leave of Oleander, Catharanthus, Vinca, Adenium, Vitis, Tabernaemontana, Gardenia, Trachelospermum, Amsonia, Asclepias, Carissa, Rhazya, Thevetia, Jasminum and Ipomoea each year [2,3], which affect the landscape and the medicinal value of these plants.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional Responses of Daphnis nerii Larval Midgut to Oral Infection by 2 Daphnis nerii cypovirus-23

Kuang

Cheng-hua

Zhan

et al. 2021

Preprint

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Background: Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has lethal effect on the oleander hawk moth, Daphnis nerii , which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported.Methods: In order to better understand the pathogenic mechanism of DnCPV-23 infection, 3rdinstar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq.Results: The results showed that 1,979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, it was possible the function of food digestion and absorption in insect midgut was impaired after virus infection. In addition, the down-regulation of the immune response may be conducive to viral immune escape. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively. Conclusion: These results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.

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Section: Daphnis Nerii Larval Midgut and Virus Stockmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptional Responses of Daphnis nerii Larval Midgut to Oral Infection by 2 Daphnis nerii cypovirus-23

Kuang

Cheng-hua

Zhan

et al. 2021

Preprint

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“…The oleander hawk moth, Daphnis nerii ( D. nerii ), belongs to Lepidoptera, Sphingidae family, is a worldwide pest [ 1 ]. D. nerii larvae damages leave of Oleander, Catharanthus, Vinca, Adenium, Vitis, Tabernaemontana, Gardenia, Trachelospermum, Amsonia, Asclepias, Carissa, Rhazya, Thevetia, Jasminum and Ipomoea [ 2 , 3 ], which affect the landscape and the medicinal value of these plants.…”

Section: Introductionmentioning

confidence: 99%

“…DnCPV-23 was isolated from naturally diseased D. nerii larvae. This was a new type of cypovirus based on different electrophoretic migration patterns and conserved terminal sequences [ 1 , 5 , 6 ]. In addition to Daphnis nerii , it has been found that DnCPV-23 can also induce infection and death in many species of Sphingidae insects, such as Cephonodes hylas Linnaeus, Ampelophaga rubiginosa Bremer & Grey, and Agathia lycaenaria Kollar.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional responses of Daphnis nerii larval midgut to oral infection by Daphnis nerii cypovirus-23

Kuang

Yan

Zhan

et al. 2021

Virol J

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Background Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has a lethal effect on the oleander hawk moth, Daphnis nerii which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported. Methods To better understand the pathogenic mechanism of DnCPV-23 infection, 3rd-instar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq. Results The results showed that 1979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, we speculated that food digestion and absorption in insect midgut might be impaired after virus infection. In addition, the down-regulation of the immune response may make D. nerii more susceptible to bacterial infections. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively. Conclusion These results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.

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“…Cypovirus1 was collected from living and dead stem and cob borer larvae and pupae and examined by electron microscopy showed that particles of Cypovirus1 are shaped icosahedral, which belong to Reoviridae. Cyoovirus1 has a single-shelled capsid [10][11][12][13]. The Cypovirus1 infects the insect larvae of stored grain pests four days after inoculation with the Cypovirus1, causing chronic larvae disease and less-lethal chemical insecticides.…”

Section: Introductionmentioning

confidence: 99%

Biological Control Against Four-Stored Product Beetles Pests by Using Cytoplasmic Polyhedrosis Virus (Cypovirus1)

AL-Jaddawi¹,

Elbeshehy²,

Alkenani³

et al. 2021

Int j pharm phytopharm res

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The rice weevils, tobacco beetle, drug beetle, and Darkling beetle were collected from different locations in Mecca regions, KSA. The four different larvae attack stored products cause economic losses by incredible damage and post-harvest. This investigation was Carrie out to identify characters these insects include. In addition, Cypovirus1 was isolated and collected from living and dead stem and cob borer larvae and pupae from Maize in farmers' fields at Mecca regions, KSA. Samples have chronic diseases were tested by indirect ELISA (The enzyme-linked immunosorbent assay) and confirmed by TEM (Transmission electron microscopy) showing inclusion bodies demonstrated that the occlusion bodies were of irregular shape and ranged from 2.2 to 4.9 µm in diameter. On the other hand, we are studying the effects of different concentrations of a new Biocomponent of Cypovirus1 infection on the rice weevil adults, tobacco beetle, bread beetle, and darkling beetle larvae study their impact on the rates and severity of the infection. Also, the SDS-PAGE (Sodium dodecyl sulfatepolyacrylamide gel electrophoresis) test for showing various distinguishable sole bands in infected adults comparing with non-infected.

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Isolation and genomic characterization of a cypovirus from the oleander hawk moth, Daphnis nerii

Cited by 9 publications

References 14 publications

Transcriptional Responses of Daphnis nerii Larval Midgut to Oral Infection by 2 Daphnis nerii cypovirus-23

Transcriptional Responses of Daphnis nerii Larval Midgut to Oral Infection by 2 Daphnis nerii cypovirus-23

Transcriptional responses of Daphnis nerii larval midgut to oral infection by Daphnis nerii cypovirus-23

Biological Control Against Four-Stored Product Beetles Pests by Using Cytoplasmic Polyhedrosis Virus (Cypovirus1)

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