Isolation and Growth Characteristics of Psittacine Viruses in Chicken Embryos

Abstract

Eight viruses were isolated in embryonating eggs, from psittacine birds comprising a cockatiel, a budgerigar, 3 parrots, a love bird, and 2 rosellas. Initial attempts at isolation used 3 routes of embryo inoculation: yolk sac, allantoic sac, and chorioallantoic membrane (CAM). The most sensitive route was determined for 6 of the isolates by making comparative titrations by yolk sac, allantoic sac, and CAM routes of inoculation. In growth-curve studies of 6 of the isolates, virus concentration was determined daily for 5-6 days postinoculation in the CAM, allantoamnionic fluid, and liver. Peak virus concentrations appeared in about 72 hours. The embryonic lesions observed are described. Only one of the isolates hemagglutinated fowl red blood cells.

Molecular Genetic and Pathogenic Characterization of Psittacid Herpesvirus Type 1 Isolated from a Captive Galah (Eolophus roseicapillus) in Japan

“…2A). The RSL-1 strain (ATCC VR-915) [3], which is a reference strain for PsHV-1 in this study, was detected with all five primer sets. Tomaszewski et al reported that ten patterns (PsHV-1 variants v1 to v10) were observed using these five primer sets [12].…”

Development of an Experimental ELISA for the Detection of Antigens and Antibodies to the Virus of Pacheco Disease using Monoclonal Antibodies

“…Stressful situations such as exposure to sudden cold weather, or moving birds to an unfamiliar location are predisposing factors for the appearance of symptoms (CHEVILLE, 1978). For this reason, disease outbreaks are commonly observed in recently imported birds (HITCHNER and HIRAI, 1978;MILLER et al, 1979;. Sudden, deadly and unrestrained outbreaks of PPD may occur after the introduction of a new presumably infected psittacine bird (MARTIN et al, 1979;MILLER et al, 1979; GOMEZ-VILLAMANDOS, 1991), but sometimes in the resident birds of a flock with no history of recent introduction of new birds (SIMPSON and HANLEY, 1977).…”